Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 Nov 1;89(21):10552–10556. doi: 10.1073/pnas.89.21.10552

Human herpesvirus 7 is a T-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus.

Z N Berneman 1, D V Ablashi 1, G Li 1, M Eger-Fletcher 1, M S Reitz Jr 1, C L Hung 1, I Brus 1, A L Komaroff 1, R C Gallo 1
PMCID: PMC50377  PMID: 1332051

Abstract

An independent strain (JI) of human herpesvirus 7 (HHV-7) was isolated from a patient with chronic fatigue syndrome (CFS). No significant association could be established by seroepidemiology between HHV-7 and CFS. HHV-7 is a T-lymphotropic virus, infecting CD4+ and CD8+ primary lymphocytes. HHV-7 can also infect SUP-T1, an immature T-cell line, with variable success. Southern blot analysis with DNA probes scanning 58.8% of the human herpesvirus 6 (HHV-6) genome and hybridizing to all HHV-6 strains tested so far revealed homology to HHV-7 with only 37.4% of the total probe length. HHV-7 contains the GGGTTA repetitive sequence, as do HHV-6 and Marek's disease chicken herpesvirus. DNA sequencing of a 186-base-pair fragment of HHV-7(JI) revealed an identity with HHV-6 and human cytomegalovirus of 57.5% and 36%, respectively. Oligonucleotide primers derived from this sequence (HV7/HV8, HV10/HV11) amplified HHV-7 DNA only and did not amplify DNA from other human herpesviruses, including 12 different HHV-6 strains. Southern blot analysis with the p43L3 probe containing the 186-base-pair HHV-7 DNA fragment hybridized to HHV-7 DNA only. The molecular divergence between human cytomegalovirus, on the one hand, and HHV-6 and HHV-7, on the other, is greater than between HHV-6 and HHV-7, which, in turn, is greater than the difference between HHV-6 strains. This study supports the classification of HHV-7 as an additional member of the human beta-herpesviruses.

Full text

PDF
10554

Images in this article

10554Image
on p.10554
10554Image
on p.10554
10554Image
on p.10554
10555Image
on p.10555

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ablashi D. V., Josephs S. F., Buchbinder A., Hellman K., Nakamura S., Llana T., Lusso P., Kaplan M., Dahlberg J., Memon S. Human B-lymphotropic virus (human herpesvirus-6). J Virol Methods. 1988 Sep;21(1-4):29–48. doi: 10.1016/0166-0934(88)90050-x. [DOI] [PubMed] [Google Scholar]
  2. Balachandran N., Amelse R. E., Zhou W. W., Chang C. K. Identification of proteins specific for human herpesvirus 6-infected human T cells. J Virol. 1989 Jun;63(6):2835–2840. doi: 10.1128/jvi.63.6.2835-2840.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berneman Z. N., Gallo R. C., Ablashi D. V., Frenkel N., Katsafanas G., Kramarsky B., Brus I. Human herpesvirus 7 (HHV-7) strain JI: independent confirmation of HHV-7. J Infect Dis. 1992 Sep;166(3):690–691. doi: 10.1093/infdis/166.3.690. [DOI] [PubMed] [Google Scholar]
  4. Berneman Z. N., Gartenhaus R. B., Reitz M. S., Jr, Blattner W. A., Manns A., Hanchard B., Ikehara O., Gallo R. C., Klotman M. E. Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):3005–3009. doi: 10.1073/pnas.89.7.3005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carrigan D. R., Knox K. K., Tapper M. A. Suppression of human immunodeficiency virus type 1 replication by human herpesvirus-6. J Infect Dis. 1990 Oct;162(4):844–851. doi: 10.1093/infdis/162.4.844. [DOI] [PubMed] [Google Scholar]
  6. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A., 3rd, Kouzarides T., Martignetti J. A. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. doi: 10.1007/978-3-642-74980-3_6. [DOI] [PubMed] [Google Scholar]
  7. Chee M., Barrell B. Herpesviruses: a study of parts. Trends Genet. 1990 Mar;6(3):86–91. doi: 10.1016/0168-9525(90)90099-r. [DOI] [PubMed] [Google Scholar]
  8. Downing R. G., Sewankambo N., Serwadda D., Honess R., Crawford D., Jarrett R., Griffin B. E. Isolation of human lymphotropic herpesviruses from Uganda. Lancet. 1987 Aug 15;2(8555):390–390. doi: 10.1016/s0140-6736(87)92403-2. [DOI] [PubMed] [Google Scholar]
  9. Efstathiou S., Gompels U. A., Craxton M. A., Honess R. W., Ward K. DNA homology between a novel human herpesvirus (HHV-6) and human cytomegalovirus. Lancet. 1988 Jan 2;1(8575-6):63–64. doi: 10.1016/s0140-6736(88)91049-5. [DOI] [PubMed] [Google Scholar]
  10. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. Isolation of a new herpesvirus from human CD4+ T cells. Proc Natl Acad Sci U S A. 1990 Jan;87(2):748–752. doi: 10.1073/pnas.87.2.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Iyengar S., Levine P. H., Ablashi D., Neequaye J., Pearson G. R. Sero-epidemiological investigations on human herpesvirus 6 (HHV-6) infections using a newly developed early antigen assay. Int J Cancer. 1991 Oct 21;49(4):551–557. doi: 10.1002/ijc.2910490413. [DOI] [PubMed] [Google Scholar]
  12. Jahn G., Kouzarides T., Mach M., Scholl B. C., Plachter B., Traupe B., Preddie E., Satchwell S. C., Fleckenstein B., Barrell B. G. Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. J Virol. 1987 May;61(5):1358–1367. doi: 10.1128/jvi.61.5.1358-1367.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Josephs S. F., Ablashi D. V., Salahuddin S. Z., Jagodzinski L. L., Wong-Staal F., Gallo R. C. Identification of the human herpesvirus 6 glycoprotein H and putative large tegument protein genes. J Virol. 1991 Oct;65(10):5597–5604. doi: 10.1128/jvi.65.10.5597-5604.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Josephs S. F., Ablashi D. V., Salahuddin S. Z., Kramarsky B., Franza B. R., Jr, Pellett P., Buchbinder A., Memon S., Wong-Staal F., Gallo R. C. Molecular studies of HHV-6. J Virol Methods. 1988 Sep;21(1-4):179–190. doi: 10.1016/0166-0934(88)90064-x. [DOI] [PubMed] [Google Scholar]
  15. Josephs S. F., Salahuddin S. Z., Ablashi D. V., Schachter F., Wong-Staal F., Gallo R. C. Genomic analysis of the human B-lymphotropic virus (HBLV). Science. 1986 Oct 31;234(4776):601–603. doi: 10.1126/science.3020691. [DOI] [PubMed] [Google Scholar]
  16. Kikuta H., Lu H., Matsumoto S., Josephs S. F., Gallo R. C. Polymorphism of human herpesvirus 6 DNA from five Japanese patients with exanthem subitum. J Infect Dis. 1989 Sep;160(3):550–551. doi: 10.1093/infdis/160.3.550. [DOI] [PubMed] [Google Scholar]
  17. Kishi M., Harada H., Takahashi M., Tanaka A., Hayashi M., Nonoyama M., Josephs S. F., Buchbinder A., Schachter F., Ablashi D. V. A repeat sequence, GGGTTA, is shared by DNA of human herpesvirus 6 and Marek's disease virus. J Virol. 1988 Dec;62(12):4824–4827. doi: 10.1128/jvi.62.12.4824-4827.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Krueger G. R., Sander C., Hoffmann A., Barth A., Koch B., Braun M. Isolation of human herpesvirus-6 (HHV-6) from patients with collagen vascular diseases. In Vivo. 1991 May-Jun;5(3):217–225. [PubMed] [Google Scholar]
  19. Lawrence G. L., Chee M., Craxton M. A., Gompels U. A., Honess R. W., Barrell B. G. Human herpesvirus 6 is closely related to human cytomegalovirus. J Virol. 1990 Jan;64(1):287–299. doi: 10.1128/jvi.64.1.287-299.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Levy J. A., Ferro F., Lennette E. T., Oshiro L., Poulin L. Characterization of a new strain of HHV-6 (HHV-6SF) recovered from the saliva of an HIV-infected individual. Virology. 1990 Sep;178(1):113–121. doi: 10.1016/0042-6822(90)90384-4. [DOI] [PubMed] [Google Scholar]
  21. Lopez C., Pellett P., Stewart J., Goldsmith C., Sanderlin K., Black J., Warfield D., Feorino P. Characteristics of human herpesvirus-6. J Infect Dis. 1988 Jun;157(6):1271–1273. doi: 10.1093/infdis/157.6.1271. [DOI] [PubMed] [Google Scholar]
  22. Lusso P., Malnati M., De Maria A., Balotta C., DeRocco S. E., Markham P. D., Gallo R. C. Productive infection of CD4+ and CD8+ mature human T cell populations and clones by human herpesvirus 6. Transcriptional down-regulation of CD3. J Immunol. 1991 Jul 15;147(2):685–691. [PubMed] [Google Scholar]
  23. Lusso P., Markham P. D., Tschachler E., di Marzo Veronese F., Salahuddin S. Z., Ablashi D. V., Pahwa S., Krohn K., Gallo R. C. In vitro cellular tropism of human B-lymphotropic virus (human herpesvirus-6). J Exp Med. 1988 May 1;167(5):1659–1670. doi: 10.1084/jem.167.5.1659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Martin M. E., Thomson B. J., Honess R. W., Craxton M. A., Gompels U. A., Liu M. Y., Littler E., Arrand J. R., Teo I., Jones M. D. The genome of human herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. J Gen Virol. 1991 Jan;72(Pt 1):157–168. doi: 10.1099/0022-1317-72-1-157. [DOI] [PubMed] [Google Scholar]
  25. Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986 Oct 31;234(4776):596–601. doi: 10.1126/science.2876520. [DOI] [PubMed] [Google Scholar]
  26. Smith S. D., Shatsky M., Cohen P. S., Warnke R., Link M. P., Glader B. E. Monoclonal antibody and enzymatic profiles of human malignant T-lymphoid cells and derived cell lines. Cancer Res. 1984 Dec;44(12 Pt 1):5657–5660. [PubMed] [Google Scholar]
  27. Takahashi K., Sonoda S., Higashi K., Kondo T., Takahashi H., Takahashi M., Yamanishi K. Predominant CD4 T-lymphocyte tropism of human herpesvirus 6-related virus. J Virol. 1989 Jul;63(7):3161–3163. doi: 10.1128/jvi.63.7.3161-3163.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tedder R. S., Briggs M., Cameron C. H., Honess R., Robertson D., Whittle H. A novel lymphotropic herpesvirus. Lancet. 1987 Aug 15;2(8555):390–392. doi: 10.1016/s0140-6736(87)92404-4. [DOI] [PubMed] [Google Scholar]
  29. Wyatt L. S., Rodriguez W. J., Balachandran N., Frenkel N. Human herpesvirus 7: antigenic properties and prevalence in children and adults. J Virol. 1991 Nov;65(11):6260–6265. doi: 10.1128/jvi.65.11.6260-6265.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES