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. 1992 Nov 15;89(22):10568–10572. doi: 10.1073/pnas.89.22.10568

SpCOUP-TF: a sea urchin member of the steroid/thyroid hormone receptor family.

S M Chan 1, N Xu 1, C C Niemeyer 1, J R Bone 1, C N Flytzanis 1
PMCID: PMC50382  PMID: 1438252

Abstract

Embryonic nuclear proteins from the sea urchin Strongylocentrotus purpuratus bind in vitro to a cis-acting element that lies upstream of the actin gene CyIIIb and consists of two direct repeats homologous to steroid hormone response elements. This sea urchin element is specifically recognized by transcription factor COUP-TF from HeLa cell nuclear extracts as well. A sea urchin gene homologous to the human COUP-TF1 gene was detected by blot hybridization to S. purpuratus genomic DNA. Screening of a genomic DNA library with a human COUP-TF1 cDNA probe produced overlapping genomic clones that carry the S. purpuratus gene. Our results indicate that the sea urchin homologue has a structure similar to the human COUP-TF1 gene, i.e., conserved intron positions characteristic of this subgroup of steroid hormone receptors. Sequencing of the exons that encode the DNA- and ligand-binding domains of the sea urchin protein revealed 96% and 92% amino acid identity to the domains of the human protein, respectively. Transcripts derived from the S. purpuratus COUP-TF homologous gene were detected in ovarian and embryonic RNAs from various stages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagchi M. K., Tsai S. Y., Tsai M. J., O'Malley B. W. Purification and characterization of chicken ovalbumin gene upstream promoter transcription factor from homologous oviduct cells. Mol Cell Biol. 1987 Dec;7(12):4151–4158. doi: 10.1128/mcb.7.12.4151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Calzone F. J., Lee J. J., Le N., Britten R. J., Davidson E. H. A long, nontranslatable poly(A) RNA stored in the egg of the sea urchin Strongylocentrotus purpuratus. Genes Dev. 1988 Mar;2(3):305–318. doi: 10.1101/gad.2.3.305. [DOI] [PubMed] [Google Scholar]
  4. Chen E. Y., Seeburg P. H. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. doi: 10.1089/dna.1985.4.165. [DOI] [PubMed] [Google Scholar]
  5. Cooney A. J., Tsai S. Y., O'Malley B. W., Tsai M. J. Chicken ovalbumin upstream promoter transcription factor (COUP-TF) dimers bind to different GGTCA response elements, allowing COUP-TF to repress hormonal induction of the vitamin D3, thyroid hormone, and retinoic acid receptors. Mol Cell Biol. 1992 Sep;12(9):4153–4163. doi: 10.1128/mcb.12.9.4153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooney A. J., Tsai S. Y., O'Malley B. W., Tsai M. J. Chicken ovalbumin upstream promoter transcription factor binds to a negative regulatory region in the human immunodeficiency virus type 1 long terminal repeat. J Virol. 1991 Jun;65(6):2853–2860. doi: 10.1128/jvi.65.6.2853-2860.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cox K. H., Angerer L. M., Lee J. J., Davidson E. H., Angerer R. C. Cell lineage-specific programs of expression of multiple actin genes during sea urchin embryogenesis. J Mol Biol. 1986 Mar 20;188(2):159–172. doi: 10.1016/0022-2836(86)90301-3. [DOI] [PubMed] [Google Scholar]
  8. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  9. Evans R. M. The steroid and thyroid hormone receptor superfamily. Science. 1988 May 13;240(4854):889–895. doi: 10.1126/science.3283939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Flytzanis C. N., Bogosian E. A., Niemeyer C. C. Expression and structure of the CyIIIb actin gene of the sea urchin Strongylocentrotus purpuratus. Mol Reprod Dev. 1989;1(3):208–218. doi: 10.1002/mrd.1080010309. [DOI] [PubMed] [Google Scholar]
  11. Hwung Y. P., Wang L. H., Tsai S. Y., Tsai M. J. Differential binding of the chicken ovalbumin upstream promoter (COUP) transcription factor to two different promoters. J Biol Chem. 1988 Sep 15;263(26):13470–13474. [PubMed] [Google Scholar]
  12. JOLLEY W. B., MARTIN W. E., BAMBERGER J. W., STEARNS L. W. Effect of oestradiol-17beta on cleavage, nucleic acid metabolism and protein synthesis in sea urchin, Stronglylocentroutus purpuratus, embryos. J Endocrinol. 1962 Oct;25:183–188. doi: 10.1677/joe.0.0250183. [DOI] [PubMed] [Google Scholar]
  13. Ladias J. A., Karathanasis S. K. Regulation of the apolipoprotein AI gene by ARP-1, a novel member of the steroid receptor superfamily. Science. 1991 Feb 1;251(4993):561–565. doi: 10.1126/science.1899293. [DOI] [PubMed] [Google Scholar]
  14. Lee J. J., Shott R. J., Rose S. J., 3rd, Thomas T. L., Britten R. J., Davidson E. H. Sea urchin actin gene subtypes. Gene number, linkage and evolution. J Mol Biol. 1984 Jan 15;172(2):149–176. doi: 10.1016/s0022-2836(84)80035-2. [DOI] [PubMed] [Google Scholar]
  15. Lehoux J. G., Sandor T. The occurrence of steroids and steroid metabolizing enzyme systems in invertebrates. A review. Steroids. 1970 Aug;16(2):141–171. doi: 10.1016/s0039-128x(70)80102-7. [DOI] [PubMed] [Google Scholar]
  16. Leid M., Kastner P., Lyons R., Nakshatri H., Saunders M., Zacharewski T., Chen J. Y., Staub A., Garnier J. M., Mader S. Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently. Cell. 1992 Jan 24;68(2):377–395. doi: 10.1016/0092-8674(92)90478-u. [DOI] [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miyajima N., Kadowaki Y., Fukushige S., Shimizu S., Semba K., Yamanashi Y., Matsubara K., Toyoshima K., Yamamoto T. Identification of two novel members of erbA superfamily by molecular cloning: the gene products of the two are highly related to each other. Nucleic Acids Res. 1988 Dec 9;16(23):11057–11074. doi: 10.1093/nar/16.23.11057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mlodzik M., Hiromi Y., Weber U., Goodman C. S., Rubin G. M. The Drosophila seven-up gene, a member of the steroid receptor gene superfamily, controls photoreceptor cell fates. Cell. 1990 Jan 26;60(2):211–224. doi: 10.1016/0092-8674(90)90737-y. [DOI] [PubMed] [Google Scholar]
  20. Morris G. F., Marzluff W. F. A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III. Biochemistry. 1983 Feb 1;22(3):645–653. doi: 10.1021/bi00272a019. [DOI] [PubMed] [Google Scholar]
  21. Morris G. F., Price D. H., Marzluff W. F. Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3674–3678. doi: 10.1073/pnas.83.11.3674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Posakony J. W., Flytzanis C. N., Britten R. J., Davidson E. H. Interspersed sequence organization and developmental representation of cloned poly(A) RNAs from sea urchin eggs. J Mol Biol. 1983 Jun 25;167(2):361–389. doi: 10.1016/s0022-2836(83)80340-4. [DOI] [PubMed] [Google Scholar]
  23. Power R. F., Lydon J. P., Conneely O. M., O'Malley B. W. Dopamine activation of an orphan of the steroid receptor superfamily. Science. 1991 Jun 14;252(5012):1546–1548. doi: 10.1126/science.2047861. [DOI] [PubMed] [Google Scholar]
  24. Ritchie H. H., Wang L. H., Tsai S., O'Malley B. W., Tsai M. J. COUP-TF gene: a structure unique for the steroid/thyroid receptor superfamily. Nucleic Acids Res. 1990 Dec 11;18(23):6857–6862. doi: 10.1093/nar/18.23.6857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sagami I., Tsai S. Y., Wang H., Tsai M. J., O'Malley B. W. Identification of two factors required for transcription of the ovalbumin gene. Mol Cell Biol. 1986 Dec;6(12):4259–4267. doi: 10.1128/mcb.6.12.4259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Segraves W. A. Something old, some things new: the steroid receptor superfamily in Drosophila. Cell. 1991 Oct 18;67(2):225–228. doi: 10.1016/0092-8674(91)90172-u. [DOI] [PubMed] [Google Scholar]
  28. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  29. Umesono K., Murakami K. K., Thompson C. C., Evans R. M. Direct repeats as selective response elements for the thyroid hormone, retinoic acid, and vitamin D3 receptors. Cell. 1991 Jun 28;65(7):1255–1266. doi: 10.1016/0092-8674(91)90020-y. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wang L. H., Ing N. H., Tsai S. Y., O'Malley B. W., Tsai M. J. The COUP-TFs compose a family of functionally related transcription factors. Gene Expr. 1991;1(3):207–216. [PMC free article] [PubMed] [Google Scholar]
  31. Wang L. H., Tsai S. Y., Cook R. G., Beattie W. G., Tsai M. J., O'Malley B. W. COUP transcription factor is a member of the steroid receptor superfamily. Nature. 1989 Jul 13;340(6229):163–166. doi: 10.1038/340163a0. [DOI] [PubMed] [Google Scholar]
  32. Yu V. C., Delsert C., Andersen B., Holloway J. M., Devary O. V., När A. M., Kim S. Y., Boutin J. M., Glass C. K., Rosenfeld M. G. RXR beta: a coregulator that enhances binding of retinoic acid, thyroid hormone, and vitamin D receptors to their cognate response elements. Cell. 1991 Dec 20;67(6):1251–1266. doi: 10.1016/0092-8674(91)90301-e. [DOI] [PubMed] [Google Scholar]

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