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. 1992 Nov 15;89(22):11046–11050. doi: 10.1073/pnas.89.22.11046

The 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana.

X Liang 1, S Abel 1, J A Keller 1, N F Shen 1, A Theologis 1
PMCID: PMC50480  PMID: 1438312

Abstract

Genomic sequences encoding five divergent 1-aminocyclopropane-1-carboxylic acid (ACC) synthase polypeptides (ACC1, ACC2, ACC3, ACC4, and ACC5) have been isolated from Arabidopsis thaliana by using heterologous cDNAs and PCR fragments amplified from genomic DNA with degenerate oligonucleotide primers. Each gene is located on a different chromosome in the Arabidopsis genome. The genes are differentially expressed during development and in response to environmental stimuli. Protein-synthesis inhibition derepresses the expression of all genes but most dramatically derepresses that of ACC2, suggesting that their expression may be under negative control. The sequence of ACC2 was determined, and its transcription initiation site was defined. Authenticity of the polypeptide encoded by the gene was confirmed by expression experiments in Escherichia coli. The predicted size of the protein is 55,623 Da, and it contains the 11 invariant amino acid residues conserved between aminotransferases and ACC synthases from various plant species. Comparative analysis of structural and expression characteristics of ACC synthase genes from Arabidopsis and other plant species suggests that the sequence divergence of the ACC synthase genes and possibly the distinct regulatory networks governing the expression of ACC synthase subfamilies arose early in plant evolution and before the divergence of monocots and dicots.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atwater J. A., Wisdom R., Verma I. M. Regulated mRNA stability. Annu Rev Genet. 1990;24:519–541. doi: 10.1146/annurev.ge.24.120190.002511. [DOI] [PubMed] [Google Scholar]
  2. Breathnach R., Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. doi: 10.1146/annurev.bi.50.070181.002025. [DOI] [PubMed] [Google Scholar]
  3. Coruzzi G., Broglie R., Edwards C., Chua N. H. Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. EMBO J. 1984 Aug;3(8):1671–1679. doi: 10.1002/j.1460-2075.1984.tb02031.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dehesh K., Tepperman J., Christensen A. H., Quail P. H. phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet. 1991 Feb;225(2):305–313. doi: 10.1007/BF00269863. [DOI] [PubMed] [Google Scholar]
  5. Doolittle R. F., Feng D. F. Nearest neighbor procedure for relating progressively aligned amino acid sequences. Methods Enzymol. 1990;183:659–669. doi: 10.1016/0076-6879(90)83043-9. [DOI] [PubMed] [Google Scholar]
  6. Franco A. R., Gee M. A., Guilfoyle T. J. Induction and superinduction of auxin-responsive mRNAs with auxin and protein synthesis inhibitors. J Biol Chem. 1990 Sep 15;265(26):15845–15849. [PubMed] [Google Scholar]
  7. Fyrberg E. A., Mahaffey J. W., Bond B. J., Davidson N. Transcripts of the six Drosophila actin genes accumulate in a stage- and tissue-specific manner. Cell. 1983 May;33(1):115–123. doi: 10.1016/0092-8674(83)90340-9. [DOI] [PubMed] [Google Scholar]
  8. Guzmán P., Ecker J. R. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 1990 Jun;2(6):513–523. doi: 10.1105/tpc.2.6.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hightower R. C., Meagher R. B. Divergence and differential expression of soybean actin genes. EMBO J. 1985 Jan;4(1):1–8. doi: 10.1002/j.1460-2075.1985.tb02309.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huang P. L., Parks J. E., Rottmann W. H., Theologis A. Two genes encoding 1-aminocyclopropane-1-carboxylate synthase in zucchini (Cucurbita pepo) are clustered and similar but differentially regulated. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7021–7025. doi: 10.1073/pnas.88.16.7021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kim W. T., Silverstone A., Yip W. K., Dong J. G., Yang S. F. Induction of 1-aminocyclopropane-1-carboxylate synthase mRNA by auxin in mung bean hypocotyls and cultured apple shoots. Plant Physiol. 1992 Feb;98(2):465–471. doi: 10.1104/pp.98.2.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Liang X. W., Dron M., Cramer C. L., Dixon R. A., Lamb C. J. Differential regulation of phenylalanine ammonia-lyase genes during plant development and by environmental cues. J Biol Chem. 1989 Aug 25;264(24):14486–14492. [PubMed] [Google Scholar]
  13. Meyerowitz E. M., Pruitt R. E. Arabidopsis thaliana and Plant Molecular Genetics. Science. 1985 Sep 20;229(4719):1214–1218. doi: 10.1126/science.229.4719.1214. [DOI] [PubMed] [Google Scholar]
  14. Olson D. C., White J. A., Edelman L., Harkins R. N., Kende H. Differential expression of two genes for 1-aminocyclopropane-1-carboxylate synthase in tomato fruits. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5340–5344. doi: 10.1073/pnas.88.12.5340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Park K. Y., Drory A., Woodson W. R. Molecular cloning of an 1-aminocyclopropane-1-carboxylate synthase from senescing carnation flower petals. Plant Mol Biol. 1992 Jan;18(2):377–386. doi: 10.1007/BF00034964. [DOI] [PubMed] [Google Scholar]
  16. Rottmann W. H., Peter G. F., Oeller P. W., Keller J. A., Shen N. F., Nagy B. P., Taylor L. P., Campbell A. D., Theologis A. 1-aminocyclopropane-1-carboxylate synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and floral senescence. J Mol Biol. 1991 Dec 20;222(4):937–961. doi: 10.1016/0022-2836(91)90587-v. [DOI] [PubMed] [Google Scholar]
  17. Ryder T. B., Hedrick S. A., Bell J. N., Liang X. W., Clouse S. D., Lamb C. J. Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol Gen Genet. 1987 Dec;210(2):219–233. doi: 10.1007/BF00325687. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sato T., Theologis A. Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6621–6625. doi: 10.1073/pnas.86.17.6621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sen R., Baltimore D. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism. Cell. 1986 Dec 26;47(6):921–928. doi: 10.1016/0092-8674(86)90807-x. [DOI] [PubMed] [Google Scholar]
  21. Sharrock R. A., Quail P. H. Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev. 1989 Nov;3(11):1745–1757. doi: 10.1101/gad.3.11.1745. [DOI] [PubMed] [Google Scholar]
  22. Sugita M., Gruissem W. Developmental, organ-specific, and light-dependent expression of the tomato ribulose-1,5-bisphosphate carboxylase small subunit gene family. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7104–7108. doi: 10.1073/pnas.84.20.7104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Theologis A., Huynh T. V., Davis R. W. Rapid induction of specific mRNAs by auxin in pea epicotyl tissue. J Mol Biol. 1985 May 5;183(1):53–68. doi: 10.1016/0022-2836(85)90280-3. [DOI] [PubMed] [Google Scholar]
  24. Van der Straeten D., Van Wiemeersch L., Goodman H. M., Van Montagu M. Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4859–4863. doi: 10.1073/pnas.87.12.4859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilson A. C., Carlson S. S., White T. J. Biochemical evolution. Annu Rev Biochem. 1977;46:573–639. doi: 10.1146/annurev.bi.46.070177.003041. [DOI] [PubMed] [Google Scholar]
  26. Yip W. K., Dong J. G., Kenny J. W., Thompson G. A., Yang S. F. Characterization and sequencing of the active site of 1-aminocyclopropane-1-carboxylate synthase. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7930–7934. doi: 10.1073/pnas.87.20.7930. [DOI] [PMC free article] [PubMed] [Google Scholar]

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