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. 1995 Jan;79(1):70–75. doi: 10.1136/bjo.79.1.70

Pilot study on the effect of topical adrenergic medications on human Tenon's capsule fibroblasts in tissue culture.

I A Cunliffe 1, C A McIntyre 1, R C Rees 1, I G Rennie 1
PMCID: PMC505024  PMID: 7880797

Abstract

Recent publications have suggested that the long term use of topical antiglaucoma medications may be detrimental to the outcome of trabeculectomy. In order to investigate this further, the effect of several adrenergic agents and a preservative on the proliferation and viability of human Tenon's capsule fibroblasts in tissue culture were examined. The following compounds were tested: adrenaline (Eppy 1% and pure adrenaline base 1%, Smith & Nephew Pharmaceuticals Ltd); dipivefrine hydrochloride (Propine 0.1% and pure dipivefrine hydrochloride 0.1% Allergan Ltd and Allergan Pharmaceuticals (Ireland) Ltd); benzalkonium chloride (pure benzalkonium chloride 0.01%, Sigma Chemical Company Ltd); the two adrenaline based preparations were also tested in the presence of an antioxidant. None of the tested compounds stimulated the proliferation of fibroblasts. The commercial products tested, their pure compounds, and the preservative all inhibited proliferation and had toxic effects on the cells. In the presence of antioxidant, commercial Eppy and pure adrenaline base appeared to have less effect on proliferation and toxicity. These findings are discussed with reference to the outcome of trabeculectomy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Batterbury M., Wishart P. K. Is high initial aqueous outflow of benefit in trabeculectomy? Eye (Lond) 1993;7(Pt 1):109–112. doi: 10.1038/eye.1993.23. [DOI] [PubMed] [Google Scholar]
  2. Burstein N. L. Preservative cytotoxic threshold for benzalkonium chloride and chlorhexidine digluconate in cat and rabbit corneas. Invest Ophthalmol Vis Sci. 1980 Mar;19(3):308–313. [PubMed] [Google Scholar]
  3. Cairns J. E. Surgical treatment of primary open-angle glaucoma. Trans Ophthalmol Soc U K. 1972;92:745–756. [PubMed] [Google Scholar]
  4. Coleiro J. A., Sigurdsson H., Lockyer J. A. Follicular conjunctivitis on Dipivefrin therapy for glaucoma. Eye (Lond) 1988;2(Pt 4):440–442. doi: 10.1038/eye.1988.80. [DOI] [PubMed] [Google Scholar]
  5. Collin H. B. Ultrastructural changes to corneal stromal cells due to ophthalmic preservatives. Acta Ophthalmol (Copenh) 1986 Feb;64(1):72–78. doi: 10.1111/j.1755-3768.1986.tb06875.x. [DOI] [PubMed] [Google Scholar]
  6. Fiore P. M., Jacobs I. H., Goldberg D. B. Drug-induced pemphigoid. A spectrum of diseases. Arch Ophthalmol. 1987 Dec;105(12):1660–1663. doi: 10.1001/archopht.1987.01060120058023. [DOI] [PubMed] [Google Scholar]
  7. Hitchings R. A. Primary surgery for primary open angle glaucoma--justified or not? Br J Ophthalmol. 1993 Jul;77(7):445–448. doi: 10.1136/bjo.77.7.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoffer K. J. Pemphigoid related to epinephrine treatment. Am J Ophthalmol. 1977 Apr;83(4):601–601. doi: 10.1016/0002-9394(77)90577-3. [DOI] [PubMed] [Google Scholar]
  9. Kohn A. N., Moss A. P., Hargett N. A., Ritch R., Smith H., Jr, Podos S. M. Clinical comparison of dipivalyl epinephrine and epinephrine in the treatment of glaucoma. Am J Ophthalmol. 1979 Feb;87(2):196–201. doi: 10.1016/0002-9394(79)90142-9. [DOI] [PubMed] [Google Scholar]
  10. Krejci L., Harrison R. Antiglaucoma drug effects on corneal epithelium. A comparative study in tissue culture. Arch Ophthalmol. 1970 Dec;84(6):766–769. doi: 10.1001/archopht.1970.00990040768015. [DOI] [PubMed] [Google Scholar]
  11. Kristensen E. B., Norn M. S. Benign mucous membrane pemphigoid. 1. Secretion of mucus and tears. Acta Ophthalmol (Copenh) 1974;52(2):266–281. doi: 10.1111/j.1755-3768.1974.tb00377.x. [DOI] [PubMed] [Google Scholar]
  12. Lavin M. J., Wormald R. P., Migdal C. S., Hitchings R. A. The influence of prior therapy on the success of trabeculectomy. Arch Ophthalmol. 1990 Nov;108(11):1543–1548. doi: 10.1001/archopht.1990.01070130045027. [DOI] [PubMed] [Google Scholar]
  13. Liesegang T. J. Bulbar conjunctival follicles associated with dipivefrin therapy. Ophthalmology. 1985 Feb;92(2):228–233. doi: 10.1016/s0161-6420(85)34048-4. [DOI] [PubMed] [Google Scholar]
  14. Longstaff S., Wormald R. P., Mazover A., Hitchings R. A. Glaucoma triple procedures: efficacy of intraocular pressure control and visual outcome. Ophthalmic Surg. 1990 Nov;21(11):786–793. [PubMed] [Google Scholar]
  15. Migdal C., Hitchings R. Control of chronic simple glaucoma with primary medical, surgical and laser treatment. Trans Ophthalmol Soc U K. 1986;105(Pt 6):653–656. [PubMed] [Google Scholar]
  16. Miller M. H., Rice N. S. Trabeculectomy combined with beta irradiation for congenital glaucoma. Br J Ophthalmol. 1991 Oct;75(10):584–590. doi: 10.1136/bjo.75.10.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pfister R. R., Burstein N. The effects of ophthalmic drugs, vehicles, and preservatives on corneal epithelium: a scanning electron microscope study. Invest Ophthalmol. 1976 Apr;15(4):246–259. [PubMed] [Google Scholar]
  18. Podos S. M. Pharmacology of ocular drugs: 2. Epinephrine. Ophthalmology. 1980 Jul;87(7):721–723. doi: 10.1016/s0161-6420(80)35176-2. [DOI] [PubMed] [Google Scholar]
  19. Pouliquen Y., Patey A., Foster C. S., Goichot L., Savoldelli M. Drug-induced cicatricial pemphigoid affecting the conjunctiva. Light and electron microscopic features. Ophthalmology. 1986 Jun;93(6):775–783. doi: 10.1016/s0161-6420(86)33675-3. [DOI] [PubMed] [Google Scholar]
  20. Samples J. R., Binder P. S., Nayak S. The effect of epinephrine and benzalkonium chloride on cultured corneal endothelial and trabecular meshwork cells. Exp Eye Res. 1989 Jul;49(1):1–12. doi: 10.1016/0014-4835(89)90071-7. [DOI] [PubMed] [Google Scholar]
  21. Schwab I. R., Linberg J. V., Gioia V. M., Benson W. H., Chao G. M. Foreshortening of the inferior conjunctival fornix associated with chronic glaucoma medications. Ophthalmology. 1992 Feb;99(2):197–202. doi: 10.1016/s0161-6420(92)32001-9. [DOI] [PubMed] [Google Scholar]
  22. Sherwood M. B., Grierson I., Millar L., Hitchings R. A. Long-term morphologic effects of antiglaucoma drugs on the conjunctiva and Tenon's capsule in glaucomatous patients. Ophthalmology. 1989 Mar;96(3):327–335. doi: 10.1016/s0161-6420(89)32888-0. [DOI] [PubMed] [Google Scholar]
  23. Smith D. L., Skuta G. L., Kincaid M. C., Rabbani R., Cruess D. F., Kao S. F. The effects of glaucoma medications on Tenon's capsule and conjunctiva in the rabbit. Ophthalmic Surg. 1991 Jun;22(6):336–340. [PubMed] [Google Scholar]
  24. Theodore J., Leibowitz H. M. External ocular toxicity of dipivalyl epinephrine. Am J Ophthalmol. 1979 Dec;88(6):1013–1016. doi: 10.1016/0002-9394(79)90407-0. [DOI] [PubMed] [Google Scholar]
  25. Tripathi B. J., Tripathi R. C., Kolli S. P. Cytotoxicity of ophthalmic preservatives on human corneal epithelium. Lens Eye Toxic Res. 1992;9(3-4):361–375. [PubMed] [Google Scholar]
  26. Tripathi B. J., Tripathi R. C., Millard C. B. Epinephrine-induced toxicity of human trabecular cells in vitro. Lens Eye Toxic Res. 1989;6(1-2):141–156. [PubMed] [Google Scholar]
  27. Williams D. E., Nguyen K. D., Shapourifar-Tehrani S., Kitada S., Lee D. A. Effects of timolol, betaxolol, and levobunolol on human tenon's fibroblasts in tissue culture. Invest Ophthalmol Vis Sci. 1992 Jun;33(7):2233–2241. [PubMed] [Google Scholar]
  28. Wilson F. M., 2nd Adverse external ocular effects of topical ophthalmic medications. Surv Ophthalmol. 1979 Sep-Oct;24(2):57–88. doi: 10.1016/0039-6257(79)90125-5. [DOI] [PubMed] [Google Scholar]
  29. Wilson F. M., 2nd Adverse external ocular effects of topical ophthalmic therapy: an epidemiologic, laboratory, and clinical study. Trans Am Ophthalmol Soc. 1983;81:854–965. [PMC free article] [PubMed] [Google Scholar]
  30. Wright P. Cicatrizing conjunctivitis. Trans Ophthalmol Soc U K. 1986;105(Pt 1):1–17. [PubMed] [Google Scholar]
  31. Wright P. Squamous metaplasia or epidermalization of the conjunctiva as an adverse reaction to topical medication. Trans Ophthalmol Soc U K. 1979 Jul;99(2):244–246. [PubMed] [Google Scholar]
  32. Young T. L., Higginbotham E. J., Zou X. L., Farber M. D. Effects of topical glaucoma drugs on fistulized rabbit conjunctiva. Ophthalmology. 1990 Nov;97(11):1423–1427. doi: 10.1016/s0161-6420(90)32392-8. [DOI] [PubMed] [Google Scholar]

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