Skip to main content
PMC home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 1995 Oct;79(10):939–943. doi: 10.1136/bjo.79.10.939

Types of collagen synthesised by the lens epithelial cells of human cataracts.

O Nishi 1, K Nishi 1, T Fujiwara 1, E Shirasawa 1
PMCID: PMC505297  PMID: 7488584

Abstract

AIMS/BACKGROUND--Residual lens epithelial cells (LECs) undergo fibrous proliferation after cataract surgery, resulting in capsular fibrosis. The purpose of this study was to determine the types of collagen produced in cultured LECs derived from human cataract LECs. METHODS--A circular section of the anterior capsule, about 5 mm in diameter, with LECs attached was obtained by anterior capsulotomy during cataract surgery and cultured directly without dispersion of the cells in a well, on the bottom of which a disc-shaped, thin plate of poly(methyl methacrylate) had been placed. At 5 to 6 weeks of culture, the proliferated cells of the culture were stained immunohistochemically with antibodies against human collagens I-VI by the avidin-biotin-peroxidase complex method. RESULTS--Collagens I, IV, V, and VI were positive in the cultured cells. Types IV and V were strongly present in almost all the cells whereas types I and VI were only observed in a few cells. Collagens II and III were negative. CONCLUSIONS--Since the lens capsule is known to be comprised of collagen IV, collagens I, V, and VI seem to be produced newly in culture. The capsular fibrosis seen after cataract surgery in vivo as a wound healing process of the lens capsule, may contain these types of collagens. The present culture model is useful for studying secondary cataract formation.

Full text

PDF
941

Images in this article

940Image
on p.940
940Image
on p.940
941Image
on p.941
941Image
on p.941
942Image
on p.942
942Image
on p.942

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apple D. J., Solomon K. D., Tetz M. R., Assia E. I., Holland E. Y., Legler U. F., Tsai J. C., Castaneda V. E., Hoggatt J. P., Kostick A. M. Posterior capsule opacification. Surv Ophthalmol. 1992 Sep-Oct;37(2):73–116. doi: 10.1016/0039-6257(92)90073-3. [DOI] [PubMed] [Google Scholar]
  2. Arita T., Murata Y., Lin L. R., Tsuji T., Reddy V. N. Synthesis of lens capsule in long-term culture of human lens epithelial cells. Invest Ophthalmol Vis Sci. 1993 Feb;34(2):355–362. [PubMed] [Google Scholar]
  3. Barnard K., Gathercole L. J., Bailey A. J. Basement membrane collagen--evidence for a novel molecular packing. FEBS Lett. 1987 Feb 9;212(1):49–52. doi: 10.1016/0014-5793(87)81554-5. [DOI] [PubMed] [Google Scholar]
  4. Cobo L. M., Ohsawa E., Chandler D., Arguello R., George G. Pathogenesis of capsular opacification after extracapsular cataract extraction. An animal model. Ophthalmology. 1984 Jul;91(7):857–863. doi: 10.1016/s0161-6420(84)34225-7. [DOI] [PubMed] [Google Scholar]
  5. Fagerholm P. P., Philipson B. T. Formation of after-cataract by regeneration of human and rabbit lens epithelium in tissue culture. Acta Ophthalmol (Copenh) 1977 Jun;55(3):362–371. doi: 10.1111/j.1755-3768.1977.tb06110.x. [DOI] [PubMed] [Google Scholar]
  6. Font R. L., Brownstein S. A light and electron microscopic study of anterior subcapsular cataracts. Am J Ophthalmol. 1974 Dec;78(6):972–984. doi: 10.1016/0002-9394(74)90811-3. [DOI] [PubMed] [Google Scholar]
  7. Frezzotti R., Caporossi A., Mastrangelo D., Hadjistilianou T., Tosi P., Cintorino M., Minacci C. Pathogenesis of posterior capsular opacification. Part II: Histopathological and in vitro culture findings. J Cataract Refract Surg. 1990 May;16(3):353–360. doi: 10.1016/s0886-3350(13)80708-0. [DOI] [PubMed] [Google Scholar]
  8. Hatae T., Ishibashi T., Yoshitomi F., Shibata Y. Immunocytochemistry of types I-IV collagen in human anterior subcapsular cataracts. Graefes Arch Clin Exp Ophthalmol. 1993 Oct;231(10):586–590. doi: 10.1007/BF00936523. [DOI] [PubMed] [Google Scholar]
  9. Hettlich H. J., Wenzel M., Janssen M., Mittermayer C. Immunohistochemische Untersuchungen der menschlichen Linsenkapsel. Fortschr Ophthalmol. 1990;87(2):147–149. [PubMed] [Google Scholar]
  10. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  11. Jacob T. J., Humphry R. C., Davies E. G., Thompson G. M. Cytological factors relating to posterior capsule opacification following cataract surgery. Br J Ophthalmol. 1987 Sep;71(9):659–663. doi: 10.1136/bjo.71.9.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laurent M., Kern P., Courtois Y., Regnault F. Synthesis of types I, III an IV collagen by bovine lens epithelial cells in long-term culture. Exp Cell Res. 1981 Jul;134(1):23–31. doi: 10.1016/0014-4827(81)90459-6. [DOI] [PubMed] [Google Scholar]
  13. Marshall G. E., Konstas A. G., Bechrakis N. E., Lee W. R. An immunoelectron microscope study of the aged human lens capsule. Exp Eye Res. 1992 Mar;54(3):393–401. doi: 10.1016/0014-4835(92)90051-s. [DOI] [PubMed] [Google Scholar]
  14. Marshall G. E., Konstas A. G., Lee W. R. Collagens in ocular tissues. Br J Ophthalmol. 1993 Aug;77(8):515–524. doi: 10.1136/bjo.77.8.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McDonnell P. J., Zarbin M. A., Green W. R. Posterior capsule opacification in pseudophakic eyes. Ophthalmology. 1983 Dec;90(12):1548–1553. doi: 10.1016/s0161-6420(83)34350-5. [DOI] [PubMed] [Google Scholar]
  16. Mohan P. S., Spiro R. G. Macromolecular organization of basement membranes. Characterization and comparison of glomerular basement membrane and lens capsule components by immunochemical and lectin affinity procedures. J Biol Chem. 1986 Mar 25;261(9):4328–4336. [PubMed] [Google Scholar]
  17. Nishi O., Nishi K. Disruption of the blood-aqueous barrier by residual lens epithelial cells after intraocular lens implantation. Ophthalmic Surg. 1992 May;23(5):325–329. [PubMed] [Google Scholar]
  18. Nishi O., Nishi K., Imanishi M. Synthesis of interleukin-1 and prostaglandin E2 by lens epithelial cells of human cataracts. Br J Ophthalmol. 1992 Jun;76(6):338–341. doi: 10.1136/bjo.76.6.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nishi O., Nishi K., Sakka Y., Sakuraba T., Maeda S. Intercapsular cataract surgery with lens epithelial cell removal. Part IV: Capsular fibrosis induced by poly(methyl methacrylate). J Cataract Refract Surg. 1991 Jul;17(4):471–477. doi: 10.1016/s0886-3350(13)80854-1. [DOI] [PubMed] [Google Scholar]
  20. Scheinman J. I., Tsai C. Monoclonal antibody to type IV collagen with selective basement membrane localization. Lab Invest. 1984 Jan;50(1):101–112. [PubMed] [Google Scholar]
  21. Schmut O. The organization of tissues of the eye by different collagen types. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Aug 16;207(3):189–199. doi: 10.1007/BF00411053. [DOI] [PubMed] [Google Scholar]
  22. Sundarraj N., Willson J. Monoclonal antibody to human basement membrane collagen type IV. Immunology. 1982 Sep;47(1):133–140. [PMC free article] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES