Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Feb 15;88(4):1446–1450. doi: 10.1073/pnas.88.4.1446

Characterization of a variant of human T-lymphotropic virus type I isolated from a healthy member of a remote, recently contacted group in Papua New Guinea.

R Yanagihara 1, V R Nerurkar 1, R M Garruto 1, M A Miller 1, M E Leon-Monzon 1, C L Jenkins 1, R C Sanders 1, P P Liberski 1, M P Alpers 1, D C Gajdusek 1
PMCID: PMC51035  PMID: 1996344

Abstract

We report the characterization of a variant of human T-lymphotropic virus type I (HTLV-I) isolated from an interleukin 2-dependent, CD8+ T-cell line derived from peripheral blood mononuclear cells of a healthy member of a remote, recently contacted hunter-horticulturalist group (Hagahai) in Madang province of Papua New Guinea. Antigenic characterization of this variant, designated PNG-1, by immunofluorescence, indicated no expression of gag-encoded proteins p19 and p24 (even after incubation with 5-bromo-2'-deoxyuridine), using monoclonal and polyclonal antibodies against HTLV-I gag gene products. Virus-specific proteins of 15, 19, 46, 53, and 61/68 kDa were demonstrated by Western blot analysis, using sera from patients with serologically and/or virologically confirmed HTLV-I myeloneuropathy, sera from HTLV-I-infected rabbits, and antibodies prepared against the C terminus of the major envelope glycoprotein gp46. Restriction endonuclease maps of PNG-1 proviral DNA differed from that of a prototype strain of HTLV-I (MT-2), but, as verified by polymerase chain reaction, PNG-1 was definitely HTLV-I, not HTLV-II. Nucleotide sequencing and further molecular genetic studies of this variant may provide insights into the origin and evolution of HTLV-I.

Full text

PDF
1450

Images in this article

1448Image
on p.1448
1448Image
on p.1448
1448Image
on p.1448
1449Image
on p.1449
1449Image
on p.1449
1449Image
on p.1449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ajdukiewicz A., Yanagihara R., Garruto R. M., Gajdusek D. C., Alexander S. S. HTLV-1 myeloneuropathy in the Solomon Islands. N Engl J Med. 1989 Aug 31;321(9):615–616. doi: 10.1056/NEJM198908313210913. [DOI] [PubMed] [Google Scholar]
  2. Armstrong M. Y., Hrdy D. B., Carlson J. R., Friedlaender J. S. Prevalence of antibodies interactive with HTLV-I antigens in selected Solomon Islands populations. Am J Phys Anthropol. 1990 Apr;81(4):465–470. doi: 10.1002/ajpa.1330810403. [DOI] [PubMed] [Google Scholar]
  3. Asher D. M., Goudsmit J., Pomeroy K. L., Garruto R. M., Bakker M., Ono S. G., Elliot N., Harris K., Askins H., Eldadah Z. Antibodies to HTLV-I in populations of the southwestern Pacific. J Med Virol. 1988 Dec;26(4):339–351. doi: 10.1002/jmv.1890260402. [DOI] [PubMed] [Google Scholar]
  4. Babona D. V., Nurse G. T. HTLV-I antibodies in Papua New Guinea. Lancet. 1988 Nov 12;2(8620):1148–1148. doi: 10.1016/s0140-6736(88)90573-9. [DOI] [PubMed] [Google Scholar]
  5. Bhatia K., Jenkins C., Prasad M., Koki G., Lombange J. Immunogenetic studies of two recently contacted populations from Papua New Guinea. Hum Biol. 1989 Feb;61(1):45–64. [PubMed] [Google Scholar]
  6. Brabin L., Brabin B. J., Doherty R. R., Gust I. D., Alpers M. P., Fujino R., Imai J., Hinuma Y. Patterns of migration indicate sexual transmission of HTLV-I infection in non-pregnant women in Papua New Guinea. Int J Cancer. 1989 Jul 15;44(1):59–62. doi: 10.1002/ijc.2910440111. [DOI] [PubMed] [Google Scholar]
  7. Brindle R. J., Eglin R. P., Parsons A. J., Hill A. V., Selkon J. B. HTLV-1, HIV-1, hepatitis B and hepatitis delta in the Pacific and South-East Asia: a serological survey. Epidemiol Infect. 1988 Feb;100(1):153–156. doi: 10.1017/s095026880006564x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cartier-Rovirosa L., Mora C., Araya F., Castillo J., Verdugo R., Miller M. A., Gajdusek D. C., Gibbs C. J., Jr HTLV-I positive spastic paraparesis in a temperate zone. Lancet. 1989 Mar 11;1(8637):556–557. doi: 10.1016/s0140-6736(89)90098-6. [DOI] [PubMed] [Google Scholar]
  9. De B. K., Srinivasan A. Multiple primer pairs for the detection of HTLV-I by PCR. Nucleic Acids Res. 1989 Mar 11;17(5):2142–2142. doi: 10.1093/nar/17.5.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gessain A., Saal F., Giron M. L., Lasneret J., Lagaye S., Gout O., De Thé G., Sigaux F., Peries J. Cell surface phenotype and human T lymphotropic virus type 1 antigen expression in 12 T cell lines derived from peripheral blood and cerebrospinal fluid of West Indian, Guyanese and African patients with tropical spastic paraparesis. J Gen Virol. 1990 Feb;71(Pt 2):333–341. doi: 10.1099/0022-1317-71-2-333. [DOI] [PubMed] [Google Scholar]
  11. Hinuma Y., Nagata K., Hanaoka M., Nakai M., Matsumoto T., Kinoshita K. I., Shirakawa S., Miyoshi I. Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476–6480. doi: 10.1073/pnas.78.10.6476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Huet T., Dazza M. C., Brun-Vézinet F., Roelants G. E., Wain-Hobson S. A highly defective HIV-1 strain isolated from a healthy Gabonese individual presenting an atypical western blot. AIDS. 1989 Nov;3(11):707–715. doi: 10.1097/00002030-198911000-00004. [DOI] [PubMed] [Google Scholar]
  13. Jenkins C. L. Health in the early contact period: a contemporary example from Papua New Guinea. Soc Sci Med. 1988;26(10):997–1006. doi: 10.1016/0277-9536(88)90217-1. [DOI] [PubMed] [Google Scholar]
  14. Kazura J. W., Saxinger W. C., Wenger J., Forsyth K., Lederman M. M., Gillespie J. A., Carpenter C. C., Alpers M. A. Epidemiology of human T cell leukemia virus type I infection in East Sepik Province, Papua New Guinea. J Infect Dis. 1987 Jun;155(6):1100–1107. doi: 10.1093/infdis/155.6.1100. [DOI] [PubMed] [Google Scholar]
  15. Kwok S., Kellogg D., Ehrlich G., Poiesz B., Bhagavati S., Sninsky J. J. Characterization of a sequence of human T cell leukemia virus type I from a patient with chronic progressive myelopathy. J Infect Dis. 1988 Dec;158(6):1193–1197. doi: 10.1093/infdis/158.6.1193. [DOI] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lee H., Swanson P., Shorty V. S., Zack J. A., Rosenblatt J. D., Chen I. S. High rate of HTLV-II infection in seropositive i.v. drug abusers in New Orleans. Science. 1989 Apr 28;244(4903):471–475. doi: 10.1126/science.2655084. [DOI] [PubMed] [Google Scholar]
  18. Markham P. D., Salahuddin S. Z., Kalyanaraman V. S., Popovic M., Sarin P., Gallo R. C. Infection and transformation of fresh human umbilical cord blood cells by multiple sources of human T-cell leukemia-lymphoma virus (HTLV). Int J Cancer. 1983 Apr 15;31(4):413–420. doi: 10.1002/ijc.2910310404. [DOI] [PubMed] [Google Scholar]
  19. McKhann G., 2nd, Gibbs C. J., Jr, Mora C. A., Rodgers-Johnson P. E., Liberski P. P., Gdula W. J., Zaninovic V. Isolation and characterization of HTLV-I from symptomatic family members with tropical spastic paraparesis (HTLV-I encephalomyeloneuropathy). J Infect Dis. 1989 Sep;160(3):371–379. doi: 10.1093/infdis/160.3.371. [DOI] [PubMed] [Google Scholar]
  20. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki Y., Shiraishi Y., Nagata K., Hinuma Y. Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature. 1981 Dec 24;294(5843):770–771. doi: 10.1038/294770a0. [DOI] [PubMed] [Google Scholar]
  21. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Popovic M., Lange-Wantzin G., Sarin P. S., Mann D., Gallo R. C. Transformation of human umbilical cord blood T cells by human T-cell leukemia/lymphoma virus. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5402–5406. doi: 10.1073/pnas.80.17.5402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Re M. C., Tommaseo M., Furlini G., La Placa M. High prevalence of serum antibody against human T cell leukemia virus type I (HTLV-I) among the Bismam Asmat population (Indonesian New Guinea). AIDS Res Hum Retroviruses. 1989 Oct;5(5):551–554. doi: 10.1089/aid.1989.5.551. [DOI] [PubMed] [Google Scholar]
  24. Reddy E. P., Sandberg-Wollheim M., Mettus R. V., Ray P. E., DeFreitas E., Koprowski H. Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients. Science. 1989 Jan 27;243(4890):529–533. doi: 10.1126/science.2536193. [DOI] [PubMed] [Google Scholar]
  25. Richardson J. H., Edwards A. J., Cruickshank J. K., Rudge P., Dalgleish A. G. In vivo cellular tropism of human T-cell leukemia virus type 1. J Virol. 1990 Nov;64(11):5682–5687. doi: 10.1128/jvi.64.11.5682-5687.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Salahuddin S. Z., Markham P. D., Wong-Staal F., Franchini G., Kalyanaraman V. S., Gallo R. C. Restricted expression of human T-cell leukemia--lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes. Virology. 1983 Aug;129(1):51–64. doi: 10.1016/0042-6822(83)90395-1. [DOI] [PubMed] [Google Scholar]
  27. Sanders R. C., Levin A., Anian G., Webber I., Lee H., Swanson P., Diwan A., Desowitz R., Blattner W. A., Alpers M. P. HTLV-I antibody studies in villagers in East Sepik Province, Papua New Guinea. Arch Virol. 1990;114(1-2):27–35. doi: 10.1007/BF01311009. [DOI] [PubMed] [Google Scholar]
  28. Seiki M., Hattori S., Hirayama Y., Yoshida M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618–3622. doi: 10.1073/pnas.80.12.3618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wain-Hobson S. HIV genome variability in vivo. AIDS. 1989;3 (Suppl 1):S13–S18. doi: 10.1097/00002030-198901001-00003. [DOI] [PubMed] [Google Scholar]
  30. Weber J. N., Banatvala N., Clayden S., McAdam K. P., Palmer S., Moulsdale H., Tosswill J., Dilger P., Thorpe R., Amann S. HTLV-1 infection in Papua New Guinea: evidence for serologic false positivity. J Infect Dis. 1989 Jun;159(6):1025–1028. doi: 10.1093/infdis/159.6.1025. [DOI] [PubMed] [Google Scholar]
  31. Yanagihara R., Garruto R. M., Miller M. A., Leon-Monzon M., Liberski P. P., Gajdusek D. C., Jenkins C. L., Sanders R. C., Alpers M. P. Isolation of HTLV-I from members of a remote tribe in New Guinea. N Engl J Med. 1990 Oct 4;323(14):993–994. doi: 10.1056/NEJM199010043231413. [DOI] [PubMed] [Google Scholar]
  32. Yanagihara R., Jenkins C. L., Alexander S. S., Mora C. A., Garruto R. M. Human T lymphotropic virus type I infection in Papua New Guinea: high prevalence among the Hagahai confirmed by western analysis. J Infect Dis. 1990 Sep;162(3):649–654. doi: 10.1093/infdis/162.3.649. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES