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. 1976 Feb;17(2):352–362. doi: 10.1128/jvi.17.2.352-362.1976

Endonucleases and simian virus 40 virions: origin of a virion-associated endonuclease.

D C Dooley, M T Ryzlak, H L Ozer
PMCID: PMC515426  PMID: 176420

Abstract

The origin and role of the endonuclease activity associated with purified virions of simian virus 40, previously described by this and other laboratories, have been further investigated. We found that the enzymatic activity from virions of temperature-sensitive (ts) mutants is not more heat labile than that from wild-type virions. This result was obtained for a variety of ts mutants, including three of the tsA class, and in experiments in which the enzyme was tested in both the presence and absence of viral particles. Comparison of the viron enzyme with endonucleases prepared from either serum or nuclei of uninfected cells reveals a similarity between the viron and serum enzymes based on chromatographic behavior and relative activity with different cations. Virus particles prepared free of this endonuclease were still infectious. We were unsuccessful in uncovering endonuclease in such preparations upon disruption. These data emphasize the necessity for caution in interpreting the role of particle-associated enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderer F. A., Koch M. A., Schlumberger H. D. Structure of simian virus 40. 3. Alkaline degradation of the virus particle. Virology. 1968 Mar;34(3):452–458. doi: 10.1016/0042-6822(68)90065-2. [DOI] [PubMed] [Google Scholar]
  2. BURKHARD R. K., KROPF G. SOLUBILIZATION OF MITOCHONDRIAL PROTEINS. Biochim Biophys Acta. 1964 Aug 19;90:393–396. doi: 10.1016/0304-4165(64)90203-x. [DOI] [PubMed] [Google Scholar]
  3. Beard P., Morrow J. F., Berg P. Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease. J Virol. 1973 Dec;12(6):1303–1313. doi: 10.1128/jvi.12.6.1303-1313.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burlingham B. T., Doerfler W., Pettersson U., Philipson L. Adenovirus endonuclease: association with the penton of adenovirus type 2. J Mol Biol. 1971 Aug 28;60(1):45–64. doi: 10.1016/0022-2836(71)90446-3. [DOI] [PubMed] [Google Scholar]
  5. Chou J. Y., Avila J., Martin R. G. Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40. J Virol. 1974 Jul;14(1):116–124. doi: 10.1128/jvi.14.1.116-124.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cuzin F., Blangy D., Rouget P. Activité endonucléasique de préparations purifiées du virus du Polyome. C R Acad Sci Hebd Seances Acad Sci D. 1971 Dec 20;273(25):2650–2653. [PubMed] [Google Scholar]
  7. Di Mayorca G., Callender J., Marin G., Giordano R. Temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):126–133. doi: 10.1016/0042-6822(69)90134-2. [DOI] [PubMed] [Google Scholar]
  8. Eckhart W. Complementation and transformation by temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):120–125. doi: 10.1016/0042-6822(69)90133-0. [DOI] [PubMed] [Google Scholar]
  9. FRIED M. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Mar;53:486–491. doi: 10.1073/pnas.53.3.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GEIDUSCHEK E. P., DANIELS A. A SIMPLE ASSAY FOR DNA ENDONUCLEASES. Anal Biochem. 1965 Apr;11:133–137. doi: 10.1016/0003-2697(65)90052-7. [DOI] [PubMed] [Google Scholar]
  11. Greenaway P. J. A study on the cleavage site specificity of the endonuclease associated with purified simian virus 40 particles. FEBS Lett. 1973 Aug 15;34(2):193–197. doi: 10.1016/0014-5793(73)80791-4. [DOI] [PubMed] [Google Scholar]
  12. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  13. Kaplan J. C., Wilbert S. M., Black P. H. Endonuclease activity associated with purified simian virus 40 virions. J Virol. 1972 May;9(5):800–803. doi: 10.1128/jvi.9.5.800-803.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kidwell W. R., Saral R., Martin R. G., Ozer H. L. Characterization of an endonuclease associated with simian virus 40 virions. J Virol. 1972 Sep;10(3):410–416. doi: 10.1128/jvi.10.3.410-416.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Laval F. Endonuclease activity associated with purified PM2 bacteriophages. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4965–4969. doi: 10.1073/pnas.71.12.4965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mandel J. L., Chambon P. Animal DNA-dependent RNA polymerases. Studies on the reaction parameters of transcription in vitro of Simian virus 40 DNA by mammalian RNA polymerases AI and B. Eur J Biochem. 1974 Jan 16;41(2):367–378. doi: 10.1111/j.1432-1033.1974.tb03279.x. [DOI] [PubMed] [Google Scholar]
  18. Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
  19. Ozer H. L. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. J Virol. 1972 Jan;9(1):41–51. doi: 10.1128/jvi.9.1.41-51.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ozer H. L., Takemoto K. K., Kirschstein R. L., Axelrod D. Immunochemical characterization of plaque mutants of simian virus 40. J Virol. 1969 Jan;3(1):17–24. doi: 10.1128/jvi.3.1.17-24.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Paolette E., Rosemond-Hornbeak H., Moss B. Two nucleid acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus. Purification and characterization. J Biol Chem. 1974 May 25;249(10):3273–3280. [PubMed] [Google Scholar]
  22. Parodi A., Rouget P., Croissant O., Blangy D., Cuzin F. Endonucleolytic cleavage of polyoma virus DNA: general properties and site specificity of the virion-associated endonuclease. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):247–254. doi: 10.1101/sqb.1974.039.01.032. [DOI] [PubMed] [Google Scholar]
  23. Robb J. A., Martin R. G. Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus. Virology. 1970 Aug;41(4):751–760. doi: 10.1016/0042-6822(70)90439-3. [DOI] [PubMed] [Google Scholar]
  24. Rosemond-Hornbeak H., Paoletti E., Moss B. Single-stranded deoxyribonucleic acid-specific nuclease from vaccinia virus. Purification and characterization. J Biol Chem. 1974 May 25;249(10):3287–3291. [PubMed] [Google Scholar]
  25. Tegtmeyer P., Ozer H. L. Temperature-sensitive mutants of simian virus 40: infection of permissive cells. J Virol. 1971 Oct;8(4):516–524. doi: 10.1128/jvi.8.4.516-524.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Walter G., Roblin R., Dulbecco R. Protein synthesis in Simian virus 40-infected monkey cells. Proc Natl Acad Sci U S A. 1972 Apr;69(4):921–924. doi: 10.1073/pnas.69.4.921. [DOI] [PMC free article] [PubMed] [Google Scholar]

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