Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Mar;17(3):686–691. doi: 10.1128/jvi.17.3.686-691.1976

Biochemical, biophysical, and biological properties of densonucleosis virus. I. Structural proteins.

P Tijssen, J van den Hurk, E Kurstak
PMCID: PMC515466  PMID: 1255854

Abstract

The polypeptide composition of highly purified densonucleosis virus was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The viral proteins showed a different behavior in sodium dodecyl sulfate-gels in comparison with the marker proteins. Therefore, the molecular weights were estimated by analyzing the retardation of the electrophoretic mobility of these proteins in gels with increasing polyacrylamide concentrations. Four structural proteins with molecular weights of 49,000, 58,500, 69,000, and 98,000 were found, ant they were designated p49, p59, p69, and p98, respectively. There are several indications that p98 is a dimer of p49. The relative quantity of the structural proteins in a virion suggests that at least p49 (accounting for +/-70% of total protein mass) is a capsid protein and that there will be 12 capsomers per virion.

Full text

PDF
687

Images in this article

688Image
on p.688

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J., Scott K. J. Quantitative staining of fraction I protein in polyacrylamide gels using Coomassie brillant blue. Anal Biochem. 1971 Sep;43(1):173–182. doi: 10.1016/0003-2697(71)90122-9. [DOI] [PubMed] [Google Scholar]
  2. Geelen J. L., van Kammen A., Verduin B. J. Structure of the capsid of cowpea mosaic virus. The chemical subunit: molecular weight and number of subunits per particle. Virology. 1972 Jul;49(1):205–213. doi: 10.1016/s0042-6822(72)80022-9. [DOI] [PubMed] [Google Scholar]
  3. Gorovsky M. A., Carlson K., Rosenbaum J. L. Simple method for quantitive densitometry of polyacrylamide gels using fast green. Anal Biochem. 1970 Jun;35(2):359–370. doi: 10.1016/0003-2697(70)90196-x. [DOI] [PubMed] [Google Scholar]
  4. Johnson F. B., Hoggan M. D. Structural proteins of HADEN virus. Virology. 1973 Jan;51(1):129–137. doi: 10.1016/0042-6822(73)90373-5. [DOI] [PubMed] [Google Scholar]
  5. Johnson F. B., Ozer H. L., Hoggan M. D. Structural proteins of adenovirus-associated virus type 3. J Virol. 1971 Dec;8(6):860–863. doi: 10.1128/jvi.8.6.860-863.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kongsvik J. R., Toolan H. W. Capsid components of the parvovirus H-1. Proc Soc Exp Biol Med. 1972 Apr;139(4):1202–1205. doi: 10.3181/00379727-139-36329. [DOI] [PubMed] [Google Scholar]
  7. Kurstak E., Côté J. R. Proposition de classification du virus de la densonucléose (VDN) basée sur l'étude de la structure moléculaire et des propriétés physicochimiques. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jan 20;268(3):616–619. [PubMed] [Google Scholar]
  8. Kurstak E. Small DNA densonucleosis virus (DNV). Adv Virus Res. 1972;17:207–241. doi: 10.1016/s0065-3527(08)60751-4. [DOI] [PubMed] [Google Scholar]
  9. Kurstak E., Vernoux J. P., Brakier-Gingras L. Etude biophysique de l'acide désoxyribonucléique de virus de la densonucléose (VDN). II. Extraction du DNA viral et mise en évidence de la présence de chaînes polynucléotidiques complémentairs, encapsidées séparément dans les virions VD. Arch Gesamte Virusforsch. 1973;40(3):274–284. [PubMed] [Google Scholar]
  10. Rose J. A., Maizel J. V., Jr, Inman J. K., Shatkin A. J. Structural proteins of adenovirus-associated viruses. J Virol. 1971 Nov;8(5):766–770. doi: 10.1128/jvi.8.5.766-770.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salzman L. A., White W. L. Structural proteins of Kilham rat virus. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1551–1556. doi: 10.1016/0006-291x(70)90564-4. [DOI] [PubMed] [Google Scholar]
  12. Tinsley T. W., Longworth J. F. Parvoviruses. J Gen Virol. 1973 Jun;20(Suppl):7–15. doi: 10.1099/0022-1317-20-Supplement-7. [DOI] [PubMed] [Google Scholar]
  13. Truffaut N., Berger G., Niveleau A., May P., Bergoin M., Vago C. Recherches sur l'acide nucléique du virus de la densonucléose du lepidoptère Galleria mellonella L. Arch Gesamte Virusforsch. 1967;21(3):469–474. [PubMed] [Google Scholar]
  14. Ugel A. R., Chrambach A., Rodbard D. Fractionation and characterization of an oligomeric series of bovine keratohyalin by polyacrylamide gel electrophoresis. Anal Biochem. 1971 Oct;43(2):410–426. doi: 10.1016/0003-2697(71)90271-5. [DOI] [PubMed] [Google Scholar]
  15. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES