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. 1976 Mar;17(3):794–800. doi: 10.1128/jvi.17.3.794-800.1976

Late events in T4 bacteriophage production. I. Late DNA replication is primarily exponential.

L D Kosturko, A W Kozinski
PMCID: PMC515478  PMID: 1255858

Abstract

The possibility of a switch in the mechanism of T4 DNA replication, from an exponential-bidirectional mode at early stages to a nonexponential (rolling circle) mode at later stages of phage development, has been investigated. The conclusion that DNA replication does not involve such a change in mechanism for the majority of replicating molecules is based on the analysis of the clonal distribution of mutants specifically induced at late times after infection. The clonal distribution of mutants, induced by adding 5-bromodeoxyuridine to infected cells at a time when 100 phage equivalents of DNA had accumulated, fits the pattern predicted by exponential replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernstein H., Bernstein C. Circular and branched circular concatenates as possible intermediates in bacteriophage T4 DNA replication. J Mol Biol. 1973 Jul 5;77(3):355–361. doi: 10.1016/0022-2836(73)90443-9. [DOI] [PubMed] [Google Scholar]
  2. Delius H., Howe C., Kozinski A. W. Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3049–3053. doi: 10.1073/pnas.68.12.3049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doermann A. H. T4 and the rolling circle model of replication. Annu Rev Genet. 1973;7:325–341. doi: 10.1146/annurev.ge.07.120173.001545. [DOI] [PubMed] [Google Scholar]
  4. Emanuel B. S. Replicative hybrid of T4 bacteriophage DNA. J Virol. 1973 Aug;12(2):408–412. doi: 10.1128/jvi.12.2.408-412.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Freese E. THE DIFFERENCE BETWEEN SPONTANEOUS AND BASE-ANALOGUE INDUCED MUTATIONS OF PHAGE T4. Proc Natl Acad Sci U S A. 1959 Apr;45(4):622–633. doi: 10.1073/pnas.45.4.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  7. Howe C. C., Buckley P. J., Carlson K. M., Kozinski A. W. Multiple and specific initiation of T4 DNA replication. J Virol. 1973 Jul;12(1):130–148. doi: 10.1128/jvi.12.1.130-148.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KOZINSKI A. W. Fragmentary transfer of P32-labeled parental DNA to progeny phage. Virology. 1961 Jan;13:124–134. doi: 10.1016/0042-6822(61)90039-3. [DOI] [PubMed] [Google Scholar]
  9. KOZINSKI A. W., KOZINSKI P. B. Fragmentary transfer of P32-labeled parental DNA to progeny phage. II. The average size of the transferred parental fragment. Two-cycletransfer. Repair of the polynucleotide chain after fragmentation. Virology. 1963 Jun;20:213–229. doi: 10.1016/0042-6822(63)90109-0. [DOI] [PubMed] [Google Scholar]
  10. Kozinski A. W., Kozinski P. B., James R. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J Virol. 1967 Aug;1(4):758–770. doi: 10.1128/jvi.1.4.758-770.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kozinski A. W., Kozinski P. B., Shannon P. REPLICATIVE FRAGMENTATION IN T4 PHAGE: INHIBITION BY CHLORAMPHENICOL. Proc Natl Acad Sci U S A. 1963 Oct;50(4):746–753. doi: 10.1073/pnas.50.4.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LURIA S. E. The frequency distribution of spontaneous bacteriophage mutants as evidence for the exponential rate of phage reproduction. Cold Spring Harb Symp Quant Biol. 1951;16:463–470. doi: 10.1101/sqb.1951.016.01.033. [DOI] [PubMed] [Google Scholar]

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