Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Apr;18(1):34–41. doi: 10.1128/jvi.18.1.34-41.1976

Proteins solubilized from frog virus 3 particles: effect on transcription.

A M Aubertin, C Travo, A Kirn
PMCID: PMC515518  PMID: 1255875

Abstract

The treatment of KB cells with viral proteins solubilized from frog virus 3 particles (SVE) induced a rapid shutoff of host RNA synthesis. The RNA polymerase activities of SVE-treated cells were drastically depressed, corresonding, at least for RNA polymerase B, to a decrease in the number of enzyme molecules. In vitro, SVE had no direct effect on RNA polymerases but was capable of binding with calf thymus DNA to form an SVE-DNA complex modifying the template capacity. The effect of SVE on a transcription system consisting of cell lysates would suggest that cytoplasmic factors are necessary for expression of the inhibitory capacity of SVE.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubertin A. M., Decker C., Kirn A. Effect of gamma-rays on infectivity and capacity for nuclear-associated and cytoplasmic DNA replication of FV 3 in chick embryo fibroblasts. Radiat Res. 1970 Oct;44(1):178–186. [PubMed] [Google Scholar]
  2. Aubertin A. M., Hirth C., Travo C., Nonnenmacher H., Kirn A. Preparation and properties of an inhibitory extract from frog virus 3 particles. J Virol. 1973 May;11(5):694–701. doi: 10.1128/jvi.11.5.694-701.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bingen-Brendel A., Batzenschlager A., Gut J. P., Hirth C., Vetter J. M., Kirn A. Etude histologique et virologique de l'hépatite dégénérative aigue provoquée par le FV3 (Frog Virus 3) chez la souris. Ann Inst Pasteur (Paris) 1972 Jan;122(1):125–142. [PubMed] [Google Scholar]
  4. Campadelli-Fiume G., Bragaglia M. M., Costanzo F., Foà L., LaPlaca M. Early ultrastructural changes induced in BHK cell nuclei by frog virus 3. A possible mechanism for the impairment of cellular DNA synthesis. J Gen Virol. 1973 Oct;21:197–200. doi: 10.1099/0022-1317-21-1-197. [DOI] [PubMed] [Google Scholar]
  5. Campadelli-Fiume G., Costanzo F., Foa'-Tomasi L., La Placa M. Modifications of cellular RNA-polymerase II after infection with frog virus 3. J Gen Virol. 1975 Jun;27(3):391–394. doi: 10.1099/0022-1317-27-3-391. [DOI] [PubMed] [Google Scholar]
  6. Chambon P. Eukaryotic nuclear RNA polymerases. Annu Rev Biochem. 1975;44:613–638. doi: 10.1146/annurev.bi.44.070175.003145. [DOI] [PubMed] [Google Scholar]
  7. Cochet-Meilhac M., Chambon P. Animal DNA-dependent RNA polymerases. 11. Mechanism of the inhibition of RNA polymerases B by amatoxins. Biochim Biophys Acta. 1974 Jun 27;353(2):160–184. doi: 10.1016/0005-2787(74)90182-8. [DOI] [PubMed] [Google Scholar]
  8. Costanzo F., La Placa M., Palenzoma A. M., Novello F., Stirpe F. Selective inhibition of a nuclear RNA polymerase in cells infected with frog virus. Nature. 1970 Jul 18;227(5255):294–295. doi: 10.1038/227294a0. [DOI] [PubMed] [Google Scholar]
  9. Elharrar M., Hirth C., Blanc J., Kirn A. Pathogénie de l'hépatite toxique de la souris provoquée par le FV 3 (frog virus 3): inhibition de la synthèse des macromolécules du foie. Biochim Biophys Acta. 1973 Aug 10;319(1):91–102. [PubMed] [Google Scholar]
  10. FRANKLIN R. M., BALTIMORE D. Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harb Symp Quant Biol. 1962;27:175–198. doi: 10.1101/sqb.1962.027.001.019. [DOI] [PubMed] [Google Scholar]
  11. Guir J., Braunwald J., Kirn A. Inhibition of host-specific DNA and RNA synthesis in KB cells following infection with frog virus 3. J Gen Virol. 1971 Sep;12(3):293–301. doi: 10.1099/0022-1317-12-3-293. [DOI] [PubMed] [Google Scholar]
  12. Jacob S. T., Sajdel E. M., Munro H. N. Different responses of soluble whole nuclear RNA polymerase and soluble nucleolar RNA polymerase to divalent cations and to inhibition by alpha-amanitin. Biochem Biophys Res Commun. 1970 Feb 20;38(4):765–770. doi: 10.1016/0006-291x(70)90647-9. [DOI] [PubMed] [Google Scholar]
  13. Kelly D. C. Frog virus 3 replication: electron microscope observations on the sequence of infection in chick embryo fibroblasts. J Gen Virol. 1975 Jan;26(1):71–86. doi: 10.1099/0022-1317-26-1-71. [DOI] [PubMed] [Google Scholar]
  14. Maes R., Granoff A. Viruses and renal carcinoma of Rana pipiens. IV. Nucleic acid synthesis in frog virus 3-infected BHK 21/13 cells. Virology. 1967 Nov;33(3):491–502. doi: 10.1016/0042-6822(67)90125-0. [DOI] [PubMed] [Google Scholar]
  15. McAuslan B. R., Smith W. R. Deoxyribonucleic acid synthesis in FV-3-infected mammalian cells. J Virol. 1968 Oct;2(10):1006–1015. doi: 10.1128/jvi.2.10.1006-1015.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morrison J. F. Chromatographic separation of nucleoside phosphates on diethylaminoethylcellulose paper. Anal Biochem. 1968 Jul;24(1):106–111. doi: 10.1016/0003-2697(68)90064-x. [DOI] [PubMed] [Google Scholar]
  17. Schwartz L. B., Lawrence C., Thach R. E., Roeder R. G. Encephalomyocarditis virus infection of mouse plasmacytoma cells. II. Effect on host RNA synthesis and RNA polymerases. J Virol. 1974 Sep;14(3):611–619. doi: 10.1128/jvi.14.3.611-619.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wu G. J., Zubay G. Prolonged transcription in a cell-free system involving nuclei and cytoplasm. Proc Natl Acad Sci U S A. 1974 May;71(5):1803–1807. doi: 10.1073/pnas.71.5.1803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yu F. L., Feigelson P. The rapid turnover of RNA polymerase of rat liver nucleolus, and of its messenger RNA. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2833–2837. doi: 10.1073/pnas.69.10.2833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yu F. L. Two functional states of the RNA polymerases in the rat hepatic nuclear and nucleolar fractions. Nature. 1974 Sep 27;251(5473):344–346. doi: 10.1038/251344a0. [DOI] [PubMed] [Google Scholar]
  21. Zylber-Katz E., Weisman P. The Chanock Centre for Virology, Hebrew University. Hadassah Medical School, Jerusalem, Israel. J Gen Virol. 1974 Dec;25(3):405–413. doi: 10.1099/0022-1317-25-3-405. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES