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. 1976 Apr;18(1):176–181. doi: 10.1128/jvi.18.1.176-181.1976

Autologous immune responses to the major oncornavirus polypeptides in unmanipulated AKR/J mice.

M B Oldstone, B C Del Villano, F J Dixon
PMCID: PMC515536  PMID: 176459

Abstract

The autologous immune response of AKR/J mice to the structural proteins of murine leukemia virus (MuLV) was examined. Immunoglobulins from the renal glomeruli were chemically eluted, separated from antigens, recovered, and tested for immunological reactivity against MuLV structural proteins. Analyzing immune precipitates obtained after mixing radiolabeled Tween-disrupted MuLV preparations with eluates from AKR/J mice on sodium dodecyl sulfategel electrophoresis, we found evidence of antibodies to the major classes of MuLV structural components: gp70, gp45, p30, and one or more proteins in the 10,000- to 15,000-dalton class. Using rate zonal centrifugation we confirmed that the eluates from AKR/J glomeruli contained antibody(s) that bound specifically to p30. These results indicate that AKR/J mice spontaneously mount immune responses against the major oncornavirus polypeptide antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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