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. 1976 Apr;18(1):205–210. doi: 10.1128/jvi.18.1.205-210.1976

Chemical modification of simian virus 40 DNA by reaction with a water-soluble carbodiimide.

J Lebowitz, C G Garon, M C Chen, N P Salzman
PMCID: PMC515539  PMID: 176461

Abstract

Superhelical simian virus 40 (SV40) DNA I can be modified with N-cyclohexyl-N'-beta-(4 methylmorpholinium)ethylcarbodiimide (CMC). The reaction produces an increase in the sedimentation velocity of DNA I from 21 to 22.5S and a decrease in its buoyant density in CsCl from 1.694 to 1.688. A comparable shift in buoyant density is observed in a saturated ethidium bromide-cesium chloride gradient where form II, which has been exposed to CMC, shows no shift. The CsCl-buoyant density data allows us to estimate that 108 mol of CMC are bound per mol of SV40 DNA I. In the subsequent paper an alternative procedure has been used to locate CMC sites, and the extent of the regions available to bind CMC have been measured.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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