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. 1976 May;18(2):596–603. doi: 10.1128/jvi.18.2.596-603.1976

RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants.

D M Hunt, S U Emerson, R R Wagner
PMCID: PMC515586  PMID: 178900

Abstract

In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted transcriptions of the group I RNA-ts mutants. NS protein activity, but not L protein activity, was purified from the group I ts mutants; this NS fraction always displayed the wt phenotype in reconstitution assays. Neither the L nor the NS protein was capable of restoring the defective transcriptive activity of the group IV vesicular stomatitis virus mutant ts W16B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop D. H. Complete transcription by the transcriptase of vesicular stomatitis virus. J Virol. 1971 Apr;7(4):486–490. doi: 10.1128/jvi.7.4.486-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop D. H., Emerson S. U., Flamand A. Reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (Indiana serotype), Chandipura, vesicular stomatitis (New Jersey serotype), and Cocal viruses. J Virol. 1974 Jul;14(1):139–144. doi: 10.1128/jvi.14.1.139-144.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bishop D. H., Roy P. Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase. J Virol. 1972 Aug;10(2):234–243. doi: 10.1128/jvi.10.2.234-243.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Both G. W., Moyer S. A., Banerjee A. K. Translation and identification of the viral mRNA species isolated from subcellular fractions of vesicular stomatitis virus-infected cells. J Virol. 1975 Apr;15(4):1012–1019. doi: 10.1128/jvi.15.4.1012-1019.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cairns J. E., Holloway A. F., Cormack D. V. Temperature-sensitive mutants of vesicular stomatitis virus: in vitro studies of virion-associated polymerase. J Virol. 1972 Dec;10(6):1130–1135. doi: 10.1128/jvi.10.6.1130-1135.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Combard A., Martinet C., Printz Ane C., Friedman A., Printz P. Transcription and replication of vesicular stomatitis virus: effects of temperature-sensitive mutations in complementation group IV. J Virol. 1974 Apr;13(4):922–930. doi: 10.1128/jvi.13.4.922-930.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cormack D. V., Holloway A. F., Pringle C. R. Temperature-sensitive mutants of vesicular stomatitis virus; homology and nomenclature. J Gen Virol. 1973 Jun;19(3):295–300. doi: 10.1099/0022-1317-19-3-295. [DOI] [PubMed] [Google Scholar]
  9. Emerson S. U., Wagner R. R. Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions. J Virol. 1972 Aug;10(2):297–309. doi: 10.1128/jvi.10.2.297-309.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Emerson S. U., Wagner R. R. L protein requirement for in vitro RNA synthesis by vesicular stomatitis virus. J Virol. 1973 Dec;12(6):1325–1335. doi: 10.1128/jvi.12.6.1325-1335.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Emerson S. U., Yu Y. Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. J Virol. 1975 Jun;15(6):1348–1356. doi: 10.1128/jvi.15.6.1348-1356.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Flamand A., Bishop D. H. Primary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups. J Virol. 1973 Dec;12(6):1238–1252. doi: 10.1128/jvi.12.6.1238-1252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Flamand A., Pringle C. R. The homologies of spontaneous and induced temperature-sensitive mutants of vesicular stomatitis virus isolated in chick embryo and BHK 21 cells. J Gen Virol. 1971 May;11(2):81–85. doi: 10.1099/0022-1317-11-2-81. [DOI] [PubMed] [Google Scholar]
  14. Huang A. S., Baltimore D., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus. 3. Multiple complementary messenger RNA molecules. Virology. 1970 Dec;42(4):946–957. doi: 10.1016/0042-6822(70)90343-0. [DOI] [PubMed] [Google Scholar]
  15. Hunt D. M., Wagner R. R. Location of the transcription defect in group I temperature-sensitive mutants of vesicular stomatitis virus. J Virol. 1974 Jan;13(1):28–35. doi: 10.1128/jvi.13.1.28-35.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knipe D., Rose J. K., Lodish H. F. Translation of individual species of vesicular stomatitis viral mRNA. J Virol. 1975 Apr;15(4):1004–1011. doi: 10.1128/jvi.15.4.1004-1011.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Mudd J. A., Summers D. F. Polysomal ribonucleic acid of vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Dec;42(4):958–968. doi: 10.1016/0042-6822(70)90344-2. [DOI] [PubMed] [Google Scholar]
  19. Ngan J. S., Holloway A. F., Cormack D. V. Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of group I and group IV mutants. J Virol. 1974 Oct;14(4):765–772. doi: 10.1128/jvi.14.4.765-772.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perlman S. M., Huang A. S. RNA synthesis of vesicular stomatitis virus. V. Interactions between transcription and replication. J Virol. 1973 Dec;12(6):1395–1400. doi: 10.1128/jvi.12.6.1395-1400.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pringle C. R. Conditional lethal mutants of vesicular stomatitis virus. Curr Top Microbiol Immunol. 1975;69:85–116. doi: 10.1007/978-3-642-50112-8_2. [DOI] [PubMed] [Google Scholar]
  22. Pringle C. R., Duncan I. B. Preliminary physiological characterization of temperature-sensitive mutants of vesicular stomatitis virus. J Virol. 1971 Jul;8(1):56–61. doi: 10.1128/jvi.8.1.56-61.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Printz-Ané C., Combard A., Martinet C. Study of the transcription and the replication of vesicular stomatitis virus by using temperature-sensitive mutants. J Virol. 1972 Nov;10(5):889–895. doi: 10.1128/jvi.10.5.889-895.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rettenmier C. W., Dumont R., Baltimore D. Screening procedure for complementation-dependent mutants of vesicular stomatitis virus. J Virol. 1975 Jan;15(1):41–49. doi: 10.1128/jvi.15.1.41-49.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Szilágyi J. F., Pringle C. R. Effect of temperature-sensitive mutations on the virion-associated RNA transcriptase of vesicular stomatitis virus. J Mol Biol. 1972 Nov 14;71(2):281–291. doi: 10.1016/0022-2836(72)90351-8. [DOI] [PubMed] [Google Scholar]
  26. Szilágyi J. F., Uryvayev L. Isolation of an infectious ribonucleoprotein from vesicular stomatitis virus containing an active RNA transcriptase. J Virol. 1973 Feb;11(2):279–286. doi: 10.1128/jvi.11.2.279-286.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WAGNER R. R., LEVEY A. H., SNYDER R. M., RATCLIFF G. A., Jr, HYATT D. F. BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE). J Immunol. 1963 Jul;91:112–122. [PubMed] [Google Scholar]
  28. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., MacLeod R. Classification of rhabdovirus proteins: a proposal. J Virol. 1972 Dec;10(6):1228–1230. doi: 10.1128/jvi.10.6.1228-1230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wong P. K., Holloway A. F., Cormack D. V. Characterization of three complementation groups of vesicular stomatitis virus. Virology. 1972 Dec;50(3):829–840. doi: 10.1016/0042-6822(72)90437-0. [DOI] [PubMed] [Google Scholar]

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