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. 1977 Apr;22(1):1–8. doi: 10.1128/jvi.22.1.1-8.1977

Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

J Luka, W Siegert, G Klein
PMCID: PMC515679  PMID: 192908

Abstract

The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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