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. 1977 May;22(2):267–272. doi: 10.1128/jvi.22.2.267-272.1977

Cell cycle dependency of murine cytomegalovirus replication in synchronized 3T3 cells.

M T Muller, J B Hudson
PMCID: PMC515715  PMID: 194054

Abstract

Synchronized murine 3T3 cells have been used to investigate the possible dependency of murine cytomegalovirus replication upon the cell cycle. The normal latent period of 12 h characteristic of asynchronous 3T3 cells was protracted to more than 24 h after an early G1 infection in synchronous cells. In this case viral progeny were not detected until after the initiation of the host S-phase. Cells maintained in the G1 phase did not replicate virus. This failure could not be explained by a decrease in virus penetration but was apparently due to a requirement for an event associated with the host S-phase. Thymidine-induced inhibition of cell cycle traverse also blocked virus replication. Viral DNA synthesis did not initiate until after the initiation of host DNA. In contrast, herpes simplex virus type 1 replicated in 3T3 cells independently of the cell cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOOTSMA D., BUDKE L., VOS O. STUDIES ON SYNCHRONOUS DIVISION OF TISSUE CULTURE CELLS INITIATED BY EXCESS THYMIDINE. Exp Cell Res. 1964 Jan;33:301–309. doi: 10.1016/s0014-4827(64)81035-1. [DOI] [PubMed] [Google Scholar]
  2. Bray G., Brent T. P. Deoxyribonucleoside 5'-triphosphate pool fluctuations during the mammalian cell cycle. Biochim Biophys Acta. 1972 May 10;269(2):184–191. doi: 10.1016/0005-2787(72)90425-x. [DOI] [PubMed] [Google Scholar]
  3. Cohen G. H., Factor M. N., Ponce de Leon M. Inhibition of herpes simplex virus type 2 replication by thymidine. J Virol. 1974 Jul;14(1):20–25. doi: 10.1128/jvi.14.1.20-25.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeMarchi J. M., Kaplan A. S. Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. J Virol. 1976 Jun;18(3):1063–1070. doi: 10.1128/jvi.18.3.1063-1070.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hampar B., Lenoir G., Nonoyama M., Derger J. G., Chang S. Cell cycle dependence for activation of Epstein-Barr virus by inhibitors of protein synthesis or medium deficient in arginine. Virology. 1976 Feb;69(2):660–668. doi: 10.1016/0042-6822(76)90494-3. [DOI] [PubMed] [Google Scholar]
  6. Henson D., Smith R. D., Gehrke J. Murine cytomegalovirus: observations on growth in vitro, cytopathic effect, and inhibition with 5-Iododeoxyuridine. Arch Gesamte Virusforsch. 1966;18(4):433–444. doi: 10.1007/BF01246575. [DOI] [PubMed] [Google Scholar]
  7. Holley R. W., Kiernan J. A. "Contact inhibition" of cell division in 3T3 cells. Proc Natl Acad Sci U S A. 1968 May;60(1):300–304. doi: 10.1073/pnas.60.1.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hudson J. B., Misra V., Mosmann T. R. Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity. Virology. 1976 Jul 1;72(1):235–243. doi: 10.1016/0042-6822(76)90326-3. [DOI] [PubMed] [Google Scholar]
  9. Hudson J. B., Misra V., Mosmann T. R. Properties of the multicapsid virions of murine cytomegalovirus. Virology. 1976 Jul 1;72(1):224–234. doi: 10.1016/0042-6822(76)90325-1. [DOI] [PubMed] [Google Scholar]
  10. Kaplan J. C., Kleinman L. F., Black P. H. Cell cycle dependence of simian virus 40 induction from transformed hamster cells by ultraviolet irradiation. Virology. 1975 Nov;68(1):215–220. doi: 10.1016/0042-6822(75)90162-2. [DOI] [PubMed] [Google Scholar]
  11. Lawrence W. C. Evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the S phase of the KB cell mitotic cycle. J Virol. 1971 Jun;7(6):736–748. doi: 10.1128/jvi.7.6.736-748.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mosmann T. R., Hudson J. B. Some properties of the genome of murine cytomegalovirus (MCV). Virology. 1973 Jul;54(1):135–149. doi: 10.1016/0042-6822(73)90123-2. [DOI] [PubMed] [Google Scholar]
  13. Mosmann T. R., Hudson J. B. Structural and functional heterogeneity of the murine cytomegalovirus genome. Virology. 1974 Nov;62(1):175–183. doi: 10.1016/0042-6822(74)90313-4. [DOI] [PubMed] [Google Scholar]
  14. Osborn J. E., Walker D. L. Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation. J Virol. 1968 Sep;2(9):853–858. doi: 10.1128/jvi.2.9.853-858.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roller B., Cohen G. H. Deoxyribonucleoside triphosphate pools in synchronized human cells infected with herpes simplex virus types 1 and 2. J Virol. 1976 Apr;18(1):58–64. doi: 10.1128/jvi.18.1.58-64.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Skoog K. L., Nordenskjöld B. A., Bjursell K. G. Deoxyribonucleoside-triphosphate pools and DNA synthesis in synchronized hamster cells. Eur J Biochem. 1973 Mar 15;33(3):428–432. doi: 10.1111/j.1432-1033.1973.tb02699.x. [DOI] [PubMed] [Google Scholar]
  17. Thorne H. V. Cyclic variation in susceptibility of Balb-c 3T3 cells to polyoma virus. J Gen Virol. 1973 Feb;18(2):163–169. doi: 10.1099/0022-1317-18-2-163. [DOI] [PubMed] [Google Scholar]
  18. Weber M. J., Edlin G. Phosphate transport, nucleotide pools, and ribonucleic acid synthesis in growing and in density-inhibited 3T3 cells. J Biol Chem. 1971 Mar 25;246(6):1828–1833. [PubMed] [Google Scholar]
  19. XEROS N. Deoxyriboside control and synchronization of mitosis. Nature. 1962 May 19;194:682–683. doi: 10.1038/194682a0. [DOI] [PubMed] [Google Scholar]

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