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. 1977 Jul;23(1):117–125. doi: 10.1128/jvi.23.1.117-125.1977

DNA polymerase activities in growing cells infected with simian virus 40.

M Méchali, M Girard, A M de Recondo
PMCID: PMC515807  PMID: 69720

Abstract

Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity of DNA polymerase-alpha increased five- to sixfold in the nuclear Triton wash and threefold in the nuclei and then remained enhanced only inside the nuclei. That of DNA polymerases-beta and gamma increased mostly in the nuclei after infection. These results suggest that DNA polymerase-alpha could be the major enzyme involved in SV40 DNA replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson R. P., Blobel G. On the attachment of the nuclear pore complex. J Cell Biol. 1974 Sep;62(3):746–754. doi: 10.1083/jcb.62.3.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baril E. F., Jenkins M. D., Brown O. E., Laszlo J., Morris H. P. DNA polymerases I and II in regenerating rat liver and Morris hepatomas. Cancer Res. 1973 Jun;33(6):1187–1193. [PubMed] [Google Scholar]
  4. Bollum F. J. Mammalian DNA polymerases. Prog Nucleic Acid Res Mol Biol. 1975;15(0):109–144. doi: 10.1016/s0079-6603(08)60118-x. [DOI] [PubMed] [Google Scholar]
  5. Chang L. M., Brown M., Bollum F. J. Induction of DNA polymerase in mouse L cells. J Mol Biol. 1973 Feb 15;74(1):1–8. doi: 10.1016/0022-2836(73)90349-5. [DOI] [PubMed] [Google Scholar]
  6. Chang L. M. Replication of initiated polyriboadenylic acid by mammalian low molecular weight deoxyribonucleic acid polymerase. J Biol Chem. 1974 Dec 10;249(23):7441–7446. [PubMed] [Google Scholar]
  7. Hatanaka M., Dulbecco R. Induction of DNA synthesis by SV40. Proc Natl Acad Sci U S A. 1966 Aug;56(2):736–740. doi: 10.1073/pnas.56.2.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kit S., De Torres R. A., Dubbs D. R., Salvi M. L. Induction of cellular deoxyribonuleic acid synthesis by simian virus 40. J Virol. 1967 Aug;1(4):738–746. doi: 10.1128/jvi.1.4.738-746.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kit S., Dubbs D. R., Frearson P. M. Enzymes of nucleic acid metabolism in cells infected with polyoma virus. Cancer Res. 1966 Apr;26(4):638–646. [PubMed] [Google Scholar]
  10. Knopf K. W., Yamada M., Weissbach A. HeLa cell DNA polymerase gamma: further purification and properties of the enzyme. Biochemistry. 1976 Oct 5;15(20):4540–4548. doi: 10.1021/bi00665a032. [DOI] [PubMed] [Google Scholar]
  11. LeBlanc D. J., Singer M. F. Localization of replicating DNA of simian virus 40 in monkey kidney cells. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2236–2240. doi: 10.1073/pnas.71.6.2236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loeb L. A. Purification and properties of deoxyribonucleic acid polymerase from nuclei of sea urchin embryos. J Biol Chem. 1969 Apr 10;244(7):1672–1681. [PubMed] [Google Scholar]
  13. Magnusson G., Pigiet V., Winnacker E. L., Abrams R., Reichard P. RNA-linked short DNA fragments during polyoma replication. Proc Natl Acad Sci U S A. 1973 Feb;70(2):412–415. doi: 10.1073/pnas.70.2.412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Magnusson G., Winnacker E. L., Eliasson R., Reichard P. Replication of polyoma DNA in isolated nuclei. II. Evidence for semi-conservative replication. J Mol Biol. 1972 Dec 30;72(3):539–552. doi: 10.1016/0022-2836(72)90173-8. [DOI] [PubMed] [Google Scholar]
  15. Manteuil S., Pages J., Stehelin D., Girard M. Replication of simian virus 40 deoxyribonucleic acid: analysis of the one-step growth cycle. J Virol. 1973 Jan;11(1):98–106. doi: 10.1128/jvi.11.1.98-106.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Matsukage A., Bohn E. W., Wilson S. H. On the DNA polymerase III of mouse myeloma: partial purification and characterization. Biochemistry. 1975 Mar 11;14(5):1006–1020. doi: 10.1021/bi00676a020. [DOI] [PubMed] [Google Scholar]
  17. Mechali M., de Recondo A. M. Co-fractionation of an endonuclease activity during the purification of DNA polymerase-alpha from regenerating rat liver. Properties and separation from DNA polymerase. Eur J Biochem. 1975 Oct 15;58(2):461–466. doi: 10.1111/j.1432-1033.1975.tb02393.x. [DOI] [PubMed] [Google Scholar]
  18. Närkhammar M., Magnusson G. DNA polymerase activities induced by polyoma virus infection of 3T3 mouse fibroblasts. J Virol. 1976 Apr;18(1):1–6. doi: 10.1128/jvi.18.1.1-6.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pages J., Manteuil S., Stehelin D., Fiszman M., Marx M., Girard M. Relationship between replication of simian virus 40 DNA and specific events of the host cell cycle. J Virol. 1973 Jul;12(1):99–107. doi: 10.1128/jvi.12.1.99-107.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Salzman N. P., Fareed G. C., Sebring E. D., Thoren M. M. The mechanism of SV40 DNA replication. Cold Spring Harb Symp Quant Biol. 1974;38:257–265. doi: 10.1101/sqb.1974.038.01.029. [DOI] [PubMed] [Google Scholar]
  21. Sauer G., Fischer H., Munk K. The effect of SV40 infection on DNA synthesis in cercopithecus kidney cells. Virology. 1966 Apr;28(4):765–767. doi: 10.1016/0042-6822(66)90264-9. [DOI] [PubMed] [Google Scholar]
  22. Sheinin R. Deoxyribonucleic acid synthesis in cells replicating polyoma virus. Virology. 1966 Apr;28(4):621–632. doi: 10.1016/0042-6822(66)90247-9. [DOI] [PubMed] [Google Scholar]
  23. Spadari S., Weissbach A. RNA-primed DNA synthesis: specific catalysis by HeLa cell DNA polymerase alpha. Proc Natl Acad Sci U S A. 1975 Feb;72(2):503–507. doi: 10.1073/pnas.72.2.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spadari S., Weissbach A. The interrelation between DNA synthesis and various DNA polymerase activities in synchronized HeLa cells. J Mol Biol. 1974 Jun 15;86(1):11–20. doi: 10.1016/s0022-2836(74)80003-3. [DOI] [PubMed] [Google Scholar]
  25. WASSERMAN E., LEVINE L. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961 Sep;87:290–295. [PubMed] [Google Scholar]
  26. Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]
  27. Weissbach A. Vertebrate DNA polymerases. Cell. 1975 Jun;5(2):101–108. doi: 10.1016/0092-8674(75)90017-3. [DOI] [PubMed] [Google Scholar]
  28. Wintersberger U., Wintersberger E. DNA polymerases in polyoma virus-infected mouse kidney cells. J Virol. 1975 Nov;16(5):1095–1100. doi: 10.1128/jvi.16.5.1095-1100.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. de Recondo A. M., Abadiedebat J. Regenerating rat liver DNA polymerases: disimilitude or relationship between nuclear and cytoplasmic enzymes? Nucleic Acids Res. 1976 Aug;3(8):1823–1837. doi: 10.1093/nar/3.8.1823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. de Recondo A. M., Lepesant J. A., Fichot O., Grasset L., Rossignol J. M., Cazillis M. Synthetic template specificity of a deoxyribonucleic acid polymerase from regenerating rat liver. J Biol Chem. 1973 Jan 10;248(1):131–137. [PubMed] [Google Scholar]

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