Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 Sep;23(3):599–607. doi: 10.1128/jvi.23.3.599-607.1977

Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins.

A S Khan, J R Stephenson
PMCID: PMC515871  PMID: 197262

Abstract

The major non-glycosylated structural proteins of feline leukemia virus have been isolated, and competition immunoassays have been developed for each. These proteins include the 27,000- to 30,000-molecular-weight major internal antigen designated p30, a 15,000-molecular-weight protein (p15), an acidic protein of 12,000 molecular weight (p12), and a highly basic 10,000-molecular-weight protein (p10). Immunologically and biochemically corresponding proteins of feline and murine leukemia viruses have been identified. and, on the basis of analogy to the known sequence of a prototype type C virus of mouse origin, the map order of the gag region of the feline type C viral genome has been tentatively deduced as NH2-p15-p12-p10-COOH. The demonstration of two feline leukemia virus gag gene-coded proteins, p15 and p12, expressed in the form of an uncleaved precursor in a mink cell line nonproductively transformed by feline sarcoma virus provides indirect support for the proposed sequence.

Full text

PDF
600

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbacid M., Stephenson J. R., Aaronson S. A. Evolutionary relationships between gag gene-coded proteins of murine and primate endogenous type C RNA viruses. Cell. 1977 Apr;10(4):641–648. doi: 10.1016/0092-8674(77)90097-6. [DOI] [PubMed] [Google Scholar]
  2. Barbacid M., Stephenson J. R., Aaronson S. A. gag Gene of mammalian type-C RNA tumour viruses. Nature. 1976 Aug 12;262(5569):554–559. doi: 10.1038/262554a0. [DOI] [PubMed] [Google Scholar]
  3. Benveniste R. E., Sherr C. J., Todaro G. J. Evolution of type C viral genes: origin of feline leukemia virus. Science. 1975 Nov 28;190(4217):886–888. doi: 10.1126/science.52892. [DOI] [PubMed] [Google Scholar]
  4. Benveniste R. E., Todaro G. J. Evolution of C-type viral genes: inheritance of exogenously acquired viral genes. Nature. 1974 Dec 6;252(5483):456–459. doi: 10.1038/252456a0. [DOI] [PubMed] [Google Scholar]
  5. Devare S. G., Stephenson J. R. Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. J Virol. 1977 Aug;23(2):443–447. doi: 10.1128/jvi.23.2.443-447.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gardner M. B., Brown J. C., Charman H. P., Stephenson J. R., Rongey R. W., Hauser D. E., Diegmann F., Howard E., Dworsky R., Gilden R. V. FeLV epidemiology in Los Angeles cats: Appraisal of detection methods. Int J Cancer. 1977 Apr 15;19(4):581–589. doi: 10.1002/ijc.2910190420. [DOI] [PubMed] [Google Scholar]
  8. Graves D. C., Velicer L. F. Properties of feline leukemia virus. I. Chromatographic separation and analysis of the polypeptides. J Virol. 1974 Aug;14(2):349–365. doi: 10.1128/jvi.14.2.349-365.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hardy W. D., Jr, Old L. J., Hess P. W., Essex M., Cotter S. Horizontal transmission of feline leukaemia virus. Nature. 1973 Aug 3;244(5414):266–269. doi: 10.1038/244266a0. [DOI] [PubMed] [Google Scholar]
  10. Henderson I. C., Lieber M. M., Todaro G. J. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology. 1974 Jul;60(1):282–287. doi: 10.1016/0042-6822(74)90386-9. [DOI] [PubMed] [Google Scholar]
  11. JARRETT W. F., MARTIN W. B., CRIGHTON G. W., DALTON R. G., STEWART M. F. TRANSMISSION EXPERIMENTS WITH LEUKEMIA (LYMPHOSARCOMA). Nature. 1964 May 9;202:566–567. doi: 10.1038/202566a0. [DOI] [PubMed] [Google Scholar]
  12. Jamjoom G., Karshin W. L., Naso R. B., Arcement L. J., Arlinghaus R. B. Proteins of Rauscher murine leukemia virus: resolution of a 70,000-dalton, Nonglycosylated polypeptide containing p30 peptide sequences. Virology. 1975 Nov;68(1):135–145. doi: 10.1016/0042-6822(75)90155-5. [DOI] [PubMed] [Google Scholar]
  13. Jarrett W., Jarrett O., Mackey L., Laird H., Hardy W., Jr, Essex M. Horizontal transmission of leukemia virus and leukemia in the cat. J Natl Cancer Inst. 1973 Sep;51(3):833–841. doi: 10.1093/jnci/51.3.833. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V. C-type virus released from cultured human rhabdomyosarcoma cells. Nat New Biol. 1972 Jan 5;235(53):3–6. doi: 10.1038/newbio235003a0. [DOI] [PubMed] [Google Scholar]
  17. Niman H. L., Gardner M. B., Stephenson J. R., Roy-Burman P. Endogenous RD-114 virus genome expression in malignant tissues of domestic cats. J Virol. 1977 Sep;23(3):578–586. doi: 10.1128/jvi.23.3.578-586.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Niman H. L., Stephenson J. R., Gardner M. B., Roy-Burman P. RD-114 and feline leukaemia virus genome expression in natural lymphomas of domestic cats. Nature. 1977 Mar 24;266(5600):357–360. doi: 10.1038/266357a0. [DOI] [PubMed] [Google Scholar]
  19. Okasinski G. F., Velicer L. F. Analysis of intracellular feline leukemia virus proteins II. Generation of feline leukemia virus structural proteins from precursor polypeptides. J Virol. 1977 Apr;22(1):74–85. doi: 10.1128/jvi.22.1.74-85.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oroszlan S., Huebner R. J., Gilden R. V. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus. Proc Natl Acad Sci U S A. 1971 May;68(5):901–904. doi: 10.1073/pnas.68.5.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  22. Scolnick E. M., Parks W. P., Livingston D. M. Radioimmunoassay of mammalian type C viral proteins. I. Species specific reactions of murine and feline viruses. J Immunol. 1972 Sep;109(3):570–577. [PubMed] [Google Scholar]
  23. Stephenson J. R., Essex M., Hino S., Hardy W. D., Jr, Aaronson S. A. Feline oncornavirus-associated cell-membrane antigen (FOCMA): distinction between FOCMA and the major virion glycoprotein. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1219–1223. doi: 10.1073/pnas.74.3.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stephenson J. R., Hino S., Garrett E. W., Aaronson S. A. Immunological cross reactivity of Mason-Pfizer monkey virus with type C RNA viruses endogenous to primates. Nature. 1976 Jun 17;261(5561):609–611. doi: 10.1038/261609a0. [DOI] [PubMed] [Google Scholar]
  25. Stephenson J. R., Tronick S. R., Aaronson S. A. Analysis of type specific antigenic determinants of two structural polypeptides of mouse RNA C-Type viruses. Virology. 1974 Mar;58(1):1–8. doi: 10.1016/0042-6822(74)90135-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES