Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 Oct;24(1):91–98. doi: 10.1128/jvi.24.1.91-98.1977

Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses.

S M Rodger, R D Schnagl, I H Holmes
PMCID: PMC515913  PMID: 198589

Abstract

Polyacrylamide gel electrophoretic analysis of purified preparation of the simian rotavirus SA-11 indicated eight polypeptide components that migrated in a manner remarkably similar to those of the previously characterized human and calf rotaviruses. Analyses of preparations of single-shelled and double-shelled particles of human, calf, and simian an rotaviruses have also permitted assignment of the polypeptides to the inner or outer shells. The major components of the outer shells of each virus have been identified as glycoproteins, and the importance of this in terms of host cell specificity is discussed. Sensitivities of the various rotaviruses to acid, proteases, and glycosidases were also investigated.

Full text

PDF
94

Images in this article

93Image
on p.93
95Image
on p.95
95Image
on p.95
95Image
on p.95

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  2. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  3. Borsa J., Copps T. P., Sargent M. D., Long D. G., Chapman J. D. New intermediate subviral particles in the in vitro uncoating of reovirus virions by chymotrypsin. J Virol. 1973 Apr;11(4):552–564. doi: 10.1128/jvi.11.4.552-564.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bridger J. C., Woode G. N. Characterization of two particle types of calf rotavirus. J Gen Virol. 1976 May;31(2):245–250. doi: 10.1099/0022-1317-31-2-245. [DOI] [PubMed] [Google Scholar]
  5. Cardiff R. D., Puentes M. J., Teramoto Y. A., Lund J. K. Structure of the mouse mammary tumor virus: characterization of bald particles. J Virol. 1974 Nov;14(5):1293–1303. doi: 10.1128/jvi.14.5.1293-1303.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Compans R. W., Klenk H. D., Caliguiri L. A., Choppin P. W. Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology. 1970 Dec;42(4):880–889. doi: 10.1016/0042-6822(70)90337-5. [DOI] [PubMed] [Google Scholar]
  7. Cross R. K., Fields B. N. Reovirus-specific polypeptides: analysis using discontinuous gel electrophoresis. J Virol. 1976 Jul;19(1):162–173. doi: 10.1128/jvi.19.1.162-173.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Editorial: Rotaviruses of man and animals. Lancet. 1975 Feb 1;1(7901):257–258. [PubMed] [Google Scholar]
  10. Els H. J., Lecatsas G. Morphological studies on simian virus S.A. 11 and the 'related' O agent. J Gen Virol. 1972 Oct;17(1):129–132. doi: 10.1099/0022-1317-17-1-129. [DOI] [PubMed] [Google Scholar]
  11. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974 Jul 13;2(7872):61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  12. Glossmann H., Neville D. M., Jr Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat. J Biol Chem. 1971 Oct 25;246(20):6339–6346. [PubMed] [Google Scholar]
  13. Holmes I. H., Rodger S. M., Schnagl R. D., Ruck B. J., Gust I. D., Bishop R. F., Barnes G. L. Is lactase the receptor and uncoating enzyme for infantile enteritis (rota) viruses? Lancet. 1976 Jun 26;1(7974):1387–1388. doi: 10.1016/s0140-6736(76)93032-4. [DOI] [PubMed] [Google Scholar]
  14. Infantile enteritis viruses: morphogenesis and morphology. J Virol. 1975 Oct;16(4):937–943. doi: 10.1128/jvi.16.4.937-943.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ishibashi M., Maizel J. V., Jr The polypeptides of adenovirus. VI. Early and late glycopolypeptides. Virology. 1974 Apr;58(2):345–361. doi: 10.1016/0042-6822(74)90070-1. [DOI] [PubMed] [Google Scholar]
  16. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., van Kirk D. H., Chanock R. M., Kapikian A. Z. Differentiation of human and calf reoviruslike agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. Virology. 1976 Oct 1;74(1):86–92. doi: 10.1016/0042-6822(76)90131-8. [DOI] [PubMed] [Google Scholar]
  17. Kapikian A. Z., Cline W. L., Kim H. W., Kalica A. R., Wyatt R. G., Vankirk D. H., Chanock R. M., James H. D., Jr, Vaughn A. L. Antigenic relationships among five reovirus-like (RVL) agents by complement fixation (CF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis. Proc Soc Exp Biol Med. 1976 Sep;152(4):535–539. doi: 10.3181/00379727-152-39434. [DOI] [PubMed] [Google Scholar]
  18. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Jr, VanKirk D., Chanock R. M. New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. Lancet. 1975 May 10;1(7915):1056–1061. doi: 10.1016/s0140-6736(75)91827-9. [DOI] [PubMed] [Google Scholar]
  19. Krystal G., Perrault J., Graham A. F. Evidence for a glycoprotein in reovirus. Virology. 1976 Jul 15;72(2):308–321. doi: 10.1016/0042-6822(76)90160-4. [DOI] [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. Lerner A. M., Bailey E. J., Tillotson J. R. Enterovirus hemagglutination: inhibition by several enzymes and sugars. J Immunol. 1965 Dec;95(6):1111–1115. [PubMed] [Google Scholar]
  22. Lerner A. M., Miranda Q. R. Cellular interactions of several enteroviruses and a reovirus after treatment with sodium borohydride or carbohydrases. Virology. 1968 Oct;36(2):277–285. doi: 10.1016/0042-6822(68)90145-1. [DOI] [PubMed] [Google Scholar]
  23. Malherbe H. H., Strickland-Cholmley M. Simian virus SA11 and the related O agent. Arch Gesamte Virusforsch. 1967;22(1):235–245. doi: 10.1007/BF01240518. [DOI] [PubMed] [Google Scholar]
  24. Newman J. F., Brown F., Bridger J. C., Woode G. N. Characterisation of a rotavirus.20b. Nature. 1975 Dec 18;258(5536):631–633. doi: 10.1038/258631a0. [DOI] [PubMed] [Google Scholar]
  25. Obijeski J. F., Palmer E. L., Martin M. L. Biochemical characterization of infantile gastroenteritis virus (IGV). J Gen Virol. 1977 Mar;34(3):485–497. doi: 10.1099/0022-1317-34-3-485. [DOI] [PubMed] [Google Scholar]
  26. Rodger S. M., Schnagl R. D., Holmes I. H. Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin. J Virol. 1975 Nov;16(5):1229–1235. doi: 10.1128/jvi.16.5.1229-1235.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schnagl R. D., Holmes I. H. Characteristics of the genome of human infantile enteritis virus (Rotavirus). J Virol. 1976 Jul;19(1):267–270. doi: 10.1128/jvi.19.1.267-270.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. TYRRELL D. A., CHANOCK R. M. Rhinoviruses: a description. Science. 1963 Jul 12;141(3576):152–153. doi: 10.1126/science.141.3576.152. [DOI] [PubMed] [Google Scholar]
  29. Welch A. B., Twiehaus M. J. Cell culture studies of a neonatal calf diarrhea virus. Can J Comp Med. 1973 Jul;37(3):287–294. [PMC free article] [PubMed] [Google Scholar]
  30. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Davies H. A., Davis H. A., White G. B. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. Infect Immun. 1976 Sep;14(3):804–810. doi: 10.1128/iai.14.3.804-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES