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. 1977 Oct;24(1):201–210. doi: 10.1128/jvi.24.1.201-210.1977

Properties of "diplophage": a lipid-containing bacteriophage.

R Lopez, C Ronda, A Tomasz, A Portoles
PMCID: PMC515923  PMID: 20516

Abstract

We describe the purification and properties of Dp-1, a bacteriophage isolated from Diplococcus pneumoniae. The phage was sensitive to the organic solvents deoxycholate and Sarkosyl, and its infectivity was reduced by treatment with phospholipase C. Electron microscopy indicated the presence of a double-layered coat around the phage particles. Purified phage preparations contained lipid amounting to about 8.5% of the dry weight of the phage, and thin-layer chromatography resolved the lipids into four components. The phage had a buoyant density in CsCl of 1.47 g/cm3, and a sedimentation constant in 0.1 M NaCl of 313S. Analysis in acrylamide gel electrophoresis indicated the presence of three major proteins. Dp-1 DNA shows a density of 1.681 g/cm3. Neutralizing antisera against the phage have a low potency (K less than 120/min).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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