Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 Dec;24(3):768–774. doi: 10.1128/jvi.24.3.768-774.1977

DNA replication in bacteriophage-infected Staphylococcus aureus.

M H Ubelaker, E D Rosenblum
PMCID: PMC515998  PMID: 145499

Abstract

The synthesis of viral and host DNA in phage-infected Staphylococcus aureus was examined. Three intracellular forms of phage 52HJD DNA were demonstrated: covalently closed circular, open circular, and linear DNA species. It was noted that infection of S. aureus-propagating strains 81 and 52 with phage 52HJD inhibited the replication of the bacterial chromosome and a stringently controlled penicillinase plasmid. A small tetracycline plasmid, normally under relaxed replication control, continued to replicate in the postinfection period. No breakdown of the host chromosome into small-molecular-weight fragments or utilization of bacterial DNA material for the synthesis of viral DNA was observed.

Full text

PDF
770

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLAIR J. E., BYNOE E. T. Variation in three staphylococcal typing phages. Public Health Rep. 1958 May;73(5):465–466. [PMC free article] [PubMed] [Google Scholar]
  2. BLAIR J. E., CARR M. The bacteriophage typing of staphylococci. J Infect Dis. 1953 Jul-Aug;93(1):1–13. doi: 10.1093/infdis/93.1.1. [DOI] [PubMed] [Google Scholar]
  3. Bernstein H., Bernstein C. Circular and branched circular concatenates as possible intermediates in bacteriophage T4 DNA replication. J Mol Biol. 1973 Jul 5;77(3):355–361. doi: 10.1016/0022-2836(73)90443-9. [DOI] [PubMed] [Google Scholar]
  4. Botstein D., Levine M. Intermediates in the synthesis of phage P22 DNA. Cold Spring Harb Symp Quant Biol. 1968;33:659–667. doi: 10.1101/sqb.1968.033.01.075. [DOI] [PubMed] [Google Scholar]
  5. Botstein D., Matz M. J. A recombination function essential to the growth of bacteriophage P22. J Mol Biol. 1970 Dec 28;54(3):417–440. doi: 10.1016/0022-2836(70)90119-1. [DOI] [PubMed] [Google Scholar]
  6. Botstein D. Synthesis and maturation of phage P22 DNA. I. Identification of intermediates. J Mol Biol. 1968 Jun 28;34(3):621–641. doi: 10.1016/0022-2836(68)90185-x. [DOI] [PubMed] [Google Scholar]
  7. Botstein D., Waddell C. H., King J. Mechanism of head assembly and DNA encapsulation in Salmonella phage p22. I. Genes, proteins, structures and DNA maturation. J Mol Biol. 1973 Nov 15;80(4):669–695. doi: 10.1016/0022-2836(73)90204-0. [DOI] [PubMed] [Google Scholar]
  8. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
  10. Frankel F. R. DNA replication after T4 infection. Cold Spring Harb Symp Quant Biol. 1968;33:485–493. doi: 10.1101/sqb.1968.033.01.056. [DOI] [PubMed] [Google Scholar]
  11. Frankel F. R. Studies on the nature of replicating DNA in T4-infected Escherichia coli. J Mol Biol. 1966 Jun;18(1):127–143. doi: 10.1016/s0022-2836(66)80081-5. [DOI] [PubMed] [Google Scholar]
  12. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  13. Ikeda H., Tomizawa J. I. Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragments. J Mol Biol. 1965 Nov;14(1):85–109. doi: 10.1016/s0022-2836(65)80232-7. [DOI] [PubMed] [Google Scholar]
  14. Jaenisch R., Mayer A., Levine A. Replicating SV40 molecules containing closed circular template DNA strands. Nat New Biol. 1971 Sep 15;233(37):72–75. doi: 10.1038/newbio233072a0. [DOI] [PubMed] [Google Scholar]
  15. Koerner J. F. Enzymes of nucleic acid metabolism. Annu Rev Biochem. 1970;39:291–322. doi: 10.1146/annurev.bi.39.070170.001451. [DOI] [PubMed] [Google Scholar]
  16. McClure S. C., Gold M. Bacteriophage lambda DNA synthesized by mutants in gene N. Virology. 1973 Jul;54(1):283–286. doi: 10.1016/0042-6822(73)90138-4. [DOI] [PubMed] [Google Scholar]
  17. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  18. Notani N. K., Setlow J. K., Allison D. P. Intracellular events during infection by Haemophilus influenzae phage and transfection by its DNA. J Mol Biol. 1973 Apr 25;75(4):581–599. doi: 10.1016/0022-2836(73)90293-3. [DOI] [PubMed] [Google Scholar]
  19. Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
  20. Ogawa T., Tomizawa J. I., Fuke M. Replication of bacteriophage DNA, II. Structure of replicating DNA of phage lambda. Proc Natl Acad Sci U S A. 1968 Jul;60(3):861–865. doi: 10.1073/pnas.60.3.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pariza M. W., Iandolo J. J. Determination of genome size of selected typing bacteriophages of Staphylococcus aureus. Appl Microbiol. 1974 Sep;28(3):510–512. doi: 10.1128/am.28.3.510-512.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Robberson D. L., Kasamatsu H., Vinograd J. Replication of mitochondrial DNA. Circular replicative intermediates in mouse L cells. Proc Natl Acad Sci U S A. 1972 Mar;69(3):737–741. doi: 10.1073/pnas.69.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rubin S. J., Rosenblum E. D. Effects of the recipient strain and ultraviolet irradiation on transduction kinetics of the penicillinase plasmid of Staphylococcus aureus. J Bacteriol. 1971 Dec;108(3):1192–1199. doi: 10.1128/jb.108.3.1192-1199.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ruby C., Novick R. P. Plasmid interactions in Staphylococcus aureus: nonadditivity of compatible plasmid DNA pools. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5031–5035. doi: 10.1073/pnas.72.12.5031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  26. Salzman L. A., Weissbach A. Formation of intermediates in the replication of phage lambda DNA. J Mol Biol. 1967 Aug 28;28(1):53–70. doi: 10.1016/s0022-2836(67)80077-9. [DOI] [PubMed] [Google Scholar]
  27. Schmieger H. The fate of the bacterial chromosome in P22-infected cells of Salmonella typhimurium. Mol Gen Genet. 1971;110(3):238–244. doi: 10.1007/BF00337836. [DOI] [PubMed] [Google Scholar]
  28. Schnös M., Inman R. B. Position of branch points in replicating lambda DNA. J Mol Biol. 1970 Jul 14;51(1):61–73. doi: 10.1016/0022-2836(70)90270-6. [DOI] [PubMed] [Google Scholar]
  29. Sheehy R. J., Novick R. P. Studies on plasmid replication. V Replicative intermediates. J Mol Biol. 1975 Apr 5;93(2):237–253. doi: 10.1016/0022-2836(75)90130-8. [DOI] [PubMed] [Google Scholar]
  30. Smith M. G., Skalka A. Some properties of DNA from phage-infected bacteria. J Gen Physiol. 1966 Jul;49(6):127–142. doi: 10.1085/jgp.49.6.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Thomas C. A., Jr, Kelly T. J., Jr, Rhoades M. The intracellular forms of T7 and P22 DNA molecules. Cold Spring Harb Symp Quant Biol. 1968;33:417–424. doi: 10.1101/sqb.1968.033.01.048. [DOI] [PubMed] [Google Scholar]
  32. Wake R. G., Kaiser A. D., Inman R. B. Isolation and structure of phage lambda head-mutant DNA. J Mol Biol. 1972 Mar 14;64(3):519–540. doi: 10.1016/0022-2836(72)90080-0. [DOI] [PubMed] [Google Scholar]
  33. YOUNG E. T., 2nd, SINSHEIMER R. L. NOVEL INTRA-CELLULAR FORMS OF LAMBDA DNA. J Mol Biol. 1964 Dec;10:562–564. doi: 10.1016/s0022-2836(64)80080-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES