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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Jun 1;88(11):4835–4839. doi: 10.1073/pnas.88.11.4835

Deficiency of essential fatty acids and membrane fluidity during pregnancy and lactation.

R T Holman 1, S B Johnson 1, P L Ogburn 1
PMCID: PMC51761  PMID: 2052562

Abstract

In a group of 19 normal pregnant women, plasma lipids were extracted, phospholipids were isolated, and the fatty acid (FA) compositions were measured by capillary gas chromatography. Blood samples were taken at 36 wk, at labor, and at 6 wk postpartum. The FA profiles showed deficiencies of omega 6 and omega 3 FA (omega indicating the length of the terminal saturated chain), the latter more severe, at all three times. Mean melting point (MMP) was calculated for each sample as an index of "fluidity" based upon all FA present. MMP varied linearly with total polyunsaturated FA and with double bond index, current measures of "fluidity" and essential FA status. MMP was elevated 9-11 degrees C in plasma phospholipids of women during pregnancy and labor and postpartum. Lactating mothers showed less recovery from the deficiencies than did the nonlactating mothers, but neither approached normal at 6 wk. The changes seen in phospholipid profiles suggest a significant transfer of omega 3 and omega 6 polyunsaturated FA from the mother to the fetus. These FA are essential for normal fetal growth and development; their relative deficiency in maternal circulation suggests that dietary supplementation may be indicated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alden P. B., Svingen B. A., Deutschman C. S., Johnson S. B., Konstantinides F. N., Holman R. T., Cerra F. B. Essential fatty acid status in isolated closed head injury. J Trauma. 1987 Sep;27(9):1039–1043. doi: 10.1097/00005373-198709000-00014. [DOI] [PubMed] [Google Scholar]
  2. Alden P. B., Svingen B. A., Johnson S. B., Konstantinides F. N., Holman R. T., Cerra F. B. Partial correction by exogenous lipid of abnormal patterns of polyunsaturated fatty acids in plasma phospholipids of stressed and septic surgical patients. Surgery. 1986 Oct;100(4):671–678. [PubMed] [Google Scholar]
  3. Cerra F. B., Alden P. A., Negro F., Billiar T., Svingen B. A., Licari J., Johnson S. B., Holman R. T. Sepsis and exogenous lipid modulation. JPEN J Parenter Enteral Nutr. 1988 Nov-Dec;12(6 Suppl):63S–68S. doi: 10.1177/014860718801200606. [DOI] [PubMed] [Google Scholar]
  4. Crawford M. A., Doyle W., Drury P., Lennon A., Costeloe K., Leighfield M. n-6 and n-3 fatty acids during early human development. J Intern Med Suppl. 1989;731:159–169. doi: 10.1111/j.1365-2796.1989.tb01450.x. [DOI] [PubMed] [Google Scholar]
  5. Crawford M. A. Estimation of essential fatty acid requirements in pregnancy and lactation. Prog Food Nutr Sci. 1980;4(5):75–80. [PubMed] [Google Scholar]
  6. Crawford M. A., Hassam A. G., Stevens P. A. Essential fatty acid requirements in pregnancy and lactation with special reference to brain development. Prog Lipid Res. 1981;20:31–40. doi: 10.1016/0163-7827(81)90011-4. [DOI] [PubMed] [Google Scholar]
  7. Dyck P. J., Yao J. K., Knickerbocker D. E., Holman R. T., Gomez M. R., Hayles A. B., Lambert E. H. Multisystem neuronal degeneration, hepatosplenomegaly, and adrenocortical deficiency associated with reduced tissue arachidonic acid. Neurology. 1981 Aug;31(8):925–934. doi: 10.1212/wnl.31.8.925. [DOI] [PubMed] [Google Scholar]
  8. HOLMAN R. T. NUTRITIONAL AND METABOLIC INTERRELATIONSHIPS BETWEEN FATTY ACIDS. Fed Proc. 1964 Sep-Oct;23:1062–1067. [PubMed] [Google Scholar]
  9. HOLMAN R. T. The ratio of trienoic: tetraenoic acids in tissue lipids as a measure of essential fatty acid requirement. J Nutr. 1960 Mar;70:405–410. doi: 10.1093/jn/70.3.405. [DOI] [PubMed] [Google Scholar]
  10. Hernell O., Holmgren G., Jagell S. F., Johnson S. B., Holman R. T. Suspected faulty essential fatty acid metabolism in Sjögren-Larsson syndrome. Pediatr Res. 1982 Jan;16(1):45–49. doi: 10.1203/00006450-198201001-00009. [DOI] [PubMed] [Google Scholar]
  11. Holman R. T., Johnson S. B., Hatch T. F. A case of human linolenic acid deficiency involving neurological abnormalities. Am J Clin Nutr. 1982 Mar;35(3):617–623. doi: 10.1093/ajcn/35.3.617. [DOI] [PubMed] [Google Scholar]
  12. Holman R. T., Johnson S. B., Hatch T. F. A case of human linolenic acid deficiency involving neurological abnormalities. Am J Clin Nutr. 1982 Mar;35(3):617–623. doi: 10.1093/ajcn/35.3.617. [DOI] [PubMed] [Google Scholar]
  13. Holman R. T., Johnson S. B., Kokmen E. Deficiencies of polyunsaturated fatty acids and replacement by nonessential fatty acids in plasma lipids in multiple sclerosis. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4720–4724. doi: 10.1073/pnas.86.12.4720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holman R. T., Johnson S. B., Mercuri O., Itarte H. J., Rodrigo M. A., De Tomas M. E. Essential fatty acid deficiency in malnourished children. Am J Clin Nutr. 1981 Aug;34(8):1534–1539. doi: 10.1093/ajcn/34.8.1534. [DOI] [PubMed] [Google Scholar]
  15. Holman R. T., Johnson S. Changes in essential fatty acid profile of serum phospholipids in human disease. Prog Lipid Res. 1981;20:67–73. doi: 10.1016/0163-7827(81)90015-1. [DOI] [PubMed] [Google Scholar]
  16. Holman R. T., Johnson S. Changes in essential fatty acid profile of serum phospholipids in human disease. Prog Lipid Res. 1981;20:67–73. doi: 10.1016/0163-7827(81)90015-1. [DOI] [PubMed] [Google Scholar]
  17. Johnson S. B., Gordon E., McClain C., Low G., Holman R. T. Abnormal polyunsaturated fatty acid patterns of serum lipids in alcoholism and cirrhosis: arachidonic acid deficiency in cirrhosis. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1815–1818. doi: 10.1073/pnas.82.6.1815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MOHRHAUER H., HOLMAN R. T. ALTERATION OF THE FATTY ACID COMPOSITION OF BRAIN LIPIDS BY VARYING LEVELS OF DIETARY ESSENTIAL FATTY ACIDS. J Neurochem. 1963 Jul;10:523–530. doi: 10.1111/j.1471-4159.1963.tb09855.x. [DOI] [PubMed] [Google Scholar]
  19. MOHRHAUER H., HOLMAN R. T. THE EFFECT OF DIETARY ESSENTIAL FATTY ACIDS UPON COMPOSITION OF POLYUNSATURATED FATTY ACIDS IN DEPOT FAT AND ERYTHROCYTES OF THE RAT. J Lipid Res. 1963 Jul;4:346–350. [PubMed] [Google Scholar]
  20. MOHRHAUER H., HOLMAN R. T. THE EFFECT OF DOSE LEVEL OF ESSENTIAL FATTY ACIDS UPON FATTY ACID COMPOSITION OF THE RAT LIVER. J Lipid Res. 1963 Apr;4:151–159. [PubMed] [Google Scholar]
  21. Mock D. M., Johnson S. B., Holman R. T. Effects of biotin deficiency on serum fatty acid composition: evidence for abnormalities in humans. J Nutr. 1988 Mar;118(3):342–348. doi: 10.1093/jn/118.3.342. [DOI] [PubMed] [Google Scholar]
  22. Ogburn P. L., Jr, Sharp H., Lloyd-Still J. D., Johnson S. B., Holman R. T. Abnormal polyunsaturated fatty acid patterns of serum lipids in Reye's syndrome. Proc Natl Acad Sci U S A. 1982 Feb;79(3):908–911. doi: 10.1073/pnas.79.3.908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ogburn P. L., Jr, Williams P. P., Johnson S. B., Holman R. T. Serum arachidonic acid levels in normal and preeclamptic pregnancies. Am J Obstet Gynecol. 1984 Jan 1;148(1):5–9. doi: 10.1016/s0002-9378(84)80023-x. [DOI] [PubMed] [Google Scholar]
  24. Paulsrud J. R., Pensler L., Whitten C. F., Stewart S., Holman R. T. Essential fatty acid deficiency in infants induced by fat-free intravenous feeding. Am J Clin Nutr. 1972 Sep;25(9):897–904. doi: 10.1093/ajcn/25.9.897. [DOI] [PubMed] [Google Scholar]
  25. Phinney S. D., Odin R. S., Johnson S. B., Holman R. T. Reduced arachidonate in serum phospholipids and cholesteryl esters associated with vegetarian diets in humans. Am J Clin Nutr. 1990 Mar;51(3):385–392. doi: 10.1093/ajcn/51.3.385. [DOI] [PubMed] [Google Scholar]
  26. Sharp H. L., Lindahl J. A., Freese D. K., Burke B., Englund J., Johnson D., Johnson S. B., Holman R. T. A new hepato-pancreato-renal disorder resembling tyrosinemia involving neuropathy and abnormal metabolism of polyunsaturated acids. J Pediatr Gastroenterol Nutr. 1988 Mar-Apr;7(2):167–176. doi: 10.1097/00005176-198803000-00002. [DOI] [PubMed] [Google Scholar]
  27. Wene J. D., Connor W. E., DenBesten L. The development of essential fatty acid deficiency in healthy men fed fat-free diets intravenously and orally. J Clin Invest. 1975 Jul;56(1):127–134. doi: 10.1172/JCI108061. [DOI] [PMC free article] [PubMed] [Google Scholar]

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