Abstract
Proteome-pI is an online database containing information about predicted isoelectric points for 5029 proteomes calculated using 18 methods. The isoelectric point, the pH at which a particular molecule carries no net electrical charge, is an important parameter for many analytical biochemistry and proteomics techniques, especially for 2D gel electrophoresis (2D-PAGE), capillary isoelectric focusing, liquid chromatography–mass spectrometry and X-ray protein crystallography. The database, available at http://isoelectricpointdb.org allows the retrieval of virtual 2D-PAGE plots and the development of customised fractions of proteome based on isoelectric point and molecular weight. Moreover, Proteome-pI facilitates statistical comparisons of the various prediction methods as well as biological investigation of protein isoelectric point space in all kingdoms of life. For instance, using Proteome-pI data, it is clear that Eukaryotes, which evolved tight control of homeostasis, encode proteins with pI values near the cell pH. In contrast, Archaea living frequently in extreme environments can possess proteins with a wide range of isoelectric points. The database includes various statistics and tools for interactive browsing, searching and sorting. Apart from data for individual proteomes, datasets corresponding to major protein databases such as UniProtKB/TrEMBL and the NCBI non-redundant (nr) database have also been precalculated and made available in CSV format.
INTRODUCTION
Isoelectric point (pI) is the pH at which a particular molecule carries no net electrical charge. For polypeptide chains, pI depends primarily on the dissociation constants (pKa) for the ionisable groups of the seven charged amino acids: glutamate, aspartate, cysteine, tyrosine, histidine, lysine and arginine. Moreover, the charge of the terminal groups (NH2 and COOH) can affect the pI of short peptides. It is also important to consider posttranslational modifications, the exposure of charged residues to solvent, the Born effect (dehydration), hydrogen bonds (charge-dipole interactions), and charge-charge interactions (1). pI has broad usage in currently used biochemical and proteomic techniques. For example, during electrophoresis, the direction of protein migration on the gel depends on the charge. Thus, it is possible to separate proteins in a gel based on their pI. Given the sequence, we can try to computationally predict pI using the Henderson–Hasselbalch equation (2), by calculating the charge of the molecule at a certain pH using pKa values of charged residues. More than 600 different pKa values have so far been reported for the ionisable groups of amino acids (3). The final result, predicted pI, will most likely be different than the real one, given that many proteins are chemically modified (e.g., amino acids can be phosphorylated, methylated, or acetylated), and this can influence their charge. Nevertheless, even an approximate isoelectric point is a highly valuable and frequently used parameter.
In the past, much work has gone into creating databases storing experimentally verified pI values for proteins, yet none of these databases contains more than five thousand proteins (4,5), which is very few compared to the protein sequence data currently available. Thus, Proteome-pI database is an attempt to decrease this gap; hopefully, it will expand the body of knowledge regarding isoelectric points in a more genome-wide fashion.
MATERIALS AND METHODS
Sequences
Protein sequences of model organisms were obtained from UniProt as of April 2016, release 2016_04 (6). This includes 5029 complete proteomes (with splicing isoforms for Eukaryote) from the entire tree of life. In total, protein isoelectric point, molecular weights and other statistics were calculated for >21 million protein sequences (Table 1).
Table 1. General statistics of the Proteome-pI database.
Number of proteomes | Total number of proteins | Mean number of proteins (±SD) | Mean size of proteins (±SD) | Mean mw of proteins (±SD) | |
---|---|---|---|---|---|
Viruses | 504 | 20 920 | 42 ± 89 | 297 ± 375 | 33 ± 42 |
Archaea | 135 | 318 388 | 2358 ± 920 | 283 ± 212 | 31 ± 23 |
Bacteria | 3776 | 12 082 903 | 3200 ± 2510 | 311 ± 240 | 34 ± 26 |
Eukaryote | 614 | 9 299 039 | 15 145 ± 11 830 | 438 ± 429 | 49 ± 48 |
Eukaryote (major) | 614 | 8 629 591 | 14 055 ± 9899 | 434 ± 416 | 48 ± 46 |
Eukaryote (minor) | 448 | 669 448 | 1494 ± 5130 | 495 ± 564 | 55 ± 63 |
mw—molecular weight in kDa; for more statistics, see Supplementary Table S1. ‘Major’ and ‘minor’ refer to splicing isoforms of proteins used for calculation of the statistics.
Predictions
To predict isoelectric points, Proteome-pI currently uses 18 different algorithms and programs, which can be divided into three categories. The first category consists of methods that predict the isoelectric point based on the Henderson–Hasselbalch equation with different pKa values corresponding to different charged groups (2). Those methods usually use nine different pKa values established empirically in separate experiments (seven pKa values for charged amino acids and two for polypeptide chain termini). For example, pKa values obtained by Thurlkill et al. were measured in 0.1 M KCl at 25°C using alanine pentapeptides with a charged residue in the centre and with blocked terminal groups (7). Further, nine-parameter models are used for calculation of isoelectric points in methods named after the lead author of the study or the source of the pKa values: EMBOSS (8), DTASelect (9), Solomons (10), Sillero (11), Rodwell (12), Wikipedia, Lehninger (13), Grimsley (3), Toseland (14), Thurlkill (7), Nozaki (15) and Dawson (16). Additionally some algorithms use different numbers of pKa values (Patrickios (17) uses only six, Bjellqvist (18) uses 17, and ProMoST (19) uses 72 pKa values depending on the location of amino acid with respect to the protein termini). In the next category, we have IPC_protein and IPC_peptide models, which use computationally optimised nine-parameter pKa sets (20). Finally, the consensus from all methods apart from Patrickios (highly simplified model with only six parameters) is also reported.
RESULTS
Database use
The Proteome-pI database incorporates multiple browsing and searching tools. First, it can be searched and browsed by organism name, average isoelectric point, molecular weight or amino acid frequencies (see also Table 2). Proteins with extreme pI values are also available. For individual proteomes, users can retrieve proteins of interest given the method, isoelectric point and molecular weight ranges (this particular feature can be highly useful to limit potential targets in analysis of 2D-PAGE gels or before conducting mass spectrometry). Additionally, precalculated fractions of proteins according to isoelectric point are also available. Finally, some general statistics (total number of proteins, amino acids, average sequence length, amino acid frequency) and links to other databases (UniProt, NCBI) can be found (see Figure 1 for an example).
Table 2. Amino acid frequency for the kingdoms of life in the Proteome-pI database.
Kingdom | Ala | Cys | Asp | Glu | Phe | Gly | His | Ile | Lys | Leu | Met | Asn | Pro | Gln | Arg | Ser | Thr | Val | Trp | Tyr | Total amino acids |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Viruses | 6.61 | 1.76 | 5.81 | 6.04 | 4.25 | 5.79 | 2.15 | 6.53 | 6.35 | 8.84 | 2.46 | 5.41 | 4.62 | 3.39 | 5.24 | 7.06 | 6.06 | 6.50 | 1.19 | 3.94 | 6 150 189 |
Archaea | 8.20 | 0.98 | 6.21 | 7.69 | 3.86 | 7.58 | 1.77 | 7.03 | 5.27 | 9.31 | 2.35 | 3.68 | 4.26 | 2.38 | 5.51 | 6.17 | 5.44 | 7.80 | 1.03 | 3.45 | 89 488 664 |
Bacteria | 10.06 | 0.94 | 5.59 | 6.15 | 3.89 | 7.76 | 2.06 | 5.89 | 4.68 | 10.09 | 2.38 | 3.58 | 4.61 | 3.58 | 5.88 | 5.85 | 5.52 | 7.27 | 1.27 | 2.94 | 3 716 982 916 |
Eukaryota | 7.63 | 1.76 | 5.40 | 6.42 | 3.87 | 6.33 | 2.44 | 5.10 | 5.64 | 9.29 | 2.25 | 4.28 | 5.41 | 4.21 | 5.71 | 8.34 | 5.56 | 6.20 | 1.24 | 2.87 | 3 743 221 293 |
All | 8.76 | 1.38 | 5.49 | 6.32 | 3.87 | 7.03 | 2.26 | 5.49 | 5.19 | 9.68 | 2.32 | 3.93 | 5.02 | 3.90 | 5.78 | 7.14 | 5.53 | 6.73 | 1.25 | 2.91 | 7 555 843 062 |
*Similar statistics for all 5029 proteomes included in Proteome-pI are available online on individual subpages. For di-amino acid frequencies see Supplementary Table S2.
Moreover, apart from the data for individual proteomes, one can also obtain precalculated isoelectric points from all major protein databases, including nr (21), UniProt, PDB (22) and SwissProt (23) (more details in Supplementary Data).
DISCUSSION
The main content of the Proteome-pI database is the comprehensive isoelectric point prediction using numerous methods. The isoelectric point—the pH at which a particular molecule carries no net electrical charge—is an important parameter for many analytical biochemistry and proteomics techniques, such as 2D-PAGE gel electrophoresis (24,25), capillary isoelectric focusing (26), liquid chromatography–mass spectrometry (LC–MS) (27) and X-ray protein crystallography (28,29). Additional goals of the database include facilitating biological investigation of protein isoelectric point space. For instance, it is well known that distribution of protein isoelectric points of proteomes is bimodal, with a low fraction of proteins having pI values close to the cell physiological pH (Supplementary Figure S1) (30). Interestingly, if we divide proteomes into the kingdoms of life, one can notice that Eukaryota have the largest proteins restricted to narrow isoelectric point range. On the other side, Archaea possess usually small proteins, but the isoelectric points of their proteins can vary significantly (Figure 2). This is most likely due to the adaptation to the extreme conditions in which many Archaea live (31). Finally, viruses form a completely separate group. Their proteins have isoelectric point which is strongly correlated with the pI of its host proteins and therefore can vary significantly. Simultaneously, the molecular weight of viral proteins is significantly lower than that of host proteins due to the compactness of virions (significant evolutionary pressure to minimise the overall size) (32).
It should be noted that there is at least one other similar database storing isoelectric points for some proteomes. The JVirGel website (33) containspI data for 227 relatively small, prokaryotic proteomes, precalculated using only one method. In contrast, the Proteome-pI database aggregates predictions of isoelectric points calculated by 18 different methods and algorithms across >5000 proteomes from all kingdoms of life (over 21 million proteins).
Future work
The principal future goal is to include more isoelectric point algorithms and proteomes for further investigation. The next future goal is to provide more tools for online analysis, e.g., tools for Gene Ontology searching (34). Another possible extension could be to add putative digestion products of trypsin and their respective isoelectric points (35). We will be grateful for any contribution to the database from the community.
AVAILABILITY
All data in the Proteome-pI database are available for download free of charge. Proteome-pI can be accessed at http://isoelectricpointdb.org The database will be available at given web address for at least ten years.
Supplementary Material
ACKNOWLEDGEMENTS
Proteome-pI author acknowledges all authors of previous works related to different pKa sets and databases, especially developers of UniProt database.
SUPPLEMENTARY DATA
Supplementary Data are available at NAR Online.
FUNDING
Funding for open access charge: The open access publication charge for this paper has been waived by Oxford University Press—NAR Editorial Board members are entitled to one free paper per year in recognition of their work on behalf of the journal.
Conflict of interest statement. None declared.
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