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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 May 1;89(9):3927–3931. doi: 10.1073/pnas.89.9.3927

A T-cell dormant state in the autoimmune process of nonobese diabetic mice treated with complete Freund's adjuvant.

D Ulaeto 1, P E Lacy 1, D M Kipnis 1, O Kanagawa 1, E R Unanue 1
PMCID: PMC525604  PMID: 1570315

Abstract

Three experimental manipulations showed the suppressive effect of injection of complete Freund's adjuvant (CFA) in the diabetic state of nonobese diabetic (NOD) mice. (i) Diabetes was inhibited in young NOD mice injected with the adjuvant. (ii) Recurrence of diabetes in mice transplanted with pancreatic islets was also inhibited when the recipients were injected with CFA. (iii) Injection of spleen lymphocytes from diabetic mice into male NOD mice transferred the diabetic state, but this transfer was markedly suppressed when the recipients were treated with CFA. In all three cases the spleen cells from the normoglycemic mice treated with CFA induced diabetes when transferred into NOD male mice. CFA, therefore, induces a state of T-cell dormancy, in which the islets are no longer subject to an immune attack.

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Selected References

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  1. Asherson G. L., Allwood G. G. Depression of delayed hypersensitivity by pretreatment with Freund-type adjuvants. I. Description of the phenomenon. Clin Exp Immunol. 1971 Aug;9(2):249–258. [PMC free article] [PubMed] [Google Scholar]
  2. Bendelac A., Carnaud C., Boitard C., Bach J. F. Syngeneic transfer of autoimmune diabetes from diabetic NOD mice to healthy neonates. Requirement for both L3T4+ and Lyt-2+ T cells. J Exp Med. 1987 Oct 1;166(4):823–832. doi: 10.1084/jem.166.4.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harada M., Kishimoto Y., Makino S. Prevention of overt diabetes and insulitis in NOD mice by a single BCG vaccination. Diabetes Res Clin Pract. 1990 Jan;8(2):85–89. doi: 10.1016/0168-8227(90)90017-n. [DOI] [PubMed] [Google Scholar]
  4. Hempel K., Freitag A., Freitag B., Endres B., Mai B., Liebaldt G. Unresponsiveness to experimental allergic encephalomyelitis in Lewis rats pretreated with complete Freund's adjuvant. Int Arch Allergy Appl Immunol. 1985;76(3):193–199. doi: 10.1159/000233691. [DOI] [PubMed] [Google Scholar]
  5. Jacob C. O., Aiso S., Michie S. A., McDevitt H. O., Acha-Orbea H. Prevention of diabetes in nonobese diabetic mice by tumor necrosis factor (TNF): similarities between TNF-alpha and interleukin 1. Proc Natl Acad Sci U S A. 1990 Feb;87(3):968–972. doi: 10.1073/pnas.87.3.968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lacy P. E., Kostianovsky M. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes. 1967 Jan;16(1):35–39. doi: 10.2337/diab.16.1.35. [DOI] [PubMed] [Google Scholar]
  7. Lisak R. P., Kies M. W. Mycobacterial suppression of delayed hypersensitivity in experimental allergic encephalomyelitis. Proc Soc Exp Biol Med. 1968 May;128(1):214–218. doi: 10.3181/00379727-128-32981. [DOI] [PubMed] [Google Scholar]
  8. McInerney M. F., Pek S. B., Thomas D. W. Prevention of insulitis and diabetes onset by treatment with complete Freund's adjuvant in NOD mice. Diabetes. 1991 Jun;40(6):715–725. doi: 10.2337/diab.40.6.715. [DOI] [PubMed] [Google Scholar]
  9. Miller B. J., Appel M. C., O'Neil J. J., Wicker L. S. Both the Lyt-2+ and L3T4+ T cell subsets are required for the transfer of diabetes in nonobese diabetic mice. J Immunol. 1988 Jan 1;140(1):52–58. [PubMed] [Google Scholar]
  10. Oldstone M. B. Prevention of type I diabetes in nonobese diabetic mice by virus infection. Science. 1988 Jan 29;239(4839):500–502. doi: 10.1126/science.3277269. [DOI] [PubMed] [Google Scholar]
  11. Reinisch C. L., Gleiner N. A., Schlossman S. F. Adjuvant regulation of T cell function. J Immunol. 1976 Mar;116(3):710–715. [PubMed] [Google Scholar]
  12. Root-Bernstein R. S., Yurochko F., Westall F. C. Clinical suppression of experimental allergic encephalomyelitis by muramyl dipeptide "adjuvant". Brain Res Bull. 1986 Oct;17(4):473–476. doi: 10.1016/0361-9230(86)90213-3. [DOI] [PubMed] [Google Scholar]
  13. Sadelain M. W., Qin H. Y., Lauzon J., Singh B. Prevention of type I diabetes in NOD mice by adjuvant immunotherapy. Diabetes. 1990 May;39(5):583–589. doi: 10.2337/diab.39.5.583. [DOI] [PubMed] [Google Scholar]
  14. Satoh J., Seino H., Abo T., Tanaka S., Shintani S., Ohta S., Tamura K., Sawai T., Nobunaga T., Oteki T. Recombinant human tumor necrosis factor alpha suppresses autoimmune diabetes in nonobese diabetic mice. J Clin Invest. 1989 Oct;84(4):1345–1348. doi: 10.1172/JCI114304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Satoh J., Shintani S., Oya K., Tanaka S., Nobunaga T., Toyota T., Goto Y. Treatment with streptococcal preparation (OK-432) suppresses anti-islet autoimmunity and prevents diabetes in BB rats. Diabetes. 1988 Sep;37(9):1188–1194. doi: 10.2337/diab.37.9.1188. [DOI] [PubMed] [Google Scholar]
  16. Shintani S., Satoh J., Seino H., Goto Y., Toyota T. Mechanism of action of a streptococcal preparation (OK-432) in prevention of autoimmune diabetes in NOD mice. Suppression of generation of effector cells for pancreatic B cell destruction. J Immunol. 1990 Jan 1;144(1):136–141. [PubMed] [Google Scholar]
  17. Toyota T., Satoh J., Oya K., Shintani S., Okano T. Streptococcal preparation (OK-432) inhibits development of type I diabetes in NOD mice. Diabetes. 1986 Apr;35(4):496–499. doi: 10.2337/diab.35.4.496. [DOI] [PubMed] [Google Scholar]
  18. Weber F., Hempel K. Protection against experimental allergic encephalomyelitis with complete Freund's adjuvant is unaffected by ionizing irradiation. Int Arch Allergy Appl Immunol. 1987;83(2):174–177. doi: 10.1159/000234351. [DOI] [PubMed] [Google Scholar]
  19. Wicker L. S., Miller B. J., Mullen Y. Transfer of autoimmune diabetes mellitus with splenocytes from nonobese diabetic (NOD) mice. Diabetes. 1986 Aug;35(8):855–860. doi: 10.2337/diab.35.8.855. [DOI] [PubMed] [Google Scholar]
  20. Zola H. Modulation of the immune response to transplantation antigens. I. The effects of immunization using adjuvants on the immune response of mice to a tumour allograft. Clin Exp Immunol. 1972 Aug;11(4):585–593. [PMC free article] [PubMed] [Google Scholar]

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