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. 1978 Dec;28(3):851–864. doi: 10.1128/jvi.28.3.851-864.1978

Physical mapping of BK virus DNA with SacI, MboII, and AluI restriction endonucleases.

R C Yang, R Wu
PMCID: PMC525810  PMID: 215783

Abstract

A new restriction endonuclease, SacI from Streptomyces achromogenes cleaves BK virus (strain MM) DNA into 3 fragments, whereas MboII from Moraxella bovis and AluI from Arthrobacter luteus give 22 and 30 fragments, respectively. All these specific DNA fragments were ordered and mapped on the viral genome by two methods first, by the reciprocal digestion method using uniformly 32P-labeled DNA; and second, by the partial digestion technique using the single-end 32P-labeled DNA. This study, together with those reported earlier, defined the location of 90 cleavage sites on the BK virus DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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