Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1979 Dec;32(3):796–802. doi: 10.1128/jvi.32.3.796-802.1979

Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line.

P L Marion, F H Salazar, J J Alexander, W S Robinson
PMCID: PMC525927  PMID: 92575

Abstract

The PLC/PRF/5 cell line derived from a human hepatoma produces hepatitis B surface antigen (HBsAg) in 22-nm particles of the same buoyant density as those found in the serum of infected patients. The HBsAg particles from this cell line were labeled with [35S]methionine and purified, and the polypeptides were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with those of serum-derived particles. The two major polypeptides of serum-derived HBsAg particles (p20 and p23) were found in the same relative amounts in the particles from the cell line. The three smallest of the five minor components observed in HBsAg particles from serum were present in particles from the cell line. These polypeptides (p31, p36, and p43), as well as p20 and p23, were precipitated with anti-HBs-containing serum. The two largest polypeptides of serum particles (p49 and p66) were not detected in particles from these cells. When the PLC/PRF/5 HBsAg particles were radiolabeled with tritiated sugars, p23, and not p20, was found to contain radioactivity, indicating that the pattern of polypeptide glycosylation is similar to that of serum HBsAg. None of the other possible gene products of hepatitis B virus was detected in the PLC/PRF/5-derived HBsAg particles, in the cells, or in the cell supernatants.

Full text

PDF
796

Images in this article

799Image
on p.799
800Image
on p.800
800Image
on p.800
801Image
on p.801

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barker L. F., Chisari F. V., McGrath P. P., Dalgard D. W., Kirschstein R. L., Almeida J. D., Edington T. S., Sharp D. G., Peterson M. R. Transmission of type B viral hepatitis to chimpanzees. J Infect Dis. 1973 Jun;127(6):648–662. doi: 10.1093/infdis/127.6.648. [DOI] [PubMed] [Google Scholar]
  2. Blumberg B. S., Larouzé B., London W. T., Werner B., Hesser J. E., Millman I., Saimot G., Payet M. The relation of infection with the hepatitis B agent to primary hepatic carcinoma. Am J Pathol. 1975 Dec;81(3):669–682. [PMC free article] [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. Dreesman G. R., Chairez R., Suarez M., Hollinger F. B., Courtney R. J., Melnick J. L. Production of antibody to individual polypeptides derived from purified hepatitis B surface antigen. J Virol. 1975 Sep;16(3):508–515. doi: 10.1128/jvi.16.3.508-515.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gold J. W., Shih J. W., Purcell R. H., Gerin J. L. Characterization of antibodies to the structural polypeptides of HGSAg: evidence for subtype-specific determinants. J Immunol. 1976 Oct;117(4):1404–1406. [PubMed] [Google Scholar]
  6. Gudat F., Bianchi L., Sonnabend W., Thiel G., Aenishaenslin W., Stalder G. A. Pattern of core and surface expression in liver tissue reflects state of specific immune response in hepatitis B. Lab Invest. 1975 Jan;32(1):1–9. [PubMed] [Google Scholar]
  7. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  8. Kew M. C., Geddes E. W., Macnab G. M., Bersohn I. Hepatitis-B antigen and cirrhosis in Bantu patients with primary liver cancer. Cancer. 1974 Sep;34(3):538–541. doi: 10.1002/1097-0142(197409)34:3<539::aid-cncr2820340310>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. MacNab G. M., Alexander J. J., Lecatsas G., Bey E. M., Urbanowicz J. M. Hepatitis B surface antigen produced by a human hepatoma cell line. Br J Cancer. 1976 Nov;34(5):509–515. doi: 10.1038/bjc.1976.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackay P., Burrell C. J. Examination of the polypeptides of hepatitis B surface antigen. J Gen Virol. 1976 Nov;33(2):181–191. doi: 10.1099/0022-1317-33-2-181. [DOI] [PubMed] [Google Scholar]
  13. Magnius L. O., Lindholm A., Lundin P., Iwarson S. A new antigen-antibody system. Clinical significance in long-term carriers of hepatitis B surface antigen. JAMA. 1975 Jan 27;231(4):356–359. doi: 10.1001/jama.231.4.356. [DOI] [PubMed] [Google Scholar]
  14. Mathiesen L. R., Feinstone S. M., Wong D. C., Skinhoej P., Purcell R. H. Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay. J Clin Microbiol. 1978 Feb;7(2):184–193. doi: 10.1128/jcm.7.2.184-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peterson D. L., Roberts I. M., Vyas G. N. Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1530–1534. doi: 10.1073/pnas.74.4.1530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Purcell R. H., Gerin J. L., Almeida J. B., Holland P. V. Radioimmunoassay for the detection of the core of the Dane particle and antibody to it. Intervirology. 1974;2(4):231–243. doi: 10.1159/000149428. [DOI] [PubMed] [Google Scholar]
  17. Rao K. R., Vyas G. N. Hepatitis B antigen activity in protein subunits produced by sonicaation. Nat New Biol. 1973 Feb 21;241(112):240–241. doi: 10.1038/newbio241240a0. [DOI] [PubMed] [Google Scholar]
  18. Robinson W. S. The genome of hepatitis B virus. Annu Rev Microbiol. 1977;31:357–377. doi: 10.1146/annurev.mi.31.100177.002041. [DOI] [PubMed] [Google Scholar]
  19. Shih J. W., Gerin J. L. Immunochemistry of hepatitis B surface antigen (HBsAg): preparation and characterization of antibodies to the constituent polypeptides. J Immunol. 1975 Sep;115(3):634–639. [PubMed] [Google Scholar]
  20. Shih J. W., Gerin J. L. Proteins of hepatitis B surface antigen: amino acid compositions of the major polypeptides. J Virol. 1977 Mar;21(3):1219–1222. doi: 10.1128/jvi.21.3.1219-1222.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Szmuness W. Hepatocellular carcinoma and the hepatitis B virus: evidence for a causal association. Prog Med Virol. 1978;24:40–69. [PubMed] [Google Scholar]
  22. Takahashi K., Akahane Y., Gotanda T., Mishiro T., Imai M., Miyakawa Y., Mayumi M. Demonstration of hepatitis B e antigen in the core of Dane particles. J Immunol. 1979 Jan;122(1):275–279. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES