Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1978 Mar;25(3):824–830. doi: 10.1128/jvi.25.3.824-830.1978

Characterization of the 5'-terminal capped structures of late simian virus 40-specific mRNA.

G Haegeman, W Fiers
PMCID: PMC525976  PMID: 205672

Abstract

32P-labeled, late simian virus 40-specific RNA was isoalted from infected CV1 cells and completely degraded with RNase T2 and bacterial alkaline phosphatase. The RNase-resistant material was fractionated two dimensionally and further characterized with Penicillium nuclease and nucleotide pyrophosphatase. Two major 5' termini were identified in late simian virus 40 RNA, namely, 7-methyl Gppp 2',6-dimethyl ApUp and 7-methyl Gppp 2',6-dimethyl Ap 2'-methyl, UpUp. Both 5' termini are present in unfractionated viral RNA as well as in the separated 16S and 19S species. As both caps differ only in secondary modification, it is possible that they are derived from the same site on the DNA. The relatively higher cap II content of the 16S mRNA may be related to its slower rate of turnover.

Full text

PDF
829

Images in this article

826Image
on p.826
827Image
on p.827
828Image
on p.828
829Image
on p.829

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Cory S. Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA. Nature. 1975 May 1;255(5503):28–33. doi: 10.1038/255028a0. [DOI] [PubMed] [Google Scholar]
  2. Aloni Y., Shani M., Reuveni Y. RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2587–2591. doi: 10.1073/pnas.72.7.2587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  4. Berget S. M., Moore C., Sharp P. A. Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171–3175. doi: 10.1073/pnas.74.8.3171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cory S., Adams J. M. The modified 5'-terminal sequences in messenger RNA of mouse myeloma cells. J Mol Biol. 1975 Dec 25;99(4):519–547. doi: 10.1016/s0022-2836(75)80170-7. [DOI] [PubMed] [Google Scholar]
  6. Desrosiers R. C., Friderici K. H., Rottman F. M. Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5' terminus. Biochemistry. 1975 Oct 7;14(20):4367–4374. doi: 10.1021/bi00691a004. [DOI] [PubMed] [Google Scholar]
  7. Fareed G. C., Davoli D. Molecular biology of papovaviruses. Annu Rev Biochem. 1977;46:471–522. doi: 10.1146/annurev.bi.46.070177.002351. [DOI] [PubMed] [Google Scholar]
  8. Furuichi Y., Morgan M., Shatkin A. J., Jelinek W., Salditt-Georgieff M., Darnell J. E. Methylated, blocked 5 termini in HeLa cell mRNA. Proc Natl Acad Sci U S A. 1975 May;72(5):1904–1908. doi: 10.1073/pnas.72.5.1904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gelinas R. E., Roberts R. J. One predominant 5'-undecanucleotide in adenovirus 2 late messenger RNAs. Cell. 1977 Jul;11(3):533–544. doi: 10.1016/0092-8674(77)90071-x. [DOI] [PubMed] [Google Scholar]
  10. Gilboa E., Prives C. L., Aviv H. Purification of SV-40 messenger RNA by hybridization to SV-40 DNA covalently bound to Sepharose. Biochemistry. 1975 Sep 23;14(19):4215–4220. doi: 10.1021/bi00690a010. [DOI] [PubMed] [Google Scholar]
  11. Khoury G., Carter B. J., Ferdinand F. J., Howley P. M., Brown M., Martin M. A. Genome localization of simian virus 40 RNA species. J Virol. 1976 Mar;17(3):832–840. doi: 10.1128/jvi.17.3.832-840.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klessig D. F. Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions. Cell. 1977 Sep;12(1):9–21. doi: 10.1016/0092-8674(77)90181-7. [DOI] [PubMed] [Google Scholar]
  13. Krug R. M., Morgan M. A., Shatkin A. J. Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures. J Virol. 1976 Oct;20(1):45–53. doi: 10.1128/jvi.20.1.45-53.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  15. Lavi S., Shatkin A. J. Methylated simian virus 40-specific RNA from nuclei and cytoplasm of infected BSC-1 cells. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2012–2016. doi: 10.1073/pnas.72.6.2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marx J. L. Viral messenger structure: some surprising new developments. Science. 1977 Aug 26;197(4306):853–923. doi: 10.1126/science.197.4306.853. [DOI] [PubMed] [Google Scholar]
  17. Moss B., Koczot F. Sequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA. J Virol. 1976 Feb;17(2):385–392. doi: 10.1128/jvi.17.2.385-392.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moyer S. A., Banerjee A. K. In vivo methylation of vesicular stomatitis virus and its host-cell messenger RNA species. Virology. 1976 Apr;70(2):339–351. doi: 10.1016/0042-6822(76)90276-2. [DOI] [PubMed] [Google Scholar]
  19. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perry R. P., Kelley D. E., Friderici K., Rottman F. The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus. Cell. 1975 Apr;4(4):387–394. doi: 10.1016/0092-8674(75)90159-2. [DOI] [PubMed] [Google Scholar]
  21. Perry R. P., Kelley D. E. Kinetics of formation of 5' terminal caps in mRNA. Cell. 1976 Jul;8(3):433–442. doi: 10.1016/0092-8674(76)90156-2. [DOI] [PubMed] [Google Scholar]
  22. Rose J. K. Heterogneeous 5'-terminal structures occur on vesicular stomatitis virus mRNAs. J Biol Chem. 1975 Oct 25;250(20):8098–8104. [PubMed] [Google Scholar]
  23. Rottman F., Shatkin A. J., Perry R. P. Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing. Cell. 1974 Nov;3(3):197–199. doi: 10.1016/0092-8674(74)90131-7. [DOI] [PubMed] [Google Scholar]
  24. Sambrook J. Adenovirus amazes at Cold Spring Harbor. Nature. 1977 Jul 14;268(5616):101–104. doi: 10.1038/268101a0. [DOI] [PubMed] [Google Scholar]
  25. Shatkin A. J. Capping of eucaryotic mRNAs. Cell. 1976 Dec;9(4 Pt 2):645–653. doi: 10.1016/0092-8674(76)90128-8. [DOI] [PubMed] [Google Scholar]
  26. Sommer S., Salditt-Georgieff M., Bachenheimer S., Darnell J. E., Furuichi Y., Morgan M., Shatkin A. J. The methylation of adenovirus-specific nuclear and cytoplasmic RNA. Nucleic Acids Res. 1976 Mar;3(3):749–765. doi: 10.1093/nar/3.3.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Southern E. M. An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange cellulose. Anal Biochem. 1974 Nov;62(1):317–318. doi: 10.1016/0003-2697(74)90395-9. [DOI] [PubMed] [Google Scholar]
  28. Volckaert G., Fiers W. Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: double digestion and pancreatic ribonuclease analyses. Anal Biochem. 1977 Nov;83(1):228–239. doi: 10.1016/0003-2697(77)90531-0. [DOI] [PubMed] [Google Scholar]
  29. Volckaert G., Jou W. M., Fiers W. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem. 1976 May 7;72:433–446. doi: 10.1016/0003-2697(76)90551-0. [DOI] [PubMed] [Google Scholar]
  30. Wei C. M., Gershowitz A., Moss B. Methylated nucleotides block 5' terminus of HeLa cell messenger RNA. Cell. 1975 Apr;4(4):379–386. doi: 10.1016/0092-8674(75)90158-0. [DOI] [PubMed] [Google Scholar]
  31. Weinberg R. A., Ben-Ishai Z., Newbold J. E. Simian virus 40 transcription in productively infected and transformed cells. J Virol. 1974 Jun;13(6):1263–1273. doi: 10.1128/jvi.13.6.1263-1273.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Weinberg R. A., Warnaar S. O., Winocour E. Isolation and characterization of simian virus 40 ribonucleic acid. J Virol. 1972 Aug;10(2):193–201. doi: 10.1128/jvi.10.2.193-201.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yang N. S., Manning R. F., Gage L. P. The blocked and methylated 5' terminal sequence of a specific cellular messenger: the mRNA for silk fibroin of Bombyx mori. Cell. 1976 Mar;7(3):339–347. doi: 10.1016/0092-8674(76)90163-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES