Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Oct;172(10):5697–5705. doi: 10.1128/jb.172.10.5697-5705.1990

Characterization of the minimum replicon of the broad-host-range plasmid pTF-FC2 and similarity between pTF-FC2 and the IncQ plasmids.

R A Dorrington 1, D E Rawlings 1
PMCID: PMC526885  PMID: 2120189

Abstract

The nucleotide sequence of a 3,202-base-pair fragment which contained the minimum region required for replication of the broad-host-range plasmid, pTF-FC2, has been determined. At least five open reading frames and a region that affected the host range were identified. Proteins corresponding in size and location to four of the five open reading frames were produced in an in vitro transcription-translation system. The predicted amino acid sequences of two of the proteins were aligned with those of the RepA and RepC proteins of the broad-host-range IncQ plasmid RSF1010 and found to be 43 and 60% homologous, respectively. Despite this similarity, neither the RepA nor the RepC protein of the IncQ plasmid was able to complement mutations in the pTF-FC2 repA and repC genes. Although there was a considerable amount of DNA homology between pTF-FC2 and RSF1010 in the oriV region and the region coding for the RepA and RepC proteins, no other homology between the two plasmids at either the DNA or protein level could be detected.

Full text

PDF
5705

Images in this article

5702Image
on p.5702

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagdasarian M., Timmis K. N. Host: vector systems for gene cloning in Pseudomonas. Curr Top Microbiol Immunol. 1982;96:47–67. doi: 10.1007/978-3-642-68315-2_4. [DOI] [PubMed] [Google Scholar]
  2. Barth P. T., Grinter N. J. Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides. J Bacteriol. 1974 Nov;120(2):618–630. doi: 10.1128/jb.120.2.618-630.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  4. Chung C. T., Miller R. H. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res. 1988 Apr 25;16(8):3580–3580. doi: 10.1093/nar/16.8.3580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dorrington R. A., Rawlings D. E. Identification and sequence of the basic replication region of a broad-host-range plasmid isolated from Thiobacillus ferrooxidans. J Bacteriol. 1989 May;171(5):2735–2739. doi: 10.1128/jb.171.5.2735-2739.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guerry P., van Embden J., Falkow S. Molecular nature of two nonconjugative plasmids carrying drug resistance genes. J Bacteriol. 1974 Feb;117(2):619–630. doi: 10.1128/jb.117.2.619-630.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haring V., Scholz P., Scherzinger E., Frey J., Derbyshire K., Hatfull G., Willetts N. S., Bagdasarian M. Protein RepC is involved in copy number control of the broad host range plasmid RSF1010. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6090–6094. doi: 10.1073/pnas.82.18.6090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harrison A. P., Jr The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. Annu Rev Microbiol. 1984;38:265–292. doi: 10.1146/annurev.mi.38.100184.001405. [DOI] [PubMed] [Google Scholar]
  10. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  11. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kim K., Meyer R. J. Copy-number of broad host-range plasmid R1162 is regulated by a small RNA. Nucleic Acids Res. 1986 Oct 24;14(20):8027–8046. doi: 10.1093/nar/14.20.8027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lin L. S., Kim Y. J., Meyer R. J. The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro. Mol Gen Genet. 1987 Jul;208(3):390–397. doi: 10.1007/BF00328129. [DOI] [PubMed] [Google Scholar]
  14. Meyer R., Hinds M., Brasch M. Properties of R1162, a broad-host-range, high-copy-number plasmid. J Bacteriol. 1982 May;150(2):552–562. doi: 10.1128/jb.150.2.552-562.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Persson C., Nordström K. Control of replication of the broad host range plasmid RSF1010: the incompatibility determinant consists of directly repeated DNA sequences. Mol Gen Genet. 1986 Apr;203(1):189–192. doi: 10.1007/BF00330402. [DOI] [PubMed] [Google Scholar]
  16. Pretorius I. M., Rawlings D. E., O'Neill E. G., Jones W. A., Kirby R., Woods D. R. Nucleotide sequence of the gene encoding the nitrogenase iron protein of Thiobacillus ferrooxidans. J Bacteriol. 1987 Jan;169(1):367–370. doi: 10.1128/jb.169.1.367-370.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ramesar R. S., Abratt V., Woods D. R., Rawlings D. E. Nucleotide sequence and expression of a cloned Thiobacillus ferrooxidans recA gene in Escherichia coli. Gene. 1989 May 15;78(1):1–8. doi: 10.1016/0378-1119(89)90308-9. [DOI] [PubMed] [Google Scholar]
  18. Rawlings D. E., Jones W. A., O'Neill E. G., Woods D. R. Nucleotide sequence of the glutamine synthetase gene and its controlling region from the acidophilic autotroph Thiobacillus ferrooxidans. Gene. 1987;53(2-3):211–217. doi: 10.1016/0378-1119(87)90009-6. [DOI] [PubMed] [Google Scholar]
  19. Rawlings D. E., Pretorius I., Woods D. R. Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli. J Bacteriol. 1984 May;158(2):737–738. doi: 10.1128/jb.158.2.737-738.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rawlings D. E. Sequence and structural analysis of the alpha- and beta-dinitrogenase subunits of Thiobacillus ferrooxidans. Gene. 1988 Sep 30;69(2):337–343. doi: 10.1016/0378-1119(88)90444-1. [DOI] [PubMed] [Google Scholar]
  21. Rawlings D. E., Woods D. R. Mobilization of Thiobacillus ferrooxidans plasmids among Escherichia coli strains. Appl Environ Microbiol. 1985 May;49(5):1323–1325. doi: 10.1128/aem.49.5.1323-1325.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scherzinger E., Bagdasarian M. M., Scholz P., Lurz R., Rückert B., Bagdasarian M. Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins. Proc Natl Acad Sci U S A. 1984 Feb;81(3):654–658. doi: 10.1073/pnas.81.3.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scholz P., Haring V., Wittmann-Liebold B., Ashman K., Bagdasarian M., Scherzinger E. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene. 1989 Feb 20;75(2):271–288. doi: 10.1016/0378-1119(89)90273-4. [DOI] [PubMed] [Google Scholar]
  24. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  25. Zabeau M., Stanley K. K. Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of bacteriophage lambda. EMBO J. 1982;1(10):1217–1224. doi: 10.1002/j.1460-2075.1982.tb00016.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES