Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Oct;80(19):6010–6013. doi: 10.1073/pnas.80.19.6010

Human lymphocyte production of immunoreactive thyrotropin.

E M Smith, M Phan, T E Kruger, D H Coppenhaver, J E Blalock
PMCID: PMC534349  PMID: 6351072

Abstract

Interferon-alpha inducers were previously shown to cause human lymphocyte production of a corticotropin (ACTH)-like peptide. Thyrotropin (TSH) was not produced under these conditions. In contrast, this report shows that a T-cell mitogen (staphylococcal enterotoxin A), which does not induce the ACTH-like peptide, caused human lymphocyte production of an immunoreactive (ir) TSH. Lymphocyte synthesis of the ir TSH was first detectable at 24 hr, peaked at 48 hr, and thereafter declined. NaDodSO4/polyacrylamide gel electrophoresis of intrinsically radiolabeled lymphocyte-derived ir TSH showed radiolabeled peaks at 80, 50, and 26 kilodaltons. These peaks presumably correspond to trimeric, dimeric, and monomeric TSH-like proteins, respectively. Acid treatment and reduction caused the ir TSH to migrate as a 14-kilodalton peak with a 12-kilodalton shoulder in a gel filtration column run in 6 M guanidine . HCl. Thus, the ir TSH seemed to be composed of subunits with molecular masses corresponding to those of the beta and alpha chains of human TSH, respectively. The ir TSH appeared to be glycoprotein because it bound to a concanavalin A affinity column. Taken together these data suggest that in addition to ACTH, human lymphocytes can also produce a TSH-like substance.

Full text

PDF
6013

Images in this article

6011Image
on p.6011

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki N., Wakisaka G., Nagata I. Effects of thyroxine on T-cell counts and tumour cell rejection in mice. Acta Endocrinol (Copenh) 1976 Jan;81(1):104–109. doi: 10.1530/acta.0.0810104. [DOI] [PubMed] [Google Scholar]
  2. Ballou B., McKean D. J., Freedlender E. F., Smithies O. HLA membrane antigens: sequencing by intrinsic radioactivity. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4487–4491. doi: 10.1073/pnas.73.12.4487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blalock J. E., Smith E. M. Human leukocyte interferon: structural and biological relatedness to adrenocorticotropic hormone and endorphins. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5972–5974. doi: 10.1073/pnas.77.10.5972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  5. Cornell J. S., Pierce J. G. The subunits of human pituitary thyroid-stimulating hormone. Isolation, properties, and composition. J Biol Chem. 1973 Jun 25;248(12):4327–4333. [PubMed] [Google Scholar]
  6. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  7. Ingham K. C., Aloj S. M., Edelhoch H. Rates of dissociation and recombination of the subunits of bovine thyrotropin. Arch Biochem Biophys. 1974 Aug;163(2):589–599. doi: 10.1016/0003-9861(74)90518-9. [DOI] [PubMed] [Google Scholar]
  8. Johnson H. M., Smith E. M., Torres B. A., Blalock J. E. Regulation of the in vitro antibody response by neuroendocrine hormones. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4171–4174. doi: 10.1073/pnas.79.13.4171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KURLAND G. S., KROTKOV M. V., FREEDBERG A. S. Oxygen consumption and-thyroxine deiodination by human leukocytes. J Clin Endocrinol Metab. 1960 Jan;20:35–46. doi: 10.1210/jcem-20-1-35. [DOI] [PubMed] [Google Scholar]
  10. Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kumar M. S., Chiang T., Deodhar S. D. Enhancing effect of thyroxine on tumor growth and metastases in syngeneic mouse tumor systems. Cancer Res. 1979 Sep;39(9):3515–3518. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Langford M. P., Stanton G. J., Johnson H. M. Biological effects of staphylococcal enterotoxin A on human peripheral lymphocytes. Infect Immun. 1978 Oct;22(1):62–68. doi: 10.1128/iai.22.1.62-68.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pierce J. G., Liao T., Howard S. M., Shome B., Cornell J. S. Studies on the structure of thyrotropin: its relationship to luteinizing hormone. Recent Prog Horm Res. 1971;27:165–212. doi: 10.1016/b978-0-12-571127-2.50029-8. [DOI] [PubMed] [Google Scholar]
  15. Poretz R. D., Goldstein I. J. An examination of the topography of the saccharide binding sites of concanavalin A and of the forces involved in complexation. Biochemistry. 1970 Jul 7;9(14):2890–2896. doi: 10.1021/bi00816a021. [DOI] [PubMed] [Google Scholar]
  16. SIEGEL E., SACHS B. A. IN VITRO LEUKOCYTE UPTAKE OF 131-I LABELED IODIDE, THYROXINE AND TRIIODOTHYRONINE, AND ITS RELATION TO THYROID FUNCTION. J Clin Endocrinol Metab. 1964 Apr;24:313–318. doi: 10.1210/jcem-24-4-313. [DOI] [PubMed] [Google Scholar]
  17. Sharma S. D., Tsai V., Proffitt M. R. Enhancement of mouse natural killer cell activity by thyroxine. Cell Immunol. 1982 Oct;73(1):83–97. doi: 10.1016/0008-8749(82)90437-3. [DOI] [PubMed] [Google Scholar]
  18. Smith E. M., Blalock J. E. Human lymphocyte production of corticotropin and endorphin-like substances: association with leukocyte interferon. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7530–7534. doi: 10.1073/pnas.78.12.7530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smith E. M., Blalock J. E. The hormonal nature of the interferon system. Tex Rep Biol Med. 1981;41:350–358. [PubMed] [Google Scholar]
  20. Smith E. M., Johnson H. M., Blalock J. E. Staphylococcus aureus protein A induces the production of interferon-alpha in human lymphocytes and interferon-alpha/beta in mouse spleen cells. J Immunol. 1983 Feb;130(2):773–776. [PubMed] [Google Scholar]
  21. Smith E. M., Meyer W. J., Blalock J. E. Virus-induced corticosterone in hypophysectomized mice: a possible lymphoid adrenal axis. Science. 1982 Dec 24;218(4579):1311–1312. doi: 10.1126/science.6183748. [DOI] [PubMed] [Google Scholar]
  22. Takai N. A., Filetti S., Rapoport B. Studies on the bioactivity of radioiodinated highly purified bovine thyrotropin: analytical polyacrylamide gel electrophoresis. Endocrinology. 1981 Oct;109(4):1144–1149. doi: 10.1210/endo-109-4-1144. [DOI] [PubMed] [Google Scholar]
  23. Weintraub B. D., Stannard B. S., Linnekin D., Marshall M. Relationship of glycosylation to de novo thyroid-stimulating hormone biosynthesis and secretion by mouse pituitary tumor cells. J Biol Chem. 1980 Jun 25;255(12):5715–5723. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES