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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 May;87(9):3274–3278. doi: 10.1073/pnas.87.9.3274

Method to identify genomic targets of DNA binding proteins.

L Sompayrac 1, K J Danna 1
PMCID: PMC53882  PMID: 2159142

Abstract

We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.

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Selected References

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