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. 1990 May;87(10):3879–3883. doi: 10.1073/pnas.87.10.3879

Corn metabolites affect growth and virulence of Agrobacterium tumefaciens.

S V Sahi 1, M D Chilton 1, W S Chilton 1
PMCID: PMC54007  PMID: 11607078

Abstract

Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to block growth of A. tumefaciens completely for 220 hr. DIMBOA at 0.1 mM concentration completely inhibited vir gene induction by 100 microM AS and reduced growth rate by 50%. Thus, DIMBOA can be expected to have a significant effect on attempts to transform corn by using A. tumefaciens as a vector.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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