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. 1975 Jul;56(1):109–112. doi: 10.1104/pp.56.1.109

Effect of Vacuum Infiltration on Photosynthetic Gas Exchange in Leaf Tissue

Ian R MacDonald 1
PMCID: PMC541308  PMID: 16659238

Abstract

Using a manometric method, photosynthetic oxygen evolution and 14CO2 fixation have been determined for leaf tissue of Triticum aestivum L., Hordeum vulgare L., Phaseolus vulgaris L., and Lemna minor L. Approximately similar values in the range 0.2 to 0.4 millimoles grams fresh weight−1 hour−1 were obtained for both gases. In tissue subjected to vacuum infiltration, O2 evolution and 14CO2 fixation were barely measurable. It is considered that the elimination of photosynthetic gas exchange results from a decreased supply of CO2 to the chloroplasts. Chopping wheat laminae also leads to a reduction in photosynthetic gas exchange, slices 1 millimeter or less giving only 10 to 20% of the value for whole tissue. Respiration is unaffected by either treatment. Carbonic anhydrase did not improve photosynthetic gas exchange in infiltrated tissue. The use of sliced or vacuum-infiltrated leaf tissue in photosynthetic studies is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler W. L., De Greef J., Roth T. F., Oelze-Karow H. The Influence of Carbonylcyanide-m-chlorophenylhydrazone and 3-(3,4-Dichlorophenyl)-1,1-dimethylurea on the Fusion of Primary Thylakoids and the Formation of Crystalline Fibrils in Bean Leaves Partially Greened in Far Red Light. Plant Physiol. 1972 Jan;49(1):102–104. doi: 10.1104/pp.49.1.102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hernández-Gil R., Schaedle M. Photophosphorylation and Carbon Dioxide Fixation by Chloroplasts Isolated from Populus deltoides. Plant Physiol. 1972 Sep;50(3):375–377. doi: 10.1104/pp.50.3.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jones H. G., Slatyer R. O. Estimation of the transport and carboxylation components of the intracellular limitation to leaf photosynthesis. Plant Physiol. 1972 Aug;50(2):283–288. doi: 10.1104/pp.50.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KREBS H. A. The use of 'CO2 buffers' in manometric measurements of cell metabolism. Biochem J. 1951 Mar;48(3):349–359. doi: 10.1042/bj0480349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Macdonald I. R., Macklon A. E. Anion absorption by etiolated wheat leaves after vacuum infiltration. Plant Physiol. 1972 Mar;49(3):303–306. doi: 10.1104/pp.49.3.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Macdonald I. R., Macklon A. E. Light-enhanced Chloride Uptake by Wheat Laminae: A Comparison of Chopped and Vacuum-infiltrated Tissue. Plant Physiol. 1975 Jul;56(1):105–108. doi: 10.1104/pp.56.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Macnicol P. K., Young R. E., Biale J. B. Metabolic regulation in the senescing tobacco leaf: I. Changes in pattern of p incorporation into leaf disc metabolites. Plant Physiol. 1973 Apr;51(4):793–797. doi: 10.1104/pp.51.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nadler K. D., Herron H. A., Granick S. Development of chlorophyll and hill activity. Plant Physiol. 1972 Mar;49(3):388–392. doi: 10.1104/pp.49.3.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oelze-Karow H., Butler W. L. The development of photophosphorylation and photosynthesis in greening bean leaves. Plant Physiol. 1971 Nov;48(5):621–625. doi: 10.1104/pp.48.5.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Robertson D., Laetsch W. M. Structure and function of developing barley plastids. Plant Physiol. 1974 Aug;54(2):148–159. doi: 10.1104/pp.54.2.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stewart C. R. Effects of proline and carbohydrates on the metabolism of exogenous proline by excised bean leaves in the dark. Plant Physiol. 1972 Nov;50(5):551–555. doi: 10.1104/pp.50.5.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. VOSE P. B., SHIM S. C. EFFECT OF LIGHT AND RELATED FACTORS ON ION ABSORPTION BY BANANA LEAF DISKS. Nature. 1964 Mar 7;201:1047–1048. doi: 10.1038/2011047a0. [DOI] [PubMed] [Google Scholar]

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