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. 1974 Jan;53(1):104–109. doi: 10.1104/pp.53.1.104

The Oxidation of Malate and Exogenous Reduced Nicotinamide Adenine Dinucleotide by Isolated Plant Mitochondria 1

D A Day a, J T Wiskich a
PMCID: PMC541342  PMID: 16658636

Abstract

Exogenous NADH oxidation by cauliflower (Brassica oleracea L.) bud mitochondria was sensitive to antimycin A and gave ADP/O ratios of 1.4 to 1.9. In intact mitochondria, NADH-cytochrome c reductase activity was only slightly inhibited by antimycin A. The antimycin-insensitive activity was associated with the outer membrane. Malate oxidation was sensitive to both rotenone and antimycin A and gave ADP/O values of 2.4 to 2.9. However in the presence of added NAD+, malate oxidation displayed similar properties to exogenous NADH oxidation. In both the presence and absence of added NAD+, malate oxidation was dependent on inorganic phosphate and inhibited by 2-n-butyl malonate.

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Selected References

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