Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1974 May;53(5):742–746. doi: 10.1104/pp.53.5.742

Proteins of Soybean Seeds

I. Isolation and Characterization of the Major Components 1

J E Hill a,2, R W Breidenbach a
PMCID: PMC541437  PMID: 16658781

Abstract

Soybean (Glycine max) storage proteins were characterized by sedimentation and by polyacrylamide gel electrophoresis under dissociating (8 m urea) and nondissociating conditions. Three sedimenting classes of proteins were found, with sedimentation coefficients of 2.2S, 7.5S, and 11.8S. The coefficients were related to the bands obtained by electrophoretic separation. The results support the idea that relatively few proteins make up the bulk of the seed protein.

Full text

PDF
742

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Catsimpoolas N., Ekenstam C., Rogers D. A., Meyer E. W. Protein subunits in dormant and germinating soybean seeds. Biochim Biophys Acta. 1968 Sep 10;168(1):122–131. doi: 10.1016/0005-2795(68)90241-9. [DOI] [PubMed] [Google Scholar]
  2. Catsimpoolas N. Isolation of glycinin subunits by isoelectric focusing in urea-mercaptoethanol. FEBS Lett. 1969 Aug;4(4):259–261. doi: 10.1016/0014-5793(69)80249-8. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. Eldridge A. C., Anderson R. L., Wolf W. J. Polyacrylamide-gel electrophoresis of soybean whey proteins and trypsin inhibitors. Arch Biochem Biophys. 1966 Sep 9;115(3):495–504. doi: 10.1016/0003-9861(66)90068-3. [DOI] [PubMed] [Google Scholar]
  5. Ericson M. C., Chrispeels M. J. Isolation and Characterization of Glucosamine-containing Storage Glycoproteins from the Cotyledons of Phaseolus aureus. Plant Physiol. 1973 Aug;52(2):98–104. doi: 10.1104/pp.52.2.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  8. NAISMITH W. E. Ultracentrifuge studies on soya bean protein. Biochim Biophys Acta. 1955 Feb;16(2):203–210. doi: 10.1016/0006-3002(55)90205-5. [DOI] [PubMed] [Google Scholar]
  9. WOLF W. J., BRIGGS D. R. Ultracentrifugal investigation of the effect of neutral salts on the extraction of soybean proteins. Arch Biochem Biophys. 1956 Jul;63(1):40–49. doi: 10.1016/0003-9861(56)90007-8. [DOI] [PubMed] [Google Scholar]
  10. WOLF W. J., SLY D. A. CHROMATOGRAPHY OF SOYBEAN PROTEINS ON HYDROXYLAPATITE. Arch Biochem Biophys. 1965 Apr;110:47–56. doi: 10.1016/0003-9861(65)90153-0. [DOI] [PubMed] [Google Scholar]
  11. Wu G. J., Bruening G. Two proteins from cowpea mosaic virus. Virology. 1971 Dec;46(3):596–612. doi: 10.1016/0042-6822(71)90063-8. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES