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. 1975 Jan;55(1):69–72. doi: 10.1104/pp.55.1.69

Citrate Cleavage Enzymes from Developing Soybean Cotyledons

Incorporation of Citrate Carbon into Fatty Acids 1

Daniel R Nelson a, Robert W Rinne a
PMCID: PMC541552  PMID: 16659030

Abstract

Data are presented which demonstrate a citrate cleavage enzyme in the supernatant of a developing soybean (Glycine max L. Merr., var. Harosoy 63) cotyledon homogenate following a 126,000g spin for 2 hours. Activity of the enzyme was observed directly in the supernatant enzyme preparation and in a desalted supernatant preparation by measuring the formation of acetylhydroxamate. Acetylhydroxamate production was dependent on citrate and coenzyme A. The reaction increased with time, citrate, and coenzyme A concentrations.

Involvement of the enzyme in lipid synthesis was investigated by the incorporation of carbon from citrate-1,5-14C into fatty acids. Incorporation shows a pH optimum at 8.5, a temperature optimum at 30 C, and a dependence on ATP and coenzyme A. The reaction is linear throughout the range of extract concentrations tested and is linear as a function of time for 1 hour. Isotope was distributed primarily in unsaturated fatty acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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