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. 1975 Feb;55(2):183–186. doi: 10.1104/pp.55.2.183

Partial Reactions of Photosynthesis in Briefly Sonicated Chlamydomonas

I. Cell Breakage and Electron Transport Activities 1

Virginia A Curtis a, Jerry J Brand a, Robert K Togasaki a
PMCID: PMC541580  PMID: 16659047

Abstract

The cell structure of Chlamydomonas reinhardi is disrupted by brief exposure to sonication. The extent of cell breakage can be determined quickly by cell count with the light microscope. Rates of photochemical activities of briefly sonicated cells approach those reported for higher plant chloroplasts. These activities are a sensitive function of time of sonication and sonic power used. The method of brief sonication is rapid and convenient and gives a stable preparation useful for determining photochemical activities in Chlamydomonas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brand J. J., Curtis V. A., Togasaki R. K., Pietro A. S. Partial Reactions of Photosynthesis in Briefly Sonicated Chlamydomonas: II. Photophosphorylation Activities. Plant Physiol. 1975 Feb;55(2):187–191. doi: 10.1104/pp.55.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Curtis V. A., Siedow J. N., San Pietro A. Studies on photosystem I. II. Involvement of ferredoxin in cyclic electron flow. Arch Biochem Biophys. 1973 Oct;158(2):898–902. doi: 10.1016/0003-9861(73)90584-5. [DOI] [PubMed] [Google Scholar]
  4. GRESSEL J., AVRON M. THE EFFECTS OF STRUCTURAL DEGRADATION ON THE COUPLED PHOTOCHEMICAL ACTIVITIES OF ISOLATED CHLOROPLASTS. Biochim Biophys Acta. 1965 Jan 25;94:31–41. doi: 10.1016/0926-6585(65)90005-1. [DOI] [PubMed] [Google Scholar]
  5. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Levine R. P., Goodenough U. W. The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet. 1970;4:397–408. doi: 10.1146/annurev.ge.04.120170.002145. [DOI] [PubMed] [Google Scholar]
  7. Wallach D., Bar-Nun S., Ohad I. Biogenesis of chloroplast membranes. IX. Development of photophosphorylation and proton pump activities in greening Chlamydomonas reinhardi y-I as measured with an open-cell preparation. Biochim Biophys Acta. 1972 Apr 20;267(1):125–137. doi: 10.1016/0005-2728(72)90144-2. [DOI] [PubMed] [Google Scholar]

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