Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1975 Feb;55(2):226–230. doi: 10.1104/pp.55.2.226

Localization and Properties of Ribulose Diphosphate Carboxylase from Castor Bean Endosperm 1

C Barry Osmond a,2, Takashi Akazawa a,3, Harry Beevers a
PMCID: PMC541589  PMID: 16659056

Abstract

A substantial portion of the ribulose 1,5-diphosphate carboxylase activity in the endosperm of germinating castor beans (Ricinus communis var. Hale) is recovered in the proplastid fraction. The partially purified enzyme shows homology with the enzyme from spinach (Spinacia oleracea) leaves, as evidenced by its reaction against antibodies to the native spinach enzyme and to its catalytic subunit. The enzyme from the endosperm of castor beans has a molecular weight of about 500,000 and, with the exception of a higher affinity for ribulose 1,5-diphosphate, has similar kinetic properties to the spinach enzyme. The castor bean carboxylase is inhibited by oxygen and also displays ribulose 1,5-diphosphate oxygenase activity with an optimum at pH 7.5.

Full text

PDF
228

Images in this article

229Image
on p.229

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews T. J., Lorimer G. H., Tolbert N. E. Ribulose diphosphate oxygenase. I. Synthesis of phosphoglycolate by fraction-1 protein of leaves. Biochemistry. 1973 Jan 2;12(1):11–18. doi: 10.1021/bi00725a003. [DOI] [PubMed] [Google Scholar]
  2. Beevers H. Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann N Y Acad Sci. 1969 Dec 19;168(2):313–324. doi: 10.1111/j.1749-6632.1969.tb43118.x. [DOI] [PubMed] [Google Scholar]
  3. Benedict C. R., Beevers H. Formation of sucrose from malate in germinating castor beans. I. Conversion of malate to phosphoenol-pyruvate. Plant Physiol. 1961 Sep;36(5):540–544. doi: 10.1104/pp.36.5.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benedict C. R. The presence of ribulose 1,5-diphosphate carboxylase in the nonphotosynthetic endosperm of germinating castor beans. Plant Physiol. 1973 Apr;51(4):755–759. doi: 10.1104/pp.51.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bowes G., Ogren W. L., Hageman R. H. Phosphoglycolate production catalyzed by ribulose diphosphate carboxylase. Biochem Biophys Res Commun. 1971 Nov 5;45(3):716–722. doi: 10.1016/0006-291x(71)90475-x. [DOI] [PubMed] [Google Scholar]
  6. Bowes G., Ogren W. L. Oxygen inhibition and other properties of soybean ribulose 1,5-diphosphate carboxylase. J Biol Chem. 1972 Apr 10;247(7):2171–2176. [PubMed] [Google Scholar]
  7. Goldthwaite J. J., Bogorad L. A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase. Anal Biochem. 1971 May;41(1):57–66. doi: 10.1016/0003-2697(71)90191-6. [DOI] [PubMed] [Google Scholar]
  8. Hedrick J. L., Smith A. J. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Arch Biochem Biophys. 1968 Jul;126(1):155–164. doi: 10.1016/0003-9861(68)90569-9. [DOI] [PubMed] [Google Scholar]
  9. Huang A. H., Beevers H. Localization of enzymes within microbodies. J Cell Biol. 1973 Aug;58(2):379–389. doi: 10.1083/jcb.58.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Takabe T., Akazawa T. Oxidative formation of phosphoglycolate from ribulose-1,5-diphosphate catalysed by Chromatium ribulose-1,5-diphosphate carboxylase. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1173–1179. doi: 10.1016/0006-291x(73)90588-3. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES