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. 1990 Jun;87(12):4590–4594. doi: 10.1073/pnas.87.12.4590

Regulation of the class II-associated invariant chain gene in normal and mutant B lymphocytes.

C Doyle 1, P J Ford 1, P D Ponath 1, T Spies 1, J L Strominger 1
PMCID: PMC54162  PMID: 2112745

Abstract

The invariant chain protein is intracellularly associated with class II major histocompatibility proteins. In many cases, the expression of these molecules appears to be regulated in a similar manner. Contained within the promoter of the invariant chain gene are sequences (X and I gamma 1) that are similar to the X and Y box elements of class II genes, suggesting that these sequences might be involved in its regulation. DNase I footprinting reveals additional cis-acting elements (I gamma 2 and I gamma 3) that contain sequence similarities to NF-kappa B and/or H2TF1/KBF1 recognition sequences. A series of fusion constructs with the chloramphenicol acetyltransferase reporter gene were used to analyze the role of these sequences (I gamma 1, I gamma 2, I gamma 3, and X and Y elements) in both normal and mutant B lymphocytes. These data suggest the likelihood of multiple X box proteins in B cells, which can act as both negative and positive regulatory factors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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