Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1975 Apr;55(4):768–773. doi: 10.1104/pp.55.4.768

Partial Restoration of the High Rate of Plastid Pigment Development and the Ultrastructure of Plastids in Detached Water-stressed Wheat Leaves 1

Murray E Duysen a, Thomas P Freeman a
PMCID: PMC541703  PMID: 16659164

Abstract

Detached etiolated wheat (Triticum aestivum L. cv. Chris) leaves accumulated plastid pigments at a high rate, developed chloroplasts with stacked thylakoids, and stored plastid starch when wetted on filter paper in light. A moderate water deficit of — 10 bars markedly reduced the accumulation of chlorophyll and carotenoids in the 8-day-old detached leaves during greening. δ-Aminolevulinic acid treatment of stressed leaf segments resulted in slightly increased pigment accumulations but benzyladenine application restored plastid pigment formation in stressed tissue to within 15% of the pigment content of the nonstressed detached leaves. The addition of δ-aminolevulinic acid to benzyladenine-treated stressed leaf segments improved both chlorophyll and carotenoid formation to nearly the amounts found in nonstressed leaf tissue. Stressed leaf sections developed plastids that were small, lacked starch, contained few thylakoids per granum, and possessed dilated thylakoids. Benzyladenine application to the stressed leaf segments did not restore normal plastid stacking but benzyladenine induced the formation of extended intergranal lamellae and stimulated pigment accumulations in both stressed and nonstressed detached leaves. Starch was absent in plastids of benzyladeninetreated leaf sections.

Full text

PDF
770

Images in this article

771Image
on p.771

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Zioni A., Itai C., Vaadia Y. Water and salt stresses, kinetin and protein synthesis in tobacco leaves. Plant Physiol. 1967 Mar;42(3):361–365. doi: 10.1104/pp.42.3.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berridge M. V., Ralph R. K. Kinetin and carbohydrate metabolism in chinese cabbage. Plant Physiol. 1971 Apr;47(4):562–567. doi: 10.1104/pp.47.4.562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boasson R., Laetsch W. M. Chloroplast replication and growth in tobacco. Science. 1969 Nov 7;166(3906):749–751. doi: 10.1126/science.166.3906.749. [DOI] [PubMed] [Google Scholar]
  4. Bourque D. P., Naylor A. W. Large Effects of Small Water Deficits on Chlorophyll Accumulation and Ribonucleic Acid Synthesis in Etiolated Leaves of Jack Bean (Canavalia ensiformis [L.] DC.). Plant Physiol. 1971 Apr;47(4):591–594. doi: 10.1104/pp.47.4.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CRELIN E. S. Mitosis in adult cartilage. Science. 1957 Apr 5;125(3249):650–650. doi: 10.1126/science.125.3249.650. [DOI] [PubMed] [Google Scholar]
  6. Hsiao T. C. Rapid Changes in Levels of Polyribosomes in Zea mays in Response to Water Stress. Plant Physiol. 1970 Aug;46(2):281–285. doi: 10.1104/pp.46.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mlodzianowski F., Kwintkiewicz M. The inhibition of kohlrabi chloroplast degeneration by kinetin. Protoplasma. 1973;76(2):211–226. doi: 10.1007/BF01280698. [DOI] [PubMed] [Google Scholar]
  8. Nadler K., Granick S. Controls on chlorophyll synthesis in barley. Plant Physiol. 1970 Aug;46(2):240–246. doi: 10.1104/pp.46.2.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Remy R. Pre-existence of chloroplast lamellar proteins in wheat etioplasts. Functional and protein changes during greening under continuous or intermittent light. FEBS Lett. 1973 May 1;31(3):308–312. doi: 10.1016/0014-5793(73)80128-0. [DOI] [PubMed] [Google Scholar]
  10. Wolf F. T. Effects of Light and Darkness on Biosynthesis of Carotenoid Pigments in Wheat Seedlings. Plant Physiol. 1963 Nov;38(6):649–652. doi: 10.1104/pp.38.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES