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. 1975 May;55(5):822–827. doi: 10.1104/pp.55.5.822

Phytochrome-mediated de Novo Synthesis of Phenylalanine Ammonia-Lyase in Cell Suspension Cultures of Parsley 1

Eckard Wellmann a, Peter Schopfer a
PMCID: PMC541717  PMID: 16659175

Abstract

After a preirradiation with ultraviolet light, phenylalanine ammonia-lyase activity in cell suspension cultures of parsley (Petroselinum hortense Hoff.) is controlled by phytochrome (red/far red photoreversibility). Isopycnic CsCl density gradient centrifugation, after labeling with 15N (90 atom%) under inductive and noninductive conditions, was used to investigate the mode of action of phytochrome in this response. After a 5hour labeling period, a buoyant density shift of 0.009 kg·l−1 (0.7%) without band-broadening (indicating close to maximal labeling of the enzyme), was observed in irradiated cells. In dark-grown controls, the density shift was 0.004 kg·l−1 (0.3%), accompanied by significant band-broadening, indicating turnover of about half of the enzyme pool during 5 hours. These results are taken as evidence that phytochrome controls de novo synthesis of this enzyme over a background of basal turnover.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acton G. J., Schopfer P. Phytochrome-induced synthesis of ribonuclease de novo in lupin hypocotyl sections. Biochem J. 1974 Sep;142(3):449–455. doi: 10.1042/bj1420449a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Attridge T. H., Johnson C. B., Smith H. Density-labelling evidence for the phytochrome-mediated activation of phenylalanine ammonia-lyase in mustard cotyledons. Biochim Biophys Acta. 1974 May 24;343(3):440–451. doi: 10.1016/0304-4165(74)90261-x. [DOI] [PubMed] [Google Scholar]
  3. Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  5. HU A. S., BOCK R. M., HALVORSON H. O. Separation of labeled from unlabeled proteins by equilibrium density gradient sedimentation. Anal Biochem. 1962 Dec;4:489–504. doi: 10.1016/0003-2697(62)90129-x. [DOI] [PubMed] [Google Scholar]
  6. Hahlbrock K., Ebel J., Ortmann R., Sutter A., Wellmann E., Grisebach H. Regulation of enzyme activities related to the biosynthesis of flavone glycosides in cell suspension cultures of parsley (Petroselinum hortense). Biochim Biophys Acta. 1971 Jul 20;244(1):7–15. doi: 10.1016/0304-4165(71)90114-0. [DOI] [PubMed] [Google Scholar]
  7. Hahlbrock K., Schröder J. Light-induced changes of enzyme activities in parsley cell suspension cultures. Increased rate of synthesis of phenylalanine ammonia-lyase. Arch Biochem Biophys. 1975 Jan;166(1):47–53. doi: 10.1016/0003-9861(75)90363-x. [DOI] [PubMed] [Google Scholar]
  8. Hahlbrock K., Wellmann E. Light-independent induction of enzymes related to phenylpropanoid metabolism in cell suspension cultures from parsley. Biochim Biophys Acta. 1973 May 28;304(3):702–706. doi: 10.1016/0304-4165(73)90215-8. [DOI] [PubMed] [Google Scholar]
  9. Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schimke R. T., Doyle D. Control of enzyme levels in animal tissues. Annu Rev Biochem. 1970;39:929–976. doi: 10.1146/annurev.bi.39.070170.004433. [DOI] [PubMed] [Google Scholar]
  11. Schopfer P., Mohr H. Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions. Plant Physiol. 1972 Jan;49(1):8–10. doi: 10.1104/pp.49.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zielke H. R., Filner P. Synthesis and turnover of nitrate reductase induced by nitrate in cultured tobacco cells. J Biol Chem. 1971 Mar 25;246(6):1772–1779. [PubMed] [Google Scholar]
  13. Zucker M. Induction of Phenylalanine Deaminase by Light and its Relation to Chlorogenic Acid Synthesis in Potato Tuber Tissue. Plant Physiol. 1965 Sep;40(5):779–784. doi: 10.1104/pp.40.5.779. [DOI] [PMC free article] [PubMed] [Google Scholar]

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