Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1975 Jun;55(6):1038–1042. doi: 10.1104/pp.55.6.1038

Galactolipid Synthesis in Vicia faba Leaves

I. Galactose, Glycerol, and Fatty Acid Labeling after 14CO2 Feeding 1

John P Williams a, Garry R Watson a, Mobashsher-Uddin Khan a,2, Stephen Leung a
PMCID: PMC541761  PMID: 16659205

Abstract

The galactose, glycerol, and fatty acids of mono- and digalactosyl diglycerides (MGDG and DGDG) have been separated and analyzed for 14C activity after 14CO2 feeding of Vicia faba leaf discs. Fully expanded and developing leaves were analyzed at time intervals following feeding during continuous illumination. In addition, fully expanded leaves were analyzed after similar times in complete darkness. In all cases, 14C was incorporated very rapidly into galactose, whereas glycerol and fatty acids were labeled much more slowly and over a longer period of time. The data are consistent with the galactosylation of a diglyceride to MGDG which is in turn galactosylated to DGDG. The data suggest that the formation of diglycerides suitable for galactosylation to MGDG is slow in comparison to the galactosylation process. It is also suggested that DGDG may be formed from more than one pool of MGDG. The complete analysis of the 14C incorporation into galactose appears to represent the only satisfactory method of comparing galactolipid synthesis by 14C incorporation. Estimates of comparative rates of synthesis of MGDG and DGDG have been made on this basis.

Full text

PDF
1041

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FERRARI R. A., BENSON A. A. The path of carbon in photosynthesis of the lipids. Arch Biochem Biophys. 1961 May;93:185–192. doi: 10.1016/0003-9861(61)90248-x. [DOI] [PubMed] [Google Scholar]
  3. Neufeld E. F., Hall C. W. Formation of galactolipids by chloroplasts. Biochem Biophys Res Commun. 1964;14:503–508. doi: 10.1016/0006-291x(64)90259-1. [DOI] [PubMed] [Google Scholar]
  4. Ongun A., Mudd J. B. Biosynthesis of galactolipids in plants. J Biol Chem. 1968 Apr 10;243(7):1558–1566. [PubMed] [Google Scholar]
  5. Williams J. P., Khan M., Leung S. Biosynthesis of digalactosyl diglyceride in Vicia faba leaves. J Lipid Res. 1975 Jan;16(1):61–66. [PubMed] [Google Scholar]
  6. Williams J. P., Merrilees P. A. The removal of water and nonlipid contaminants from lipid extracts. Lipids. 1970 Apr;5(4):367–370. doi: 10.1007/BF02532100. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES