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Plant Physiology logoLink to Plant Physiology
. 1976 Jan;57(1):59–62. doi: 10.1104/pp.57.1.59

Nitrate Absorption by Barley

II. Influence of Nitrate Reductase Activity 1

K Prasad Rao a, D William Rains a
PMCID: PMC541963  PMID: 16659426

Abstract

The influence of protein synthesis and nitrate reductase activity on nitrate absorption by barley (Hordeum vulgare L.) was investigated. Cycloheximide decreased nitrate absorption. Pretreatment studies showed that cycloheximide affects either energy transfer or nitrate reductase activity or both.

Illumination increased plant capacity for nitrate absorption, possibly through increased energy supply and/or increased nitrate reductase activity. There was a positive correlation between nitrate reductase activity and light. Inhibiting the development of nitrate reductase activity by tungstate decreased nitrate absorption.

At least two nitrate transport systems are thus proposed in barley: one operating in the dark, with little nitrate reductase activity detectable; and one closely correlated with nitrate reductase activity. Total absorption is the sum of dark absorption and absorption facilitated by nitrate reductase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beevers L., Schrader L. E., Flesher D., Hageman R. H. The Role of Light and Nitrate in the Induction of Nitrate Reductase in Radish Cotyledons and Maize Seedlings. Plant Physiol. 1965 Jul;40(4):691–698. doi: 10.1104/pp.40.4.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ellis R. J., Macdonald I. R. Specificity of cycloheximide in higher plant systems. Plant Physiol. 1970 Aug;46(2):227–232. doi: 10.1104/pp.46.2.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hageman R. H., Flesher D. Nitrate Reductase Activity in Corn Seedlings as Affected by Light and Nitrate Content of Nutrient Media. Plant Physiol. 1960 Sep;35(5):700–708. doi: 10.1104/pp.35.5.700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heimer Y. M., Filner P. Regulation of the nitrate assimilation pathway in cultured tobacco cells. 3. The nitrate uptake system. Biochim Biophys Acta. 1971 Feb 23;230(2):362–372. doi: 10.1016/0304-4165(71)90223-6. [DOI] [PubMed] [Google Scholar]
  5. Heimer Y. M., Wray J. L., Filner P. The effect of tungstate on nitrate assimilation in higher plant tissues. Plant Physiol. 1969 Aug;44(8):1197–1199. doi: 10.1104/pp.44.8.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jackson W. A., Flesher D., Hageman R. H. Nitrate Uptake by Dark-grown Corn Seedlings: Some Characteristics of Apparent Induction. Plant Physiol. 1973 Jan;51(1):120–127. doi: 10.1104/pp.51.1.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ordin L., Jacobson L. Inhibition of Ion Absorption and Respiration in Barley Roots. Plant Physiol. 1955 Jan;30(1):21–27. doi: 10.1104/pp.30.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rains D. W. Kinetics and Energetics of Light-enhanced Potassium Absorption by Corn Leaf Tissue. Plant Physiol. 1968 Mar;43(3):394–400. doi: 10.1104/pp.43.3.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rao K. P., Rains D. W. Nitrate absorption by barley: I. Kinetics and energetics. Plant Physiol. 1976 Jan;57(1):55–58. doi: 10.1104/pp.57.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schloemen R. H., Garrett R. H. Nitrate transport system in Neurospora crassa. J Bacteriol. 1974 Apr;118(1):259–269. doi: 10.1128/jb.118.1.259-269.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schrader L. E., Ritenour G. L., Eilrich G. L., Hageman R. H. Some characteristics of nitrate reductase from higher plants. Plant Physiol. 1968 Jun;43(6):930–940. doi: 10.1104/pp.43.6.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shen T. C. The induction of nitrate reductase and the preferential assimilation of ammonium in germinating rice seedlings. Plant Physiol. 1969 Nov;44(11):1650–1655. doi: 10.1104/pp.44.11.1650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wray J. L., Filner P. Structural and functional relationships of enzyme activities induced by nitrate in barley. Biochem J. 1970 Oct;119(4):715–725. doi: 10.1042/bj1190715. [DOI] [PMC free article] [PubMed] [Google Scholar]

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