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. 1976 Jan;57(1):88–92. doi: 10.1104/pp.57.1.88

Stimulation of Ethylene Production in the Mung Bean Hypocotyls by Cupric Ion, Calcium Ion, and Kinetin 1

Oi-Lim Lau a, Shang F Yang a
PMCID: PMC541969  PMID: 16659432

Abstract

The synergistic stimulation of ethylene production by kinetin and Ca2+ in hypocotyl segments of mung bean (Phaseolus aureus Roxb.) seedling was further studied. The requirement for Ca2+ in this system was specific. Except for Sr2+, which mimicked the effect of Ca2+, none of the following divalent cations, including Ba2+, Mg6+, Cu2+, Hg2+, Co2+, Ni2+, Sn2+, and Zn2+, showed synergism with kinetin on ethylene production. Fe2+, however, showed a slight synergism with kinetin. Some of them (Hg2+, Co2+, and Ni2+) had a strong inhibitory effect, while others (Zn2+, Mg2+, Sn2+, and Ba2+) had a slight or no inhibitory effect on ethylene production in the absence or presence of kinetin.

Cu2+ alone, depending on the concentration applied, stimulated ethylene production with a lag period of about 2 hours and had no synergism with kinetin on ethylene production. When Cu2+ was applied with Ca2+, a remarkable synergistic stimulation of ethylene production was observed. Tracer experiments indicated that Cu2+ enhanced the uptake of 45Ca2+ into the tissues during the first few hours of incubation, and this increase of 45Ca2+ uptake paralleled the enhancement of ethylene production. When Ca2+ was applied together with kinetin plus Cu2+, both the ethylene production and the 45Ca2+ uptake were greatly increased over those from the segments treated with Cu2+ or kinetin alone. The increase in ethylene production as a result of kinetin plus Ca2+ plus Cu2+ treatment is equal to the combined increases caused by kinetin plus Ca2+ and Cu2+ plus Ca2+. A possible mechanism accounting for such cooperative effects of Cu2+, Ca2+, and kinetin on ethylene production is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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