Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1976 Mar;57(3):458–459. doi: 10.1104/pp.57.3.458

Nitrate Reductase in Barley Roots under Sterile, Low Oxygen Conditions 1

Dale G Blevins a,2, Richie H Lowe a, Lana Staples a
PMCID: PMC542047  PMID: 16659504

Abstract

Levels of nitrate reductase activity (EC 1.9.6.1.) as high as 11 μmoles nitrite produced/hour gram fresh weight were found in barley (Hordeum vulgare cv. Compana) roots grown under low oxygen conditions. Roots of plants given identical treatment under sterile conditions did not develop the high levels of nitrate reductase activity. The results suggest that the buildup of particulate, reduced viologen-utilizing nitrate reductase reported in barley roots may be caused by bacterial contamination. The nitrate reductase activity in roots grown under low oxygen conditions was not specific for reduced nicotinamide adenine dinucleotide like the assimilatory nitrate reductase (EC 1.6.6.1.) normally found in aerated plant roots.

Full text

PDF
459

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blevins D. G., Hiatt A. J., Lowe R. H. The Influence of Nitrate and Chloride Uptake on Expressed Sap pH, Organic Acid Synthesis, and Potassium Accumulation in Higher Plants. Plant Physiol. 1974 Jul;54(1):82–87. doi: 10.1104/pp.54.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ferrari T. E., Varner J. E. Control of nitrate reductase activity in barley aleurone layers. Proc Natl Acad Sci U S A. 1970 Mar;65(3):729–736. doi: 10.1073/pnas.65.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hageman R. H., Flesher D. Nitrate Reductase Activity in Corn Seedlings as Affected by Light and Nitrate Content of Nutrient Media. Plant Physiol. 1960 Sep;35(5):700–708. doi: 10.1104/pp.35.5.700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lester R. L., DeMoss J. A. Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli. J Bacteriol. 1971 Mar;105(3):1006–1014. doi: 10.1128/jb.105.3.1006-1014.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sanderson G. W., Cocking E. C. Enzymic Assimilation of Nitrate in Tomato Plants. I. Reduction of Nitrate to Nitrite. Plant Physiol. 1964 May;39(3):416–422. doi: 10.1104/pp.39.3.416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. TANIGUCHI S., ITAGAKI E. Nitrate reductase of nitrate respiration type from E. coli. I. Solubilization and purification from the particulate system with molecular characterization as a metalloprotein. Biochim Biophys Acta. 1960 Nov 4;44:263–279. doi: 10.1016/0006-3002(60)91562-6. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES