Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1976 Sep;58(3):292–294. doi: 10.1104/pp.58.3.292

NADH- and NAD(P)H-Nitrate Reductases in Rice Seedlings

Teh-Chien Shen a,1, Edward A Funkhouser a, Miguel G Guerrero a
PMCID: PMC542233  PMID: 16659665

Abstract

By use of affinity chromatography on blue dextran-Sepharose, two nitrate reductases from rice (Oryza sativa L.) seedlings, specifically, NADH:nitrate oxidoreductase (EC 1.6.6.1) and NAD(P)-H:nitrate oxidoreductase (EC 1.6.6.2), have been partially separated. Nitrate-induced seedlings contained more NADH-nitrate reductase than NAD(P)H-nitrate reductase, whereas chloramphenicol-induced seedlings contained primarily NAD(P)H-nitrate reductase. NAD(P)H-nitrate reductase was shown to utilize NADPH directly as reductant. This enzyme has a preference for NADPH, but reacts about half as well with NADH.

Full text

PDF
293

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Kadam S. S., Gandhi A. P., Sawhney S. K., Naik M. S. Inhibitor of nitrate reductase in the roots of rice seedlings and its effect on the enzyme activity in the presence of NADH. Biochim Biophys Acta. 1974 May 20;350(1):162–170. doi: 10.1016/0005-2744(74)90214-9. [DOI] [PubMed] [Google Scholar]
  2. Ryan L. D., Vestling C. S. Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach. Arch Biochem Biophys. 1974 Jan;160(1):279–284. doi: 10.1016/s0003-9861(74)80035-4. [DOI] [PubMed] [Google Scholar]
  3. Shen T. C. Nitrate reductase of rice seedlings and its induction by organic nitro-compounds. Plant Physiol. 1972 Apr;49(4):546–549. doi: 10.1104/pp.49.4.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Shen T. C. The induction of nitrate reductase and the preferential assimilation of ammonium in germinating rice seedlings. Plant Physiol. 1969 Nov;44(11):1650–1655. doi: 10.1104/pp.44.11.1650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Solomonson L. P. Purification of NADH-Nitrate Reductase by Affinity Chromatography. Plant Physiol. 1975 Dec;56(6):853–855. doi: 10.1104/pp.56.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Wells G. N., Hageman R. H. Specificity for nicotinamide adenine dinucleotide by nitrate reductase from leaves. Plant Physiol. 1974 Aug;54(2):136–141. doi: 10.1104/pp.54.2.136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wray J. L., Filner P. Structural and functional relationships of enzyme activities induced by nitrate in barley. Biochem J. 1970 Oct;119(4):715–725. doi: 10.1042/bj1190715. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES