Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1976 Nov;58(5):614–617. doi: 10.1104/pp.58.5.614

Selective Modulation of RNA Polymerase I Activity during Growth Transitions in the Soybean Seedling

Chu-Yung Lin a,1, Yih-Ming Chen a,1, Tom J Guilfoyle a, Joe L Key a
PMCID: PMC542268  PMID: 16659729

Abstract

RNA polymerase I and II activities were measured in tissues of the soybean (Glycina max, var. Wayne) hypocotyl where dramatic changes in the relative level of RNA synthesis are associated with normal and auxin-induced growth transitions. When assayed in isolated nuclei, the activity of RNA polymerase I changed much more than the activity of RNA polymerase II during these growth transitions. The activity of RNA polymerase I expressed in the nuclei generally showed a positive correlation with the relative level of RNA synthesis (i.e. accumulation) of that tissue. Following solubilization of the RNA polymerases from these isolated nuclei and fractionation of them on DEAE-cellulose, the activity of RNA polymerase I relative to that of RNA polymerase II showed smaller changes during these growth transitions than when assayed in the nuclei. Thus, these data indicate that the activity of RNA polymerase I is significantly modulated in the nucleus, up or down depending upon the growth state, during growth transitions in the soybean in addition to lesser changes which occur in the apparent level of the enzyme.

Full text

PDF
614

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Block R., Haseltine A. W. Purification and properties of stringent factor. J Biol Chem. 1975 Feb 25;250(4):1212–1217. [PubMed] [Google Scholar]
  2. Cashel M. Regulation of bacterial ppGpp and pppGpp. Annu Rev Microbiol. 1975;29:301–318. doi: 10.1146/annurev.mi.29.100175.001505. [DOI] [PubMed] [Google Scholar]
  3. Chen Y. M., Lin C. Y., Chang H., Guilfoyle T. J., Key J. L. Isolation and Properties of Nuclei from Control and Auxin-treated Soybean Hypocotyl. Plant Physiol. 1975 Jul;56(1):78–82. doi: 10.1104/pp.56.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edlin G., Broda P. Physiology and genetics of the "ribonucleic acid control" locus in escherichia coli. Bacteriol Rev. 1968 Sep;32(3):206–226. doi: 10.1128/br.32.3.206-226.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fuhrman S. A., Gill G. N. Adrenocorticotropic hormone stimulation of adrenal RNA polymerase I and III activities. Nucleotide incorporation into internal positions and 3' chain termini. Biochemistry. 1975 Jul;14(13):2925–2933. doi: 10.1021/bi00684a021. [DOI] [PubMed] [Google Scholar]
  6. Gross K. J., Pogo A. O. Control mechanism of ribonucleic acid synthesis in eukaryotes. The effect of amino acid and glucose starvation and cycloheximide on yeast deoxyribonucleic acid-dependent ribonucleic acid polymerases. J Biol Chem. 1974 Jan 25;249(2):568–576. [PubMed] [Google Scholar]
  7. Guilfoyle T. J., Hanson J. B. Increased Activity of Chromatin-bound Ribonucleic Acid Polymerase from Soybean Hypocotyl with Spermidine and High Ionic Strength. Plant Physiol. 1973 Jun;51(6):1022–1025. doi: 10.1104/pp.51.6.1022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guilfoyle T. J., Lin C. Y., Chen Y. M., Key J. L. Purification and characterization of RNA polymerase I from a higher plant. Biochim Biophys Acta. 1976 Feb 5;418(3):344–357. doi: 10.1016/0005-2787(76)90296-3. [DOI] [PubMed] [Google Scholar]
  9. Guilfoyle T. J., Lin C. Y., Chen Y. M., Nagao R. T., Key J. L. Enhancement of soybean RNA polymerase I by auxin. Proc Natl Acad Sci U S A. 1975 Jan;72(1):69–72. doi: 10.1073/pnas.72.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hardin J. W., Cherry J. H. Solubilization and partial characterization of soybean chromatin-bound RNA polymerase. Biochem Biophys Res Commun. 1972 Jul 25;48(2):299–306. doi: 10.1016/s0006-291x(72)80050-0. [DOI] [PubMed] [Google Scholar]
  11. Holm R. E., O'brien T. J., Key J. L., Cherry J. H. The Influence of Auxin and Ethylene on Chromatin-directed Ribonucleic Acid Synthesis in Soybean Hypocotyl. Plant Physiol. 1970 Jan;45(1):41–45. doi: 10.1104/pp.45.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jaehning J. A., Stewart C. C., Roeder R. G. DNA-dependent RNA polymerase levels during the response of human peripheral lymphocytes to phytohemagglutinin. Cell. 1975 Jan;4(1):51–57. doi: 10.1016/0092-8674(75)90133-6. [DOI] [PubMed] [Google Scholar]
  13. Key J. L. Ribonucleic Acid and Protein Synthesis as Essential Processes for Cell Elongation. Plant Physiol. 1964 May;39(3):365–370. doi: 10.1104/pp.39.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Key J. L., Shannon J. C. Enhancement by Auxin of Ribonucleic Acid Synthesis in Excised Soybean Hypocotyl Tissue. Plant Physiol. 1964 May;39(3):360–364. doi: 10.1104/pp.39.3.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lin C. Y., Guilfoyle T. J., Chen Y. M., Key J. L. Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl. Plant Physiol. 1975 Dec;56(6):850–852. doi: 10.1104/pp.56.6.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lin C. Y., Guilfoyle T. J., Chen Y. M., Nagao R. T., Key J. L. The separation of RNA polymerases I and II achieved by fractionation of plant chromatin. Biochem Biophys Res Commun. 1974 Sep 23;60(2):498–506. doi: 10.1016/0006-291x(74)90268-x. [DOI] [PubMed] [Google Scholar]
  17. Lindell T. J., Weinberg F., Morris P. W., Roeder R. G., Rutter W. J. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science. 1970 Oct 23;170(3956):447–449. doi: 10.1126/science.170.3956.447. [DOI] [PubMed] [Google Scholar]
  18. Mamont P., Hershko A., Kram R., Schacter L., Lust J., Tomkins G. M. The pleiotypic response in mammalian cells: search for an intracellular mediator. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1378–1384. doi: 10.1016/0006-291x(72)90865-0. [DOI] [PubMed] [Google Scholar]
  19. Noodén L. D., Thimann K. V. EVIDENCE FOR A REQUIREMENT FOR PROTEIN SYNTHESIS FOR AUXIN-INDUCED CELL ENLARGEMENT. Proc Natl Acad Sci U S A. 1963 Aug;50(2):194–200. doi: 10.1073/pnas.50.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. O'Brien T. J., Jarvis B. C., Cherry J. H., Hanson J. B. Enhancement by 2,4-dichlorophenoxyacetic acid of chromatin RNA polymerase in soybean hypocotyl tissue. Biochim Biophys Acta. 1968 Nov 20;169(1):35–43. doi: 10.1016/0005-2787(68)90006-3. [DOI] [PubMed] [Google Scholar]
  21. Reeder R. H., Roeder R. G. Ribosomal RNA synthesis in isolated nuclei. J Mol Biol. 1972 Jun 28;67(3):433–441. doi: 10.1016/0022-2836(72)90461-5. [DOI] [PubMed] [Google Scholar]
  22. Roeder R. G., Rutter W. J. Specific nucleolar and nucleoplasmic RNA polymerases. Proc Natl Acad Sci U S A. 1970 Mar;65(3):675–682. doi: 10.1073/pnas.65.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sebastian J., Mian F., Halvorson H. O. Effect of the growth rate on the level of the DNA-dependent RNA polymerases in Saccharomyces cerevisiae. FEBS Lett. 1973 Aug 15;34(2):159–162. doi: 10.1016/0014-5793(73)80782-3. [DOI] [PubMed] [Google Scholar]
  24. Travis R. L., Anderson J. M., Key J. L. Influence of auxin and incubation on the relative level of polyribosomes in excised soybean hypocotyl. Plant Physiol. 1973 Dec;52(6):608–612. doi: 10.1104/pp.52.6.608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yu F. L., Feigelson P. A proposed model for the glucocorticoidal regulation of rat hepatic ribosomal RNA synthesis. Biochem Biophys Res Commun. 1973 Aug 6;53(3):754–760. doi: 10.1016/0006-291x(73)90157-5. [DOI] [PubMed] [Google Scholar]
  26. van Ooyen A. J., de Boer H. A., Ab G., Gruber M. Specific inhibition of ribosomal RNA synthesis in vitro by guanosine 3' diphosphate, 5' diphosphate. Nature. 1975 Apr 10;254(5500):530–531. doi: 10.1038/254530a0. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES