Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1977 Mar;59(3):369–371. doi: 10.1104/pp.59.3.369

Osmotic Shock Inhibits Auxin-stimulated Acidification and Growth 1

Bernard Rubinstein a
PMCID: PMC542404  PMID: 16659853

Abstract

Cells of oat coleoptiles (Avena sativa L. cv. “Garry”) have been osmotically shocked in order to observe the effect of alterations of the plasma membrane on some auxin responses. When coleoptile sections were treated sequentially with 0.5 m mannitol and 1 mm Na-phosphate (pH 6.4) at 4 C, polar auxin transport and acidification by 1 mM CaCl2 were unaffected, but auxin-stimulated acidification and growth were eliminated. Shock treatment also had no effect on acid-stimulated growth or on freezing point depression by the cytoplasm. It is suggested that osmotic shock modifies a portion of the plasma membrane which interacts with auxin and eventually leads to growth.

Full text

PDF
370

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amar L., Reinhold L. Loss of membrane transport ability in leaf cells and release of protein as a result of osmotic shock. Plant Physiol. 1973 Apr;51(4):620–625. doi: 10.1104/pp.51.4.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cleland R. Auxin-induced hydrogen ion excretion from Avena coleoptiles. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3092–3093. doi: 10.1073/pnas.70.11.3092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dela Fuente R. K., Leopold A. C. Time course of auxin stimulations of growth. Plant Physiol. 1970 Aug;46(2):186–189. doi: 10.1104/pp.46.2.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Etherton B. Effect of Indole-3-acetic Acid on Membrane Potentials of Oat Coleoptile Cells. Plant Physiol. 1970 Apr;45(4):527–528. doi: 10.1104/pp.45.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green P. B., Cummins W. R. Growth rate and turgor pressure: auxin effect studies with an automated apparatus for single coleoptiles. Plant Physiol. 1974 Dec;54(6):863–869. doi: 10.1104/pp.54.6.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haschke H. P., Lüttge U. Stoichiometric Correlation of Malate Accumulation with Auxin-dependent K-H Exchange and Growth in Avena Coleoptile Segments. Plant Physiol. 1975 Nov;56(5):696–698. doi: 10.1104/pp.56.5.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heppel L. A. Selective release of enzymes from bacteria. Science. 1967 Jun 16;156(3781):1451–1455. doi: 10.1126/science.156.3781.1451. [DOI] [PubMed] [Google Scholar]
  8. Jacobs M., Ray P. M. Promotion of Xyloglucan Metabolism by Acid pH. Plant Physiol. 1975 Sep;56(3):373–376. doi: 10.1104/pp.56.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oxender D. L. Membrane transport. Annu Rev Biochem. 1972;41(10):777–814. doi: 10.1146/annurev.bi.41.070172.004021. [DOI] [PubMed] [Google Scholar]
  10. Rubinstein B. Effect of pH and Auxin on Chloride Uptake into Avena Coleoptile Cells. Plant Physiol. 1974 Dec;54(6):835–839. doi: 10.1104/pp.54.6.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rubinstein B., Mahar P. Effects of Osmotic Shock on Some Membrane-regulated Events of Oat Coleoptile Cells. Plant Physiol. 1977 Mar;59(3):365–368. doi: 10.1104/pp.59.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tanada T. Indoleacetic Acid and abscisic Acid antagonism: I. On the phytochrome-mediated attachment of mung bean root tips on glass. Plant Physiol. 1973 Jan;51(1):150–153. doi: 10.1104/pp.51.1.150. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES