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. 1977 Apr;59(4):684–687. doi: 10.1104/pp.59.4.684

Formation of N-Acetylglutamate by Extracts of Higher Plants

Clayton J Morris 1, John F Thompson 1
PMCID: PMC542473  PMID: 16659918

Abstract

The enzymic synthesis of N-acetylglutamate was studied in extracts of higher plant tissues, especially in sugar beet leaves (Beta vulgaris L.). Sugar beet leaves had an enzyme that transferred the acetyl group either from acetyl-CoA or from N2-acetylornithine to glutamate. The enzyme was so unstable that special precautions were necessary for its detection and appreciable purification was impossible. The Km values were 2.5 and 0.025 mM for acetyl-CoA and N2-acetylornithine, respectively. The Km for glutamate was 23 mM with acetylornithine-glutamate transacetylase and 2.7 mM with acetyl-CoA-glutamate transacetylase. The pH optimum for acetyl-CoA-glutamate transacetylase was about 7.2 whereas that for acetylornithine-glutamate transacetylase was about 8.3. Acetylphosphate, N2-acetyl-2,4-diaminobutyrate, propionyl-CoA, and succinyl-CoA were not substrates.

Arginine inhibited the acetyl-CoA-glutamate transacetylase and acetylglutamate phosphokinase but had no effect on the acetylornithineglutamate transacetylase. Related compounds had either no effect or much less than arginine. Arginine had no effect on enzyme levels.

Acetyl-CoA-glutamate transacetylase was also found in Raphanus sativus L., Glycine max L. Merr., Arachis hypogaea L., Brassica rapa L., and Pisum sativum L. Acetylornithine-glutamate transacetylase was found in all of the above species plus Zea mays L., Avena sativa L., and Triticum aestivum L.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DEDEKEN R. H. BIOSYNTH'ESE DE L'ARGININE CHEZ LA LEVURE. I. LE SORT DE LA N-ALPHA-AC'ETYLORINITHINE. Biochim Biophys Acta. 1963 Dec 13;78:606–616. doi: 10.1016/0006-3002(63)91026-6. [DOI] [PubMed] [Google Scholar]
  2. Haas D., Kurer V., Leisinger T. N-acetylglutamate synthetase of Pseudomonas aeruginosa. An assay in vitro and feedback inhibition by arginine. Eur J Biochem. 1972 Dec 4;31(2):290–295. doi: 10.1111/j.1432-1033.1972.tb02531.x. [DOI] [PubMed] [Google Scholar]
  3. Hoare D. S., Hoare S. L. Feedback regulation of arginine biosynthesis in blue-green algae and photosynthetic bacteria. J Bacteriol. 1966 Aug;92(2):375–379. doi: 10.1128/jb.92.2.375-379.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Leisinger T., Haas D. N-Acetylglutamate synthase of Escherichia coli regulation of synthesis and activity by arginine. J Biol Chem. 1975 Mar 10;250(5):1690–1693. [PubMed] [Google Scholar]
  6. Maas W. K., Novelli G. D., Lipmann F. Acetylation of Glutamic Acid by Extracts of Escherichia Coli. Proc Natl Acad Sci U S A. 1953 Oct;39(10):1004–1008. doi: 10.1073/pnas.39.10.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morris C. J., Thompson J. F. Acetyl coenzyme a-glutamate acetyltransferase and N-acetylornithine-glutamate acetyltransferase of chlorella. Plant Physiol. 1975 Jun;55(6):960–967. doi: 10.1104/pp.55.6.960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morris C. J., Thompson J. F. Conversion of m-carboxyphenylalanine to m-carboxyphenylglycine in Wedgewood iris leaves. Arch Biochem Biophys. 1965 Jun;110(3):506–510. doi: 10.1016/0003-9861(65)90443-1. [DOI] [PubMed] [Google Scholar]
  9. Morris C. J., Thompson J. F., Johnson C. M. Metabolism of Glutamic Acid and N-Acetylglutamic Acid in Leaf Discs and Cell-free Extracts of Higher Plants. Plant Physiol. 1969 Jul;44(7):1023–1026. doi: 10.1104/pp.44.7.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Staub M., Dénes G. Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. I. Purification and properties of ornithine acetyltransferase. Biochim Biophys Acta. 1966 Oct 17;128(1):82–91. doi: 10.1016/0926-6593(66)90144-5. [DOI] [PubMed] [Google Scholar]
  11. THOMPSON J. F., GERING R. K. Preparation of N alpha-acetylornithine. Arch Biochem Biophys. 1962 Nov;99:326–327. doi: 10.1016/0003-9861(62)90018-8. [DOI] [PubMed] [Google Scholar]
  12. Udaka S. Pathway-specific pattern of control of arginine biosynthesis in bacteria. J Bacteriol. 1966 Feb;91(2):617–621. doi: 10.1128/jb.91.2.617-621.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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