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. 1977 Apr;59(4):713–718. doi: 10.1104/pp.59.4.713

UDP-glucose: Glucan Synthetase in Developing Cotton Fibers

I. Kinetic and Physiological Properties 1

Deborah P Delmer a,2, Ursula Heiniger a,3, Carl Kulow a
PMCID: PMC542479  PMID: 16659924

Abstract

A uridine diphosphate(UDP)-glucose:glucan synthetase can be demonstrated in detached cotton fibers (Gossypium hirsutum L.) and in an isolated particulate fraction from such fibers. When assayed with detached fibers, the kinetics of the glucan synthetase activity with respect to variation in substrate concentration is complex and indicates activation of the enzyme by the substrate. Activity is stimulated by Ca2+ or Mg2+ and β-linked glucosides; the effect of the β-linked glucosides is to shift the range in which substrate activation occurs to lower concentrations of UDP-glucose. At concentrations of UDP-glucose below 50 μm, addition of uridine triphosphate, in addition to β-linked glucoside, results in significant stimulation of activity. This effect can be explained by the conversion of uridine triphosphate to UDP-glucose by UDP-glucose pyrophosphorylase, thereby raising substrate concentration to the activating range. In detached fibers, glucan synthetase activity is high at all stages of fiber development. The properties of the glucan synthetase of the isolated particulate fraction closely resemble those of the enzyme assayed in detached fibers; however, in contrast to detached fibers, the ability to detect enzyme activity is more dependent on fiber age, showing maximal activity between 16 and 18 days postanthesis, coincident with the time of rapid onset of secondary wall cellulose deposition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chambers J., Elbein A. D. Biosynthesis of glucans in mung bean seedlings. Formation of beta-(1,4)-glucans from GDP-glucose and beta-(1,3)-glucans from UDP-glucose. Arch Biochem Biophys. 1970 Jun;138(2):620–631. doi: 10.1016/0003-9861(70)90389-9. [DOI] [PubMed] [Google Scholar]
  2. Delmer D. P., Albersheim P. The Biosynthesis of Sucrose and Nucleoside Diphosphate Glucoses in Phaseolus aureus. Plant Physiol. 1970 Jun;45(6):782–786. doi: 10.1104/pp.45.6.782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FEINGOLD D. S., NEUFELD E. F., HASSID W. Z. Synthesis of a beta-1, 3-linked glucan by extracts of Phaseolus aureus seedlings. J Biol Chem. 1958 Oct;233(4):783–788. [PubMed] [Google Scholar]
  4. Forsee W. T., Elbein A. D. Biosynthesis of mannosyl- and glucosyl-phosphoryl-polyprenols in cotton fibers. J Biol Chem. 1973 Apr 25;248(8):2858–2867. [PubMed] [Google Scholar]
  5. Heiniger U., Delmer D. P. UDP-glucose: Glucan Synthetase in Developing Cotton Fibers: II. Structure of the Reaction Product. Plant Physiol. 1977 Apr;59(4):719–723. doi: 10.1104/pp.59.4.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kemp J., Loughman B. C. Cyclitol glucosides and their role in the synthesis of a glucan from uridine diphosphate glucose in Phaseolus aureus. Characterization of some cyclitol glucosides and their synthesis. Biochem J. 1974 Jul;142(1):153–159. doi: 10.1042/bj1420153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Péaud-Lenoël C., Axelos M. D-glucosylation des phytostérols et acylation des stéryl-D-glucosides en présence d'enzymes de plantes. Carbohydr Res. 1972 Oct;24(2):247–262. doi: 10.1016/s0008-6215(00)85060-0. [DOI] [PubMed] [Google Scholar]
  8. Péaud-Lenoël C., Axelos M. Structural features of the beta-glucans enzymatically synthesized from uridine diphosphate glucose by wheat seedlings. FEBS Lett. 1970 Jun 8;8(4):224–228. doi: 10.1016/0014-5793(70)80270-8. [DOI] [PubMed] [Google Scholar]
  9. Shore G., Maclachlan G. A. The site of cellulose synthesis. Hormone treatment alters the intracellular location of alkali-insoluble beta-1,4-glucan (cellulose) synthetase activities. J Cell Biol. 1975 Mar;64(3):557–571. doi: 10.1083/jcb.64.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shore G., Raymond Y., Maclachlan G. A. The Site of Cellulose Synthesis: Cell Surface and Intracellular beta-1, 4-Glucan (Cellulose) Synthetase Activities in Relation to the Stage and Direction of Cell Growth. Plant Physiol. 1975 Jul;56(1):34–38. doi: 10.1104/pp.56.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith M. M., Stone B. A. Beta-glucan synthesis by cell-free extracts from Lolium multiflorum endosperm. Biochim Biophys Acta. 1973 Jun 20;313(1):72–94. doi: 10.1016/0304-4165(73)90189-x. [DOI] [PubMed] [Google Scholar]
  12. Southworth D., Dickinson D. B. beta-1, 3-Glucan Synthase from Lilium longiflorum Pollen. Plant Physiol. 1975 Jul;56(1):83–87. doi: 10.1104/pp.56.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tsai C. M., Hassid W. Z. Solubilization and Separation of Uridine Diphospho-d-glucose: beta-(1 --> 4) Glucan and Uridine Diphospho-d-glucose:beta-(1 --> 3) Glucan Glucosyltransferases from Coleoptiles of Avena sativa. Plant Physiol. 1971 Jun;47(6):740–744. doi: 10.1104/pp.47.6.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tsai C. M., Hassid W. Z. Substrate Activation of beta-(1 --> 3) Glucan Synthetase and Its Effect on the Structure of beta-Glucan Obtained from UDP-d-glucose and Particulate Enzyme of Oat Coleoptiles. Plant Physiol. 1973 Jun;51(6):998–1001. doi: 10.1104/pp.51.6.998. [DOI] [PMC free article] [PubMed] [Google Scholar]

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