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. 1979 Mar;63(3):409–415. doi: 10.1104/pp.63.3.409

Importance of Glutamate Synthase in Glutamate Synthesis by Soybean Cell Suspension Cultures 1

Jane Y Chiu a, Peter D Shargool a
PMCID: PMC542841  PMID: 16660738

Abstract

The specific activities of glutamate synthase|EC 2.6.1.53, l-glutamine: α-ketoglutarate amino transferase (NADPH-oxidising)| and glutamine synthetase|EC 6.3.1.2, l-glutamate: ammonia ligase (ADP-forming)| extracted from soybean (Glycine max L.) cells grown in modified B5 medium were found to vary significantly in response to variations in the nitrogen content of the medium. The changes seen in specific activity levels could be correlated with similar patterns seen in the growth of the cells, in response to changes in the nitrogen content of the medium. By contrast, the specific activity of glutamate dehydrogenase|EC 1.4.1.2, l-glutamate: NAD+ oxidoreductase (deaminating)|, was relatively low and invariant. Glutamate synthase was extracted from cells grown under optimal conditions, partially purified, and shown to have many properties in common with preparations of this enzyme extracted from other plant sources. Glutamate synthase was purified to homogeneity, using affinity chromatography on blue Sepharose.

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Selected References

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  1. Beevers L., Storey R. Glutamate Synthetase in Developing Cotyledons of Pisum sativum. Plant Physiol. 1976 Jun;57(6):862–866. doi: 10.1104/pp.57.6.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boland M. J., Benny A. G. Enzymes of nitrogen metabolism in legume nodules. Purification and properties of NADH-dependent glutamate synthase from lupin nodules. Eur J Biochem. 1977 Oct 3;79(2):355–362. doi: 10.1111/j.1432-1033.1977.tb11816.x. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. Dougall D. K. Evidence for the presence of glutamate synthase in extracts of carrot cell cultures. Biochem Biophys Res Commun. 1974 Jun 4;58(3):639–646. doi: 10.1016/s0006-291x(74)80466-3. [DOI] [PubMed] [Google Scholar]
  5. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  6. Gamborg O. L. The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 1970 Apr;45(4):372–375. doi: 10.1104/pp.45.4.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kanamori T., Matsuoto H. Glutamine synthetase from rice plant roots. Arch Biochem Biophys. 1972 Sep;152(1):404–412. doi: 10.1016/0003-9861(72)90230-5. [DOI] [PubMed] [Google Scholar]
  8. Lea P. J., Miflin B. J. The occurrence of glutamate synthase in algae. Biochem Biophys Res Commun. 1975 Jan 2;64(3):856–862. doi: 10.1016/0006-291x(75)90126-6. [DOI] [PubMed] [Google Scholar]
  9. Miflin B. J., Lea P. J. Glutamine and asparagine as nitrogen donors for reductant-dependent glutamate synthesis in pea roots. Biochem J. 1975 Aug;149(2):403–409. doi: 10.1042/bj1490403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shargool P. D. Some aspects of the regulation of arginine biosynthesis in soybean cell cultures. Plant Physiol. 1973 Jul;52(1):68–71. doi: 10.1104/pp.52.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]

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