Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1979 Apr;63(4):669–673. doi: 10.1104/pp.63.4.669

Galactolipid Synthesis in Vicia faba Leaves

III. Site(s) of Galactosyl Transferase Activity 1

John P Williams a, Ellen E Simpson a, David J Chapman a
PMCID: PMC542892  PMID: 16660787

Abstract

Leaves of Vicia faba were fed 14CO2 in light for periods of up to 6 hours. At intervals, leaf samples were homogenized and separated into fractions which contained “broken” and “intact” chloroplasts, and three other high speed centrifugal fractions containing other cell membranes and chloroplast envelopes. Analyses of the radioactive labeling of galactose from the galactolipids in these fractions and in purified chloroplast envelopes indicated that the major site of galactosyl transferase enzyme activity was in the chloroplast envelope. The data suggest that in time much of the radioactive galactolipid was transferred from the envelope to the thylakoid-containing fractions. The major site of galactolipid synthesis appears to be in the envelope but there is some evidence of another site in the thylakoids.

Full text

PDF
671

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Douce R., Holtz R. B., Benson A. A. Isolation and properties of the envelope of spinach chloroplasts. J Biol Chem. 1973 Oct 25;248(20):7215–7222. [PubMed] [Google Scholar]
  3. Douce R. Site of biosynthesis of galactolipids in spinach chloroplasts. Science. 1974 Mar 1;183(4127):852–853. doi: 10.1126/science.183.4127.852. [DOI] [PubMed] [Google Scholar]
  4. Joyard J., Douce R. Mise en évidence et rôle des diacylglycerols de l'enveloppe des chloroplastes d'épinard. Biochim Biophys Acta. 1976 Jan 22;424(1):125–131. doi: 10.1016/0005-2760(76)90057-6. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Mackender R. O., Leech R. M. The Galactolipid, Phospholipid, and Fatty Acid Composition of the Chloroplast Envelope Membranes of Vicia faba. L. Plant Physiol. 1974 Mar;53(3):496–502. doi: 10.1104/pp.53.3.496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McCarty R. E., Douce R., Benson A. A. The acyl lipids of highly purified plant mitochondria. Biochim Biophys Acta. 1973 Aug 23;316(2):266–270. doi: 10.1016/0005-2760(73)90019-2. [DOI] [PubMed] [Google Scholar]
  8. Poincelot R. P., Day P. R. An improved method for the isolation of spinach chloroplast envelope membranes. Plant Physiol. 1974 Nov;54(5):780–783. doi: 10.1104/pp.54.5.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Poincelot R. P. Isolation and lipid composition of spinach chloroplast envelope membranes. Arch Biochem Biophys. 1973 Nov;159(1):134–142. doi: 10.1016/0003-9861(73)90437-2. [DOI] [PubMed] [Google Scholar]
  10. Rocha V., Ting I. P. Preparation of cellular plant organelles from spinach leaves. Arch Biochem Biophys. 1970 Oct;140(2):398–407. doi: 10.1016/0003-9861(70)90081-0. [DOI] [PubMed] [Google Scholar]
  11. Sabnis D. D., Gordon M., Galston A. W. Localization of adenosine triphosphatase activity on the chloroplast envelope in tendrils of Pisum sativum. Plant Physiol. 1970 Jan;45(1):25–32. doi: 10.1104/pp.45.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Slack C. R., Roughan P. G. The kinetics of incorporation in vivo of (14C)acetate and (14C)carbon dioxide into the fatty acids of glycerolipids in developing leaves. Biochem J. 1975 Nov;152(2):217–228. doi: 10.1042/bj1520217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TAUSSKY H. H., SHORR E. A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953 Jun;202(2):675–685. [PubMed] [Google Scholar]
  14. Williams J. P., Khan M., Leung S. Biosynthesis of digalactosyl diglyceride in Vicia faba leaves. J Lipid Res. 1975 Jan;16(1):61–66. [PubMed] [Google Scholar]
  15. Williams J. P., Watson G. R., Khan M. U., Leung S. Galactolipid Synthesis in Vicia faba Leaves: I. Galactose, Glycerol, and Fatty Acid Labeling after CO(2) Feeding. Plant Physiol. 1975 Jun;55(6):1038–1042. doi: 10.1104/pp.55.6.1038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Williams J. P., Watson G. R., Leung S. P. Galactolipid Synthesis in Vicia faba Leaves: II. Formation and Desaturation of Long Chain Fatty Acids in Phosphatidylcholine, Phosphatidylglycerol, and the Galactolipids. Plant Physiol. 1976 Feb;57(2):179–184. doi: 10.1104/pp.57.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES